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# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

nextflow
.nextflow.log*
.nextflow/

.gitmodules

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# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

[submodule "src/.docker_modules/hicstuff/3.1.3/hicstuff"]
	path = src/.docker_modules/hicstuff/3.1.3/hicstuff
	url = git@github.com:koszullab/hicstuff.git

.reuse/dep5

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Format: https://www.debian.org/doc/packaging-manuals/copyright-format/1.0/
Upstream-Name: nextflow
Upstream-Contact: Laurent Modolo <laurent.modolo@ens-lyon.fr>
Source: https://gitbio.ens-lyon.fr/LBMC/nextflow

# Sample paragraph, commented out:
#
# Files: src/*
# Copyright: $YEAR $NAME <$CONTACT>
# License: ...
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<!--
SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>

SPDX-License-Identifier: CC-BY-SA-4.0
-->

# Changelog
All notable changes to this project will be documented in this file.

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<!--
SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>

SPDX-License-Identifier: CC-BY-SA-4.0
-->

# Contributing

When contributing to this repository, please first discuss the change you wish to make via issues,
@@ -139,7 +145,7 @@ If you want to use information within the `file_id` to name outputs in your `scr
      file_prefix = file_id.values()[0]
    break
    default:
      file_id
      file_prefix = file_id
    break
  }
```

LICENSE

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Creative Commons Attribution-ShareAlike 4.0 International

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<!--
SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>

SPDX-License-Identifier: CC-BY-SA-4.0
-->

# nextflow pipeline

This repository is a template and a library repository to help you build nextflow pipeline.
@@ -9,7 +15,7 @@ To get the last commits from this repository into your fork use the following co

For the first time:
```sh
git remote add upstream git@gitbio.ens-lyon.fr::pipelines/nextflow.git
git remote add upstream git@gitbio.ens-lyon.fr:LBMC/nextflow.git
git pull upstream master
```

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# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

*
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# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

*
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# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

*.pdf
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<!--
SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>

SPDX-License-Identifier: CC-BY-SA-4.0
-->

# Building your own pipeline

The goal of this guide is to walk you through the Nextflow pipeline building process you will learn:
@@ -57,10 +63,10 @@ Here is an example of **process**:
```Groovy
process sample_fasta {
  input:
  file fasta
  path fasta

  output:
  file "sample.fasta", emit: fasta_sample
  path "sample.fasta", emit: fasta_sample

  script:
"""
@@ -69,20 +75,20 @@ head ${fasta} > sample.fasta
}
```

We have the process `sample_fasta` that takes fasta `file` input and as output a fasta `file`. The `process` task itself is defined in the `script:` block and within `"""`.
We have the process `sample_fasta` that takes fasta `path` input and as output a fasta `path`. The `process` task itself is defined in the `script:` block and within `"""`.

```Groovy
input:
file fasta
path fasta
```

When we zoom on the `input:` block, we see that we define a variable `fasta` of type `file`.
When we zoom on the `input:` block, we see that we define a variable `fasta` of type `path`.
This means that the `sample_fasta` `process` is going to get a flux of fasta file(s).
Nextflow is going to write a file named as the content of the variable `fasta` in the root of the folder where `script:` is executed.

```Groovy
output:
file "sample.fasta", emit: fasta_sample
path "sample.fasta", emit: fasta_sample
```

At the end of the script, a file named `sample.fasta` is found in the root the folder where `script:` is executed and will be emitted as `fasta_sample`.
@@ -201,7 +207,7 @@ Every `fasta_sampler` process write a `sample.fasta` file. We need to make the n

```Groovy
output:
file "*_sample.fasta", emit: fasta_sample
path "*_sample.fasta", emit: fasta_sample

  script:
"""
@@ -360,7 +366,7 @@ We can add the `fastq_from_bed` step to our `workflow`

```Groovy
workflow {
  sample_fasta(fasta_file)
  fastp(fastq_files)
  fasta_from_bed(fasta_files, bed_files)
}
```
@@ -437,7 +443,7 @@ login@allo-psmn
Then once connected to `allo-psmn`, you can connect to `e5-2667v4comp1`:

```sh
login@e5-2667v4comp1
login@m6142comp2
```

## Set your environment
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<!--
SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>

