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mherbett
nextflow
Commits
48ca4a68
Commit
48ca4a68
authored
Jun 6, 2018
by
mherbett
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RNASeq.nf : removed co o sinle end triming
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7d39b772
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@@ -6,36 +6,6 @@
/* Illumina adaptor removal */
/*
* for paired-end data
*/
params
.
fastq
=
"$baseDir/data/fastq/*_{1,2}.fastq"
log
.
info
"fastq files : ${params.fastq}"
Channel
.
fromFilePairs
(
params
.
fastq
)
.
ifEmpty
{
error
"Cannot find any fastq files matching: ${params.fastq}"
}
.
set
{
fastq_files
}
process
adaptor_removal
{
tag
"$pair_id"
publishDir
"results/fastq/adaptor_removal/"
,
mode:
'copy'
input:
set
pair_id
,
file
(
reads
)
from
fastq_files
output:
file
"*_cut_R{1,2}.fastq.gz"
into
fastq_files_cut
script:
"""
cutadapt -a AGATCGGAAGAG -g CTCTTCCGATCT -A AGATCGGAAGAG -G CTCTTCCGATCT \
-o ${pair_id}_cut_R1.fastq.gz -p ${pair_id}_cut_R2.fastq.gz \
${reads[0]} ${reads[1]} > ${pair_id}_report.txt
"""
}
/*
* for single-end data
...
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