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Xavier Grand
ChIPster
Commits
c98150b9
Verified
Commit
c98150b9
authored
3 years ago
by
Laurent Modolo
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fastp: add accel_1splus protocol trimming with a switch
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4a7355a2
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src/nf_modules/fastp/main.nf
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c98150b9
version = "0.20.1"
container_url = "lbmc/fastp:${version}"
params.fastp_protocol = ""
params.fastp = ""
params.fastp_pairedend = ""
params.fastp_singleend = ""
process fastp {
workflow fastp {
take:
fastq
main:
switch(params.fastp_protocol) {
case "accel_1splus":
fastp_accel_1splus(fastq)
fastp_accel_1splus.out.fastq.set{res_fastq}
fastp_accel_1splus.out.report.set{res_report}
break;
default:
fastp_default(fastq)
fastp_default.out.fastq.set{res_fastq}
fastp_default.out.report.set{res_report}
break;
}
emit:
fastq = res_fastq
report = res_report
}
process fastp_default {
container = "${container_url}"
label "big_mem_multi_cpus"
tag "$pair_id"
...
...
@@ -50,6 +73,68 @@ ${params.fastp} \
"""
}
process fastp_accel_1splus {
container = "${container_url}"
label "big_mem_multi_cpus"
tag "$pair_id"
publishDir "results/QC/fastp/", mode: 'copy', pattern: "*.html"
input:
tuple val(pair_id), path(reads)
output:
tuple val(pair_id), path("*.fastq.gz"), emit: fastq
tuple val(pair_id), path("*.html"), emit: html
tuple val(pair_id), path("*.json"), emit: report
script:
if (reads instanceof List)
"""
fastp --thread ${task.cpus} \
--disable_quality_filtering \
--disable_length_filtering \
--disable_trim_poly_g \
--stdout \
--in1 ${reads[0]} \
--in2 ${reads[1]} \
--out1 ${pair_id}_R1_trim.fastq.gz \
--out2 ${pair_id}_R2_trim.fastq.gz | \
fastp --thread ${task.cpus} \
--stdin \
--trim_front1=10 \
--trim_tail1=10 \
--trim_tail2=10 \
--qualified_quality_phred 20 \
--disable_length_filtering \
--detect_adapter_for_pe \
${params.fastp} \
--html ${pair_id}.html \
--json ${pair_id}_fastp.json \
--report_title ${pair_id}
"""
else
"""
fastp --thread ${task.cpus} \
--disable_quality_filtering \
--disable_length_filtering \
--disable_trim_poly_g \
--stdout \
--in1 ${reads[0]} \
--out1 ${pair_id}_R1_trim.fastq.gz \
fastp --thread ${task.cpus} \
--stdin \
--trim_front1=10 \
--trim_tail1=10 \
--qualified_quality_phred 20 \
--disable_length_filtering \
--detect_adapter_for_pe \
${params.fastp} \
--html ${pair_id}.html \
--json ${pair_id}_fastp.json \
--report_title ${pair_id}
"""
}
process fastp_pairedend {
container = "${container_url}"
label "big_mem_multi_cpus"
...
...
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