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vvanoost
nextflow
Commits
9f0502eb
Commit
9f0502eb
authored
6 years ago
by
vvanoost
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parent
30e9022a
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1 changed file
src/RNAseq_sen1D_bowtie2_SE_CCA.nf
+10
-10
10 additions, 10 deletions
src/RNAseq_sen1D_bowtie2_SE_CCA.nf
with
10 additions
and
10 deletions
src/RNAseq_sen1D_bowtie2_SE_CCA.nf
+
10
−
10
View file @
9f0502eb
...
...
@@ -22,7 +22,7 @@ fastq_files.into{fastq_files_adaptor; fastq_files_fastq}
process
fastqc_fastq
{
tag
"$reads.baseName"
publishDir
"results/
fastq_SE
/fastqc/raw"
,
mode:
'copy'
publishDir
"results/
SE_CCA_sequencing/fastq_SE_filtered
/fastqc/raw"
,
mode:
'copy'
input:
file
reads
from
fastq_files_fastq
...
...
@@ -57,7 +57,7 @@ fastq_files_cut.into{fastq_files_cut_randombp; fastq_files_cut_fastq}
process
fastqc_fastq_cutadapt
{
tag
"$reads.baseName"
publishDir
"results/
fastq_SE
/fastqc/adaptor_removal/"
,
mode:
'copy'
publishDir
"results/
SE_CCA_sequencing/fastq_SE_filtered
/fastqc/adaptor_removal/"
,
mode:
'copy'
input:
file
(
reads
)
from
fastq_files_cut_fastq
...
...
@@ -73,7 +73,7 @@ fastqc --quiet --threads ${task.cpus} --format fastq --outdir ./ ${reads}
process
random_bases_4_trimming
{
tag
"$reads.baseName"
publishDir
"results/
fastq_SE
/adaptor_removal/"
,
mode:
'copy'
publishDir
"results/
SE_CCA_sequencing/fastq_SE_filtered
/adaptor_removal/"
,
mode:
'copy'
input:
file
reads
from
fastq_files_cut_randombp
...
...
@@ -93,7 +93,7 @@ fastq_files_cut4.into{fastq_files_trim; fastq_files_cut4_fastq}
process
fastqc_fastq_randombp
{
tag
"$reads.baseName"
publishDir
"results/
fastq_SE
/fastqc/random_bases_4_trimming/"
,
mode:
'copy'
publishDir
"results/
SE_CCA_sequencing/fastq_SE_filtered
/fastqc/random_bases_4_trimming/"
,
mode:
'copy'
input:
file
reads
from
fastq_files_cut4_fastq
...
...
@@ -121,7 +121,7 @@ fastqc --quiet --threads ${task.cpus} --format fastq --outdir ./ ${reads}
process
trimming
{
tag
"${reads}"
cpus
4
publishDir
"results/
fastq_SE
/trimming/"
,
mode:
'copy'
publishDir
"results/
SE_CCA_sequencing/fastq_SE_filtered
/trimming/"
,
mode:
'copy'
input:
file
reads
from
fastq_files_trim
...
...
@@ -142,7 +142,7 @@ fastq_files_urqt.into{fastq_files_CCA; fastq_files_urqt_fastq}
process
fastqc_fastq_urqt
{
tag
"$reads.baseName"
publishDir
"results/
fastq_SE
/fastqc/urqt/"
,
mode:
'copy'
publishDir
"results/
SE_CCA_sequencing/fastq_SE_filtered
/fastqc/urqt/"
,
mode:
'copy'
input:
file
reads
from
fastq_files_urqt_fastq
...
...
@@ -218,7 +218,7 @@ fi
process
mapping_fastq
{
tag
"$reads.baseName"
cpus
4
publishDir
"results/mapping_SE/bams/"
,
mode:
'copy'
publishDir
"results/
SE_CCA_sequencing/
mapping_SE
_CCA
/bams/"
,
mode:
'copy'
input:
file
reads
from
fastq_files_cut_CCA
...
...
@@ -270,7 +270,7 @@ samtools view -hb -q 2 ${bam} > ${bam}_filtered.bam
process
multiqc
{
tag
"$repport"
publishDir
"results/
fastq_SE/multiqc/
"
,
mode:
'copy'
publishDir
"results/
SE_CCA_sequencing/fastq_SE_filtered/multiqc/CCA_removal
"
,
mode:
'copy'
cpus
=
1
input:
...
...
@@ -292,7 +292,7 @@ multiqc -f .
process
sort_bam
{
tag
"$bam.baseName"
cpus
4
publishDir
"results/mapping_SE/bams/"
,
mode:
'copy'
publishDir
"results/
SE_CCA_sequencing/
mapping_SE
_CCA
/bams/"
,
mode:
'copy'
input:
file
bam
from
filtered_bam_files
...
...
@@ -308,7 +308,7 @@ samtools sort -@ ${task.cpus} -O BAM -o ${bam.baseName}_sorted.bam ${bam}
process
index_bam
{
tag
"$bam.baseName"
publishDir
"results/mapping_SE/bams/"
,
mode:
'copy'
publishDir
"results/
SE_CCA_sequencing/
mapping_SE
_CCA
/bams/"
,
mode:
'copy'
input:
file
bam
from
sorted_bam_files
output:
...
...
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