SPDX-License-Identifier: CC-BY-SA-4.0
-->

# Getting Started

These instructions will get you a copy of the project up and running on your local machine for development and testing purposes.
@@ -34,7 +40,7 @@ singularity run docker://hello-world
To install nextflow on your computer simply run the following command:

```sh
git clone git@gitbio.ens-lyon.fr/LBMC/nextflow
git clone git@gitbio.ens-lyon.fr:LBMC/nextflow.git
cd nextflow/
src/install_nextflow.sh
```
@@ -42,6 +48,7 @@ src/install_nextflow.sh
## Running a toy RNASeq quantification pipeline

To run tests we first need to get a training set

```sh
cd data
git clone https://gitbio.ens-lyon.fr/LBMC/Hub/tiny_dataset.git
@@ -63,14 +70,21 @@ By default le `src/nextflow.config` file define 4 different profiles

- `-profile docker` each process of the pipeline will be executed within a `docker` container locally
- `-profile singularity` each process of the pipeline will be executed within a `singularity` container locally
- `-profile psmn` each process will be sent as a separate job within a `singularity` container on the PSMN
- `-profile psmn` each process will be sent as a separate job within a `charliecloud` container on the PSMN
- `-profile ccin2p3` each process will be sent as a separate job within a `singularity` container on the CCIN2P3

If the containers are not found locally, they are automatically downloaded before running the process. For the PSMN and CCIN2P3, the `singularity` images are downloaded in a shared folder (`/scratch/Bio/singularity` for the PSMN, and `/sps/lbmc/common/singularity/` for the CCIN2P3)

If the containers are not found locally, they are automatically downloaded before running the process. For the PSMN and CCIN2P3, the `singularity` images are downloaded in a shared folder (`/scratch/Bio/charliecloud` for the PSMN, and `/sps/lbmc/common/singularity/` for the CCIN2P3)

### PSMN

To have access to `charliecloud` on the PSMN you need to add the followin path to your `PATH` variable:

```sh
PATH=/Xnfs/abc/charliecloud_bin/:$PATH
```

You can add this line in your `~/.bashrc` or `~/.zshrc` file

When running `nextflow` on the PSMN, we recommend to use `tmux` before launching the pipeline:

```sh
@@ -83,10 +97,9 @@ You can re-attach the `tmux` session, with the command `tmux a` (and press `ctrl

### CCIN2P3

When runnning `nextflow` on the CCIN2P3, you cannot use `tmux`, instead you should send a *daemon* jobs which will launch the `nextflow` command.
When runnning `nextflow` on the CCIN2P3, you cannot use `tmux`, instead you should send a _daemon_ jobs which will launch the `nextflow` command.
You can edit the `src/ccin2p3.pbs` file to personalize your `nextflow` command and send it with the command:

```sh
qsub src/ccin2p3.pbs
```
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SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>

SPDX-License-Identifier: CC-BY-SA-4.0
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SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>

SPDX-License-Identifier: CC-BY-SA-4.0
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SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>

SPDX-License-Identifier: CC-BY-SA-4.0

doc/mail.txt.license

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SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>

SPDX-License-Identifier: CC-BY-SA-4.0
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<!--
SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>

SPDX-License-Identifier: CC-BY-SA-4.0
-->

## Projects using nextflow

This page list existing Nextflow based pipeline projects into different categories
@@ -16,6 +22,7 @@ To add your project to this list, please fork this project, modify this file and
- [https://github.com/LBMC/readthroughpombe](https://github.com/LBMC/readthroughpombe)
- [https://_https://gitlab.biologie.ens-lyon.fr/vvanoost/nextflow](https://_https://gitlab.biologie.ens-lyon.fr/vvanoost/nextflow)
- [https://gitlab.biologie.ens-lyon.fr/elabaron/HIV_project](https://gitlab.biologie.ens-lyon.fr/elabaron/HIV_project)  
- [https://gitbio.ens-lyon.fr/LBMC/Palladino/RNAseq_nextflow](https://gitbio.ens-lyon.fr/LBMC/Palladino/RNAseq_nextflow)

### scRNASeq

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# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

*
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# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

FROM ubuntu:22.04

ENV CITESEQ_VERSION=1.4.5
ENV PACKAGES procps locales gcc libstdc++-10-dev libcurl4 libc6 python3 python3-pip python3-dev

