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Commit df5a0243 authored by nservant's avatar nservant
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[DOC] update README

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......@@ -24,9 +24,9 @@ In practice, this workflow was successfully applied to many data-sets including
dilution Hi-C, in situ Hi-C, DNase Hi-C, Micro-C, capture-C, capture Hi-C or
HiChip data.
Contact maps are generated in standard formats including HiC-Pro, cooler, and h5 format for
Contact maps are generated in standard formats including HiC-Pro, and cooler for
downstream analysis and visualization.
Addition analysis steps such as TADs calling are also available.
Addition analysis steps such as compartments and TADs calling are also available.
The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool
to run tasks across multiple compute infrastructures in a very portable manner.
......@@ -35,15 +35,18 @@ results highly reproducible.
## Pipeline summary
1. Mapping using a two steps strategy to rescue reads spanning the ligation
sites ([`bowtie2`](http://bowtie-bio.sourceforge.net/bowtie2/index.shtml))
2. Detection of valid interaction products([`HiC-Pro`](https://github.com/nservant/HiC-Pro))
3. Duplicates removal
4. Create genome-wide contact maps at various resolution ([`cooler`](https://github.com/open2c/cooler))
5. Contact maps normalization using the ICE algorithm ([`cooler`](https://github.com/open2c/cooler))
6. Export to various contact maps formats ([`HiC-Pro`](https://github.com/nservant/HiC-Pro), [`cooler`](https://github.com/open2c/cooler), [`HiCExplorer`](https://github.com/deeptools/HiCExplorer))
7. Quality controls ([`HiC-Pro`](https://github.com/nservant/HiC-Pro), [`HiCExplorer`](https://github.com/deeptools/HiCExplorer))
8. TADs calling ([`HiCExplorer`](https://github.com/deeptools/HiCExplorer), [`cooler`](https://github.com/open2c/cooler))
1. HiC-Pro data processing [`HiC-Pro`](https://github.com/nservant/HiC-Pro)
1. Mapping using a two steps strategy to rescue reads spanning the ligation
sites ([`bowtie2`](http://bowtie-bio.sourceforge.net/bowtie2/index.shtml))
2. Detection of valid interaction products
3. Duplicates removal
4. Generate raw and normalized contact maps ([`iced`](https://github.com/hiclib/iced))
2. Create genome-wide contact maps at various resolution ([`cooler`](https://github.com/open2c/cooler))
3. Contact maps normalization using balancing algorithm ([`cooler`](https://github.com/open2c/cooler))
4. Export to various contact maps formats ([`HiC-Pro`](https://github.com/nservant/HiC-Pro), [`cooler`](https://github.com/open2c/cooler))
5. Quality controls ([`HiC-Pro`](https://github.com/nservant/HiC-Pro), [`HiCExplorer`](https://github.com/deeptools/HiCExplorer))
6. Compartments calling ([`cooltools`](https://cooltools.readthedocs.io/en/latest/))
8. TADs calling ([`HiCExplorer`](https://github.com/deeptools/HiCExplorer), [`cooltools`](https://cooltools.readthedocs.io/en/latest/))
9. Quality control report ([`MultiQC`](https://multiqc.info/))
## Quick Start
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