src/RNAseq.nf : no softclip and trim 1base at 5' and 3' end by hisat

src/RNAseq.nf

@@ -289,6 +289,9 @@ hisat2 -x ${index_id} \
        --un-conc-gz ${file_id}_notaligned_R%.fastq.gz \
        --rna-strandness ${params.strand} \
        --dta \
+       --no-softclip\
+       --trim3 1\
+       --trim5 1\
        2> ${file_id}_genome.txt \
 | samtools view -bS -F 4 - \
 | samtools sort -@ ${task.cpus} -o ${file_id}.bam \