RUN apt-get update && \
    apt-get install -y --no-install-recommends ${PACKAGES} && \
    apt-get clean

RUN locale-gen en_US.UTF-8
ENV LC_ALL=en_US.utf-8
ENV LANG=en_US.utf-8
ENV LC_ALL=C.UTF-8
ENV LANG=C.UTF-8


RUN pip3 install CITE-seq-Count==${CITESEQ_VERSION}
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#!/bin/sh

# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

docker pull lbmc/cite_seq_count:1.4.5
docker build src/.docker_modules/CITE-seq-Count/1.4.5 -t 'lbmc/cite_seq_count:1.4.5'
docker push lbmc/cite_seq_count:1.4.5
# docker buildx build --platform linux/amd64,linux/arm64 -t "lbmc/cite_seq_count:1.4.5" --push src/.docker_modules/CITE-seq-Count/1.4.5
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# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

FROM ubuntu:22.04

ENV PACKAGES procps locales gcc libstdc++-10-dev libcurl4 libc6 python3 python3-pip python3-dev git

RUN apt-get update && \
    apt-get install -y --no-install-recommends ${PACKAGES} && \
    apt-get clean

RUN locale-gen en_US.UTF-8
ENV LC_ALL=en_US.utf-8
ENV LANG=en_US.utf-8
ENV LC_ALL=C.UTF-8
ENV LANG=C.UTF-8

RUN git clone https://github.com/Hoohm/CITE-seq-Count.git \
    && cd CITE-seq-Count \
    && git checkout 1.5.0-alpha \
    && pip3 install requests pooch \
    && pip3 install ./
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#!/bin/sh

# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

docker pull lbmc/cite_seq_count:1.5.0
docker build src/.docker_modules/CITE-seq-Count/1.5.0 -t 'lbmc/cite_seq_count:1.5.0'
docker push lbmc/cite_seq_count:1.5.0
# docker buildx build --platform linux/amd64,linux/arm64 -t "lbmc/cite_seq_count:1.5.0" --push src/.docker_modules/CITE-seq-Count/1.5.0
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# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

FROM quay.io/biocontainers/agat:0.8.0--pl5262hdfd78af_0 
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#!/bin/sh

# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

docker pull lbmc/agat:0.8.0
# docker build src/.docker_modules/agat/0.8.0 -t 'lbmc/agat:0.8.0'
# docker push lbmc/agat:0.8.0
docker buildx build --platform linux/amd64,linux/arm64 -t "lbmc/agat:0.8.0" --push src/.docker_modules/agat/0.8.0
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# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

FROM python:3.9-buster as build
MAINTAINER Laurent Modolo

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#!/bin/sh

# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

docker pull lbmc/alntools:dd96682
docker build src/.docker_modules/alntools/dd96682 -t 'lbmc/alntools:dd96682'
docker push lbmc/alntools:dd96682
# docker build src/.docker_modules/alntools/dd96682 -t 'lbmc/alntools:dd96682'
# docker push lbmc/alntools:dd96682
docker buildx build --platform linux/amd64,linux/arm64 -t "lbmc/alntools:dd96682" --push src/.docker_modules/alntools/dd96682
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# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

FROM ubuntu:18.04
MAINTAINER Laurent Modolo

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#!/bin/sh

# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

docker pull lbmc/bamutils:1.0.14
docker build src/.docker_modules/bamutils/1.0.14 -t 'lbmc/bamutils:1.0.14'
docker push lbmc/bamutils:1.0.14
# docker build src/.docker_modules/bamutils/1.0.14 -t 'lbmc/bamutils:1.0.14'
# docker push lbmc/bamutils:1.0.14
docker buildx build --platform linux/amd64,linux/arm64 -t "lbmc/bamutils:1.0.14" --push src/.docker_modules/bamutils/1.0.14
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# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

FROM ubuntu:18.04
MAINTAINER Laurent Modolo

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#!/bin/sh

# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

docker pull lbmc/bcftools:1.7
docker build src/.docker_modules/bcftools/1.7 -t 'lbmc/bcftools:1.7'
docker push lbmc/bcftools:1.7
# docker build src/.docker_modules/bcftools/1.7 -t 'lbmc/bcftools:1.7'
# docker push lbmc/bcftools:1.7
docker buildx build --platform linux/amd64,linux/arm64 -t "lbmc/bcftools:1.7" --push src/.docker_modules/bcftools/1.7
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# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

