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Commit df5a0243 authored by nservant's avatar nservant
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[DOC] update README

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...@@ -24,9 +24,9 @@ In practice, this workflow was successfully applied to many data-sets including ...@@ -24,9 +24,9 @@ In practice, this workflow was successfully applied to many data-sets including
dilution Hi-C, in situ Hi-C, DNase Hi-C, Micro-C, capture-C, capture Hi-C or dilution Hi-C, in situ Hi-C, DNase Hi-C, Micro-C, capture-C, capture Hi-C or
HiChip data. HiChip data.
Contact maps are generated in standard formats including HiC-Pro, cooler, and h5 format for Contact maps are generated in standard formats including HiC-Pro, and cooler for
downstream analysis and visualization. downstream analysis and visualization.
Addition analysis steps such as TADs calling are also available. Addition analysis steps such as compartments and TADs calling are also available.
The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool
to run tasks across multiple compute infrastructures in a very portable manner. to run tasks across multiple compute infrastructures in a very portable manner.
...@@ -35,15 +35,18 @@ results highly reproducible. ...@@ -35,15 +35,18 @@ results highly reproducible.
## Pipeline summary ## Pipeline summary
1. Mapping using a two steps strategy to rescue reads spanning the ligation 1. HiC-Pro data processing [`HiC-Pro`](https://github.com/nservant/HiC-Pro)
sites ([`bowtie2`](http://bowtie-bio.sourceforge.net/bowtie2/index.shtml)) 1. Mapping using a two steps strategy to rescue reads spanning the ligation
2. Detection of valid interaction products([`HiC-Pro`](https://github.com/nservant/HiC-Pro)) sites ([`bowtie2`](http://bowtie-bio.sourceforge.net/bowtie2/index.shtml))
3. Duplicates removal 2. Detection of valid interaction products
4. Create genome-wide contact maps at various resolution ([`cooler`](https://github.com/open2c/cooler)) 3. Duplicates removal
5. Contact maps normalization using the ICE algorithm ([`cooler`](https://github.com/open2c/cooler)) 4. Generate raw and normalized contact maps ([`iced`](https://github.com/hiclib/iced))
6. Export to various contact maps formats ([`HiC-Pro`](https://github.com/nservant/HiC-Pro), [`cooler`](https://github.com/open2c/cooler), [`HiCExplorer`](https://github.com/deeptools/HiCExplorer)) 2. Create genome-wide contact maps at various resolution ([`cooler`](https://github.com/open2c/cooler))
7. Quality controls ([`HiC-Pro`](https://github.com/nservant/HiC-Pro), [`HiCExplorer`](https://github.com/deeptools/HiCExplorer)) 3. Contact maps normalization using balancing algorithm ([`cooler`](https://github.com/open2c/cooler))
8. TADs calling ([`HiCExplorer`](https://github.com/deeptools/HiCExplorer), [`cooler`](https://github.com/open2c/cooler)) 4. Export to various contact maps formats ([`HiC-Pro`](https://github.com/nservant/HiC-Pro), [`cooler`](https://github.com/open2c/cooler))
5. Quality controls ([`HiC-Pro`](https://github.com/nservant/HiC-Pro), [`HiCExplorer`](https://github.com/deeptools/HiCExplorer))
6. Compartments calling ([`cooltools`](https://cooltools.readthedocs.io/en/latest/))
8. TADs calling ([`HiCExplorer`](https://github.com/deeptools/HiCExplorer), [`cooltools`](https://cooltools.readthedocs.io/en/latest/))
9. Quality control report ([`MultiQC`](https://multiqc.info/)) 9. Quality control report ([`MultiQC`](https://multiqc.info/))
## Quick Start ## Quick Start
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