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Commit e27a0df3 authored by Arnaud Duvermy's avatar Arnaud Duvermy
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Update README.md

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......@@ -10,7 +10,7 @@ Purpose:
Main step:
1) Estimate size factor
### 1) Estimate size factor
Median ratio method is used to estimate the size factor per sample.
......@@ -19,14 +19,14 @@ Normalized counts allow minimizing biais linked to library size.
By normalizing the counts DESEQ2 aims to make sure differential expression are based on factors study and not to sequencing depth
/!\ gene length is not take into account !
2) Estimate dispersion
### 2)Estimate dispersion
Purpose: Estimate the variability between replicates
Purpose: Estimate the variability between replicates <br/>
Get dispersion estimate for each gene using Maximum Linkelihood Estimatation
Get dispersion estimate for each gene using Maximum Linkelihood Estimatation <br/>
Fit a curve to wise gene dispersion estimate
3) Fit linear model
### 3) Fit linear model
The differential expression analysis uses a generalized linear model of the form:
Kij ∼ NB(µij , α i )<br/>
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