diff --git a/src/RNAseq.nf b/src/RNAseq.nf index 97a95fe2d3caca8c7afe5e0327b14952a7d217ed..e1d9f8729e10a68a67850ac2ddde4992df2cbf7f 100644 --- a/src/RNAseq.nf +++ b/src/RNAseq.nf @@ -289,6 +289,9 @@ hisat2 -x ${index_id} \ --un-conc-gz ${file_id}_notaligned_R%.fastq.gz \ --rna-strandness ${params.strand} \ --dta \ + --no-softclip\ + --trim3 1\ + --trim5 1\ 2> ${file_id}_genome.txt \ | samtools view -bS -F 4 - \ | samtools sort -@ ${task.cpus} -o ${file_id}.bam \