urqt.nf

/*
* urqt :
* Imputs : fastq files
* Output : fastq files
*/
/*                      quality trimming                                     */

/*
* for paired-end data
*/

params.fastq = "$baseDir/data/fastq/*_{1,2}.fastq"

log.info "fastq files : ${params.fastq}"

Channel
  .fromFilePairs( params.fastq )
  .ifEmpty { error "Cannot find any fastq files matching: ${params.fastq}" }
  .set { fastq_files }

process trimming {
  tag "$pair_id"
  cpus 4
  publishDir "results/fastq/trimming/", mode: 'copy'

  input:
  set pair_id, file(reads) from fastq_files

  output:
  file "*_trim_R{1,2}.fastq.gz" into fastq_files_cut

  script:
"""
UrQt --t 20 --m ${task.cpus} --gz \
--in ${reads[0]} --inpair ${reads[1]} \
--out ${pair_id}_trim_R1.fastq.gz --outpair ${pair_id}_trim_R2.fastq.gz \
> ${pair_id}_trimming_report.txt
"""
}

/*
* for single-end data
*/

params.fastq = "$baseDir/data/fastq/*.fastq"

log.info "fastq files : ${params.fastq}"

Channel
  .fromPath( params.fastq )
  .ifEmpty { error "Cannot find any fastq files matching: ${params.fastq}" }
  .set { fastq_files }

process trimming {
  tag "$reads.baseName"
  cpus 4
  publishDir "results/fastq/trimming/", mode: 'copy'

  input:
  file reads from fastq_files

  output:
  file "*_trim.fastq.gz" into fastq_files_trim

  script:
"""

UrQt --t 20 --m ${task.cpus} --gz \
--in ${reads} \
--out ${reads.baseName}_trim.fastq.gz \
> ${reads.baseName}_trimming_report.txt
"""
}