From e27a0df32516c12527a53f18b5bb903c39d7b4c0 Mon Sep 17 00:00:00 2001 From: Arnaud Duvermy <arnaud.duvermy@ens-lyon.fr> Date: Thu, 10 Feb 2022 13:44:13 +0100 Subject: [PATCH] Update README.md --- README.md | 10 +++++----- 1 file changed, 5 insertions(+), 5 deletions(-) diff --git a/README.md b/README.md index ecf461c..aeb7303 100644 --- a/README.md +++ b/README.md @@ -10,7 +10,7 @@ Purpose: Main step: -1) Estimate size factor +### 1) Estimate size factor Median ratio method is used to estimate the size factor per sample. @@ -19,14 +19,14 @@ Normalized counts allow minimizing biais linked to library size. By normalizing the counts DESEQ2 aims to make sure differential expression are based on factors study and not to sequencing depth /!\ gene length is not take into account ! -2) Estimate dispersion +### 2)Estimate dispersion -Purpose: Estimate the variability between replicates +Purpose: Estimate the variability between replicates <br/> -Get dispersion estimate for each gene using Maximum Linkelihood Estimatation +Get dispersion estimate for each gene using Maximum Linkelihood Estimatation <br/> Fit a curve to wise gene dispersion estimate -3) Fit linear model +### 3) Fit linear model The differential expression analysis uses a generalized linear model of the form: Kij ∼ NB(µij , α i )<br/> -- GitLab