FROM quay.io/biocontainers/bedops:2.4.39--hc9558a2_0
MAINTAINER Laurent Modolo
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#!/bin/sh

# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

docker pull lbmc/bedops:2.4.39
docker build src/.docker_modules/bedops/2.4.39 -t 'lbmc/bedops:2.4.39'
docker push lbmc/bedops:2.4.39
# docker build src/.docker_modules/bedops/2.4.39 -t 'lbmc/bedops:2.4.39'
# docker push lbmc/bedops:2.4.39
docker buildx build --platform linux/amd64,linux/arm64 -t "lbmc/bedops:2.4.39" --push src/.docker_modules/bedops/2.4.39
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# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

FROM ubuntu:16.04
MAINTAINER Laurent Modolo

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#!/bin/sh

# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

docker pull lbmc/bedtools:2.25.0
docker build src/.docker_modules/bedtools/2.25.0 -t 'lbmc/bedtools:2.25.0'
docker push lbmc/bedtools:2.25.0
# docker build src/.docker_modules/bedtools/2.25.0 -t 'lbmc/bedtools:2.25.0'
# docker push lbmc/bedtools:2.25.0
docker buildx build --platform linux/amd64,linux/arm64 -t "lbmc/bedtools:2.25.0" --push src/.docker_modules/bedtools/2.25.0
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# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

FROM quay.io/biocontainers/bedtools:2.30.0--h7d7f7ad_1
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#!/bin/sh

# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

docker pull lbmc/bedtools:2.30.0
docker build src/.docker_modules/bedtools/2.30.0 -t 'lbmc/bedtools:2.30.0'
docker push lbmc/bedtools:2.30.0
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# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

FROM ubuntu:18.04
MAINTAINER Laurent Modolo

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#!/bin/sh

# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

docker pull lbmc/bioawk:1.0
docker build src/.docker_modules/bioawk/1.0 -t 'lbmc/bioawk:1.0'
docker push lbmc/bioawk:1.0
# docker build src/.docker_modules/bioawk/1.0 -t 'lbmc/bioawk:1.0'
# docker push lbmc/bioawk:1.0
docker buildx build --platform linux/amd64,linux/arm64 -t "lbmc/bioawk:1.0" --push src/.docker_modules/bioawk/1.0
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# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

FROM bioconvert/bioconvert:test
MAINTAINER Laurent Modolo

ENV BIOCONVERT_VERSION="0.4.0"
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#!/bin/sh

# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

docker pull lbmc/bioconvert:0.4.0
# docker build src/.docker_modules/bioconvert/0.4.0 -t 'lbmc/bioconvert:0.4.0'
# docker push lbmc/bioconvert:0.4.0
docker buildx build --platform linux/amd64,linux/arm64 -t "lbmc/bioconvert:0.4.0" --push src/.docker_modules/bioconvert/0.4.0
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# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

FROM conda/miniconda3
MAINTAINER Laurent Modolo

ENV BIOCONVERT_VERSION="0.4.3"
RUN conda init \
&& conda config --add channels r \
&& conda config --add channels defaults \
&& conda config --add channels conda-forge \
&& conda config --add channels bioconda \
&& conda create -y -n bioconvert
SHELL ["conda", "run", "-n", "bioconvert", "/bin/bash", "-c"]
RUN conda install bioconvert \
&& echo "conda activate bioconvert" >> /root/.bashrc
RUN apt update && apt install -y procps

ENV PATH /usr/local/envs/bioconvert/bin:/usr/local/condabin:$PATH
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#!/bin/sh

# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

docker pull lbmc/bioconvert:0.4.3
# docker build src/.docker_modules/bioconvert/0.4.3 -t 'lbmc/bioconvert:0.4.3'
# docker push lbmc/bioconvert:0.4.3
docker buildx build --platform linux/amd64,linux/arm64 -t "lbmc/bioconvert:0.4.3" --push src/.docker_modules/bioconvert/0.4.3
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# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

