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Commit 2b2b50b9 authored by Xavier Grand's avatar Xavier Grand
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add STAR and HTseq to RNAseq_XGR.nf

parent 85e66de8
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...@@ -76,6 +76,7 @@ params.star_index_out = "$params.project/STARindex/" ...@@ -76,6 +76,7 @@ params.star_index_out = "$params.project/STARindex/"
log.info "Annotation: ${params.gtf}" log.info "Annotation: ${params.gtf}"
log.info "Genome fasta file: ${params.fasta}" log.info "Genome fasta file: ${params.fasta}"
log.info "Genome index location: ${params.idx}"
/* /*
**************************************************************** ****************************************************************
...@@ -104,6 +105,7 @@ include { multiqc } from './nf_modules/multiqc/main.nf' addParams(multiqc_out: " ...@@ -104,6 +105,7 @@ include { multiqc } from './nf_modules/multiqc/main.nf' addParams(multiqc_out: "
include { fastp } from "./nf_modules/fastp/main.nf" include { fastp } from "./nf_modules/fastp/main.nf"
include { index_with_gtf } from "./nf_modules/star/main_2.7.8a.nf" include { index_with_gtf } from "./nf_modules/star/main_2.7.8a.nf"
include { mapping_fastq } from "./nf_modules/star/main_2.7.8a.nf" include { mapping_fastq } from "./nf_modules/star/main_2.7.8a.nf"
include { mapping_withindex } from "./nf_modules/star/main_2.7.8a.nf"
include { htseq_count } from "./nf_modules/htseq/main.nf" include { htseq_count } from "./nf_modules/htseq/main.nf"
/* /*
...@@ -150,7 +152,7 @@ workflow { ...@@ -150,7 +152,7 @@ workflow {
.fromPath( idx_genome ) .fromPath( idx_genome )
.ifEmpty { error "Cannot find idexed genome reference files" } .ifEmpty { error "Cannot find idexed genome reference files" }
.set { genome_indexed_input } .set { genome_indexed_input }
mapping_fastq(genome_indexed_input, fastp.out.fastq) mapping_withindex(genome_indexed_input, fastp.out.fastq)
} }
//######################## HTseq COUNT ######################### //######################## HTseq COUNT #########################
......
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