FROM ubuntu:18.04
MAINTAINER Laurent Modolo

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#!/bin/sh

# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

docker pull lbmc/bowtie:1.2.2
docker build src/.docker_modules/bowtie/1.2.2 -t 'lbmc/bowtie:1.2.2'
docker push lbmc/bowtie:1.2.2
# docker build src/.docker_modules/bowtie/1.2.2 -t 'lbmc/bowtie:1.2.2'
# docker push lbmc/bowtie:1.2.2
docker buildx build --platform linux/amd64,linux/arm64 -t "lbmc/bowtie:1.2.2" --push src/.docker_modules/bowtie/1.2.2
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# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

FROM ubuntu:18.04
MAINTAINER Laurent Modolo

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#!/bin/sh

# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

docker pull lbmc/bowtie2:2.3.4.1
docker build src/.docker_modules/bowtie2/2.3.4.1 -t 'lbmc/bowtie2:2.3.4.1'
docker push lbmc/bowtie2:2.3.4.1
# docker build src/.docker_modules/bowtie2/2.3.4.1 -t 'lbmc/bowtie2:2.3.4.1'
# docker push lbmc/bowtie2:2.3.4.1
docker buildx build --platform linux/amd64,linux/arm64 -t "lbmc/bowtie2:2.3.4.1" --push src/.docker_modules/bowtie2/2.3.4.1
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# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

FROM lbmc/samtools:1.11
MAINTAINER Laurent Modolo

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#!/bin/sh

# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

docker pull lbmc/bwa:0.7.17
docker build src/.docker_modules/bwa/0.7.17 -t 'lbmc/bwa:0.7.17'
docker push lbmc/bwa:0.7.17
# docker build src/.docker_modules/bwa/0.7.17 -t 'lbmc/bwa:0.7.17'
# docker push lbmc/bwa:0.7.17
docker buildx build --platform linux/amd64,linux/arm64 -t "lbmc/bwa:0.7.17" --push src/.docker_modules/bwa/0.7.17
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# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

FROM ubuntu:18.04
MAINTAINER Laurent Modolo

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#!/bin/sh

# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

docker pull lbmc/canu:1.6
docker build src/.docker_modules/canu/1.6 -t 'lbmc/canu:1.6'
docker push lbmc/canu:1.6
# docker build src/.docker_modules/canu/1.6 -t 'lbmc/canu:1.6'
# docker push lbmc/canu:1.6
docker buildx build --platform linux/amd64,linux/arm64 -t "lbmc/canu:1.6" --push src/.docker_modules/canu/1.6
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# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

FROM python:3.7-slim 

ENV RPY2_CFFI_MODE=ABI

RUN mkdir /app /docker
COPY requirements.txt /app
COPY requirements-web.txt /app
WORKDIR /app

RUN pip install -r requirements.txt
RUN pip install -r requirements-web.txt
RUN pip install cellphonedb==3.0.0


COPY . /app
COPY ./docker /docker
RUN mkdir -p /var/log/uwsgi
RUN touch /var/log/uwsgi/cellphonedb.log
RUN chmod +x /app

CMD ["/docker/run-system.sh"]
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[uwsgi]
module = wsgi:app

master = true
processes = 4


socket = 0.0.0.0:5000
chmod-socket = 660
vacuum = true

die-on-term = true

stats = /tmp/stats.socket
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# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

FROM postgres:9.6

ENV POSTGRES_PASSWORD  root
ENV POSTGRES_USER root
ENV POSTGRES_DB cellphonedb
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#!/bin/bash

# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

exec uwsgi --ini cellphonedb.ini --log-master
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click>=7.0,<8.0
pandas>=1.0.0,<=1.1.4
numpy>=1.16.5,<=1.19.5
numpy-groupies
scikit-learn==0.22
flask>=1.0,<2.0.0
Flask-RESTful>=0.3
Flask-Testing>=0.7
SQLAlchemy>=1.3,<1.4
PyYAML>=5.4,<6.0
requests>=2.25,<3.0
pika>=1.0.0,<2.0
boto3>=1.15,<2.0
geosketch==0.3
rpy2>=3.4.0,<4.0
tqdm>=4.3,<5.0
h5py<3.0.0
anndata>=0.7,<=0.75
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# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

FROM debian:stretch-slim

ENV CLUSTALO_VERSION=1.2.4-1
ENV PACKAGES clustalo=${CLUSTALO_VERSION} \
  procps 

RUN apt-get update && \
    apt-get install -y --no-install-recommends ${PACKAGES} && \
    apt-get clean
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#!/bin/sh

# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

docker pull lbmc/clustalo:1.2.4
# docker build src/.docker_modules/clustalo/1.2.4 -t 'lbmc/clustalo:1.2.4'
# docker push lbmc/clustalo:1.2.4
docker buildx build --platform linux/amd64,linux/arm64 -t "lbmc/clustalo:1.2.4" --push src/.docker_modules/clustalo/1.2.4
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# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

FROM quay.io/biocontainers/crossmap:0.4.1--pyh5ca1d4c_0
MAINTAINER Laurent Modolo
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#!/bin/sh

# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

docker pull lbmc/crossmap:0.4.1
docker build src/.docker_modules/crossmap/0.4.1/ -t 'lbmc/crossmap:0.4.1'
docker push lbmc/crossmap:0.4.1
# docker build src/.docker_modules/crossmap/0.4.1/ -t 'lbmc/crossmap:0.4.1'
# docker push lbmc/crossmap:0.4.1
docker buildx build --platform linux/amd64,linux/arm64 -t "lbmc/crossmap:0.4.1" --push src/.docker_modules/crossmap/0.4.1
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# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

FROM alpine:3.8
MAINTAINER Laurent Modolo

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#!/bin/sh

# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

docker pull lbmc/cutadapt:1.14
docker build src/.docker_modules/cutadapt/1.14 -t 'lbmc/cutadapt:1.14'
docker push lbmc/cutadapt:1.14
# docker build src/.docker_modules/cutadapt/1.14 -t 'lbmc/cutadapt:1.14'
# docker push lbmc/cutadapt:1.14
docker buildx build --platform linux/amd64,linux/arm64 -t "lbmc/cutadapt:1.14" --push src/.docker_modules/cutadapt/1.14
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# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

FROM alpine:3.8
MAINTAINER Laurent Modolo

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#!/bin/sh

# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

docker pull lbmc/cutadapt:1.15
docker build src/.docker_modules/cutadapt/1.15 -t 'lbmc/cutadapt:1.15'
docker push lbmc/cutadapt:1.15
# docker build src/.docker_modules/cutadapt/1.15 -t 'lbmc/cutadapt:1.15'
# docker push lbmc/cutadapt:1.15
docker buildx build --platform linux/amd64,linux/arm64 -t "lbmc/cutadapt:1.15" --push src/.docker_modules/cutadapt/1.15
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# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

FROM alpine:3.8
MAINTAINER Laurent Modolo

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#!/bin/sh

# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

docker pull lbmc/cutadapt:2.1
docker build src/.docker_modules/cutadapt/2.1 -t 'lbmc/cutadapt:2.1'
docker push lbmc/cutadapt:2.1
# docker build src/.docker_modules/cutadapt/2.1 -t 'lbmc/cutadapt:2.1'
# docker push lbmc/cutadapt:2.1
docker buildx build --platform linux/amd64,linux/arm64 -t "lbmc/cutadapt:2.1" --push src/.docker_modules/cutadapt/2.1
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# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

FROM ubuntu:20.04 

ENV DANPOS_VERSION="2f7f223"
ENV TZ=Europe/Paris
RUN ln -snf /usr/share/zoneinfo/$TZ /etc/localtime && echo $TZ > /etc/timezone \
&& apt update -qq \
&& apt install --no-install-recommends software-properties-common -y dirmngr git python \
&& wget -qO- https://cloud.r-project.org/bin/linux/ubuntu/marutter_pubkey.asc | tee -a /etc/apt/trusted.gpg.d/cran_ubuntu_key.asc \
&& add-apt-repository "deb https://cloud.r-project.org/bin/linux/ubuntu $(lsb_release -cs)-cran40/"
RUN apt-get update \
&& apt-get install -y r-base=4.0.1 samtools \
&& git clone https://github.com/sklasfeld/DANPOS3.git \
&& cd DANPOS3 \
&& git checkout $DANPOS_VERSION \
&& pip install -r requirements.txt \
&& chmod +x DANPOS3/*.py

ENV PATH="/DANPOS3:${PATH}"
CMD [ "bash" ]
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#!/bin/sh

# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

docker pull lbmc/danpos3:2f7f223
# docker build src/.docker_modules/danpos3/2f7f223 -t 'lbmc/danpos3:2f7f223'
# docker push lbmc/danpos3:2f7f223
docker buildx build --platform linux/amd64,linux/arm64 -t "lbmc/danpos3:2f7f223" --push src/.docker_modules/danpos3/2f7f223
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# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

FROM debian:stretch
MAINTAINER Laurent Modolo

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#!/bin/sh

# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

docker pull lbmc/deeptools:3.0.2
docker build src/.docker_modules/deeptools/3.0.2 -t 'lbmc/deeptools:3.0.2'
docker push lbmc/deeptools:3.0.2
# docker build src/.docker_modules/deeptools/3.0.2 -t 'lbmc/deeptools:3.0.2'
# docker push lbmc/deeptools:3.0.2
docker buildx build --platform linux/amd64,linux/arm64 -t "lbmc/deeptools:3.0.2" --push src/.docker_modules/deeptools/3.0.2
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# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

FROM debian:stretch
MAINTAINER Laurent Modolo

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#!/bin/sh

# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

docker pull lbmc/deeptools:3.1.1
docker build src/.docker_modules/deeptools/3.1.1 -t 'lbmc/deeptools:3.1.1'
docker push lbmc/deeptools:3.1.1
# docker build src/.docker_modules/deeptools/3.1.1 -t 'lbmc/deeptools:3.1.1'
# docker push lbmc/deeptools:3.1.1
docker buildx build --platform linux/amd64,linux/arm64 -t "lbmc/deeptools:3.1.1" --push src/.docker_modules/deeptools/3.1.1
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# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

FROM python:3.9-slim
MAINTAINER Lauret Modolo

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#!/bin/sh

# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

docker pull lbmc/deeptools:3.5.0
docker build src/.docker_modules/deeptools/3.5.0 -t 'lbmc/deeptools:3.5.0'
docker push lbmc/deeptools:3.5.0
# docker build src/.docker_modules/deeptools/3.5.0 -t 'lbmc/deeptools:3.5.0'
# docker push lbmc/deeptools:3.5.0
docker buildx build --platform linux/amd64,linux/arm64 -t "lbmc/deeptools:3.5.0" --push src/.docker_modules/deeptools/3.5.0
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# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

FROM python:3.8-slim
MAINTAINER Lauret Modolo

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#!/bin/sh

# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

docker pull lbmc/deeptools:3.5.1
docker build src/.docker_modules/deeptools/3.5.1 -t 'lbmc/deeptools:3.5.1'
docker push lbmc/deeptools:3.5.1
# docker build src/.docker_modules/deeptools/3.5.1 -t 'lbmc/deeptools:3.5.1'
# docker push lbmc/deeptools:3.5.1
docker buildx build --platform linux/amd64,linux/arm64 -t "lbmc/deeptools:3.5.1" --push src/.docker_modules/deeptools/3.5.1
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#!/bin/sh

# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

fd "Dockerfile" src/.docker_modules | perl -pe 's|.*docker_modules/(.*)/(.*)/Dockerfile|\1:\2|g' | awk '{system("docker push lbmc/"$0)}'
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#!/bin/sh

# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

fd "Dockerfile" src/.docke_modules | perl -pe 's|.*docker_modules/(.*)/(.*)/Dockerfile|\1:\2|g' | awk '{system("docker tag "$0" lbmc/" $0)}'
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# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

FROM debian:buster as build
MAINTAINER Laurent Modolo

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#!/bin/sh

# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

docker pull lbmc/emase-zero:0.3.1
docker build src/.docker_modules/emase-zero/0.3.1 -t 'lbmc/emase-zero:0.3.1'
docker push lbmc/emase-zero:0.3.1
# docker build src/.docker_modules/emase-zero/0.3.1 -t 'lbmc/emase-zero:0.3.1'
# docker push lbmc/emase-zero:0.3.1
docker buildx build --platform linux/amd64,linux/arm64 -t "lbmc/emase-zero:0.3.1" --push src/.docker_modules/emase-zero/0.3.1
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FROM python:2.7
# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

FROM conda/miniconda2
MAINTAINER Laurent Modolo

ENV EMASE_VERSION=0.10.16
RUN conda init \
&& conda config --add channels r \
&& conda config --add channels bioconda \
&& conda create -y -n emase jupyter
SHELL ["conda", "run", "-n", "emase", "/bin/bash", "-c"]
RUN conda install -y -c kbchoi emase \
&& echo "conda activate emase" >> /root/.bashrc
RUN apt update && apt install -y procps

RUN apt-get update \
    && apt-get install -y python-h5py \
    && pip install numpy==1.8.2 \
    && pip install numexpr==2.3.1 \
    && pip install cython>=0.13 \
    && pip install tables==3.1.0 \
    && pip install emase==${EMASE_VERSION}

CMD ["bash"]
ENV PATH /usr/local/envs/emase/bin:/usr/local/condabin:$PATH
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#!/bin/sh

# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

docker pull lbmc/emase:0.10.16
docker build src/.docker_modules/emase/0.10.16 -t 'lbmc/emase:0.10.16'
docker push lbmc/emase:0.10.16
# docker build src/.docker_modules/emase/0.10.16 -t 'lbmc/emase:0.10.16'
# docker push lbmc/emase:0.10.16
docker buildx build --platform linux/amd64,linux/arm64 -t "lbmc/emase:0.10.16" --push src/.docker_modules/emase/0.10.16
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# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

FROM quay.io/biocontainers/fastp:0.19.7--hdbcaa40_0
MAINTAINER Laurent Modolo
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#!/bin/sh

# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

docker pull lbmc/fastp:0.19.7
docker build src/.docker_modules/fastp/0.19.7 -t 'lbmc/fastp:0.19.7'
docker push lbmc/fastp:0.19.7
# docker build src/.docker_modules/fastp/0.19.7 -t 'lbmc/fastp:0.19.7'
# docker push lbmc/fastp:0.19.7
docker buildx build --platform linux/amd64,linux/arm64 -t "lbmc/fastp:0.19.7" --push src/.docker_modules/fastp/0.19.7
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# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

FROM quay.io/biocontainers/fastp:0.20.1--h8b12597_0
MAINTAINER Laurent Modolo
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#!/bin/sh

# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

docker pull lbmc/fastp:0.20.1
docker build src/.docker_modules/fastp/0.20.1 -t 'lbmc/fastp:0.20.1'
docker push lbmc/fastp:0.20.1
# docker build src/.docker_modules/fastp/0.20.1 -t 'lbmc/fastp:0.20.1'
# docker push lbmc/fastp:0.20.1
docker buildx build --platform linux/amd64,linux/arm64 -t "lbmc/fastp:0.20.1" --push src/.docker_modules/fastp/0.20.1
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# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

FROM quay.io/biocontainers/fastp:0.23.2--hb7a2d85_2
MAINTAINER Laurent Modolo
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#!/bin/sh

# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

docker pull lbmc/fastp:0.23.2
# docker build src/.docker_modules/fastp/0.23.2 -t 'lbmc/fastp:0.23.2'
docker buildx build --platform linux/amd64,linux/arm64 -t "lbmc/fastp:0.23.2" --push src/.docker_modules/fastp/0.23.2
docker push lbmc/fastp:0.23.2
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# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

FROM ubuntu:18.04
MAINTAINER Laurent Modolo

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#!/bin/sh

# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

docker pull lbmc/fastqc:0.11.5
docker build src/.docker_modules/fastqc/0.11.5 -t 'lbmc/fastqc:0.11.5'
docker push lbmc/fastqc:0.11.5
# docker build src/.docker_modules/fastqc/0.11.5 -t 'lbmc/fastqc:0.11.5'
# docker push lbmc/fastqc:0.11.5
docker buildx build --platform linux/amd64,linux/arm64 -t "lbmc/fastqc:0.11.5" --push src/.docker_modules/fastqc/0.11.5
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# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

FROM ubuntu:18.04
MAINTAINER Laurent Modolo

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#!/bin/sh

# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

docker pull lbmc/file_handle:0.1.1
docker build src/.docker_modules/file_handle/0.1.1 -t 'lbmc/file_handle:0.1.1'
docker push lbmc/file_handle:0.1.1
# docker build src/.docker_modules/file_handle/0.1.1 -t 'lbmc/file_handle:0.1.1'
# docker push lbmc/file_handle:0.1.1
docker buildx build --platform linux/amd64,linux/arm64 -t "lbmc/file_handle:0.1.1" --push src/.docker_modules/file_handle/0.1.1
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# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later

FROM python:3.9-slim
MAINTAINER Lauret Modolo

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