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LBMC
nextflow
Commits
1207cdab
Verified
Commit
1207cdab
authored
4 years ago
by
Laurent Modolo
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gatk3: add new processes
parent
eddc374c
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src/nf_modules/gatk3/main.nf
+164
-12
164 additions, 12 deletions
src/nf_modules/gatk3/main.nf
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164 additions
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12 deletions
src/nf_modules/gatk3/main.nf
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12
View file @
1207cdab
...
...
@@ -24,11 +24,11 @@ gatk3 -T HaplotypeCaller \
process filter_snp {
container = "${container_url}"
label "big_mem_m
ono
_cpus"
label "big_mem_m
ulti
_cpus"
tag "$file_id"
input:
tuple val(file_id), path(v
ariants
)
tuple val(file_id), path(v
cf
)
tuple val(ref_id), path(fasta), path(fai), path(dict)
output:
tuple val(file_id), path("*_snp.vcf"), emit: vcf
...
...
@@ -37,7 +37,7 @@ process filter_snp {
gatk3 -T SelectVariants \
-nct ${task.cpus} \
-R ${fasta} \
-V ${v
ariants
} \
-V ${v
cf
} \
-selectType SNP \
-o ${file_id}_snp.vcf
"""
...
...
@@ -45,11 +45,11 @@ gatk3 -T SelectVariants \
process filter_indels {
container = "${container_url}"
label "big_mem_m
ono
_cpus"
label "big_mem_m
ulti
_cpus"
tag "$file_id"
input:
tuple val(file_id), path(v
ariants
)
tuple val(file_id), path(v
cf
)
tuple val(ref_id), path(fasta), path(fai), path(dict)
output:
tuple val(file_id), path("*_indel.vcf"), emit: vcf
...
...
@@ -58,7 +58,7 @@ process filter_indels {
gatk3 -T SelectVariants \
-nct ${task.cpus} \
-R ${fasta} \
-V ${v
ariants
} \
-V ${v
cf
} \
-selectType INDEL \
-o ${file_id}_indel.vcf
"""
...
...
@@ -68,11 +68,11 @@ high_confidence_snp_filter = "(QD < 2.0) || (FS > 60.0) || (MQ < 40.0) || (MQRan
process high_confidence_snp {
container = "${container_url}"
label "big_mem_m
ono
_cpus"
label "big_mem_m
ulti
_cpus"
tag "$file_id"
input:
tuple val(file_id), path(v
ariants
)
tuple val(file_id), path(v
cf
)
tuple val(ref_id), path(fasta), path(fai), path(dict)
output:
tuple val(file_id), path("*_snp.vcf"), emit: vcf
...
...
@@ -81,7 +81,7 @@ process high_confidence_snp {
gatk3 -T VariantFiltration \
-nct ${task.cpus} \
-R ${fasta} \
-V ${v
ariants
} \
-V ${v
cf
} \
--filterExpression "${high_confidence_snp_filter}" \
--filterName "basic_snp_filter" \
-o ${file_id}_filtered_snp.vcf
...
...
@@ -92,11 +92,11 @@ high_confidence_indel_filter = "QD < 3.0 || FS > 200.0 || ReadPosRankSum < -20.0
process high_confidence_indels {
container = "${container_url}"
label "big_mem_m
ono
_cpus"
label "big_mem_m
ulti
_cpus"
tag "$file_id"
input:
tuple val(file_id), path(v
ariants
)
tuple val(file_id), path(v
cf
)
tuple val(ref_id), path(fasta), path(fai), path(dict)
output:
tuple val(file_id), path("*_indel.vcf"), emit: vcf
...
...
@@ -105,9 +105,161 @@ process high_confidence_indels {
gatk3 -T VariantFiltration \
-nct ${task.cpus} \
-R ${fasta} \
-V ${v
ariants
} \
-V ${v
cf
} \
--filterExpression "${high_confidence_indel_filter}" \
--filterName "basic_indel_filter" \
-o ${file_id}_filtered_indel.vcf
"""
}
process recalibrate_snp_table {
container = "${container_url}"
label "big_mem_multi_cpus"
tag "$file_id"
input:
tuple val(file_id), path(snp_file), path(indel_file), path(bam), path(bam_idx)
tuple val(ref_id), path(fasta), path(fai), path(dict)
output:
tuple val(file_id), path("recal_data_table"), emit: recal_table
script:
"""
gatk3 -T BaseRecalibrator \
-nct ${task.cpus} \
-R ${fasta} \
-I ${bam} \
-knownSites ${snp_file} \
-knownSites ${indel_file} \
-o recal_data_table
"""
}
process recalibrate_snp {
container = "${container_url}"
label "big_mem_multi_cpus"
tag "$file_id"
input:
tuple val(file_id), path(snp_file), path(indel_file), path(bam), path(bam_idx)
tuple val(table_id), path(recal_data_table)
tuple val(ref_id), path(fasta), path(fai), path(dict)
output:
tuple val(file_id), path("*.bam"), emit: bam
script:
"""
gatk3 -T PrintReads \
--use_jdk_deflater \
--use_jdk_inflater \
-nct ${task.cpus} \
-R ${fasta} \
-I ${bam} \
-BQSR recal_data_table \
-o ${file_id}_recal.bam
"""
}
process haplotype_caller {
container = "${container_url}"
label "big_mem_multi_cpus"
tag "$file_id"
input:
tuple val(file_id), path(bam)
tuple val(ref_id), path(fasta), path(fai), path(dict)
output:
tuple val(file_id), path("*.gvcf"), emit: gvcf
script:
"""
gatk3 -T HaplotypeCaller \
-nct ${task.cpus} \
-R ${fasta} \
-I ${bam} \
-ERC GVCF \
-variant_index_type LINEAR -variant_index_parameter 128000 \
-o ${file_id}.gvcf
"""
}
process gvcf_genotyping {
container = "${container_url}"
label "big_mem_multi_cpus"
tag "$file_id"
input:
tuple val(file_id), path(gvcf)
tuple val(ref_id), path(fasta), path(fai), path(dict)
output:
tuple val(file_id), path("*.vcf"), emit: vcf
script:
"""
gatk3 -T GenotypeGVCFs \
-nct ${task.cpus} \
-R ${fasta} \
-V ${gvcf} \
-o ${file_id}_joint.vcf
"""
}
process select_variants_snp {
container = "${container_url}"
label "big_mem_multi_cpus"
tag "$file_id"
input:
tuple val(file_id), path(vcf)
tuple val(ref_id), path(fasta), path(fai), path(dict)
output:
tuple val(file_id), path("*_joint_snp.vcf"), emit: vcf
script:
"""
gatk3 -T SelectVariants \
-nct ${task.cpus} \
-R ${fasta} \
-V ${vcf} \
-selectType SNP \
-o ${file_id}_joint_snp.vcf
"""
}
process select_variants_indels {
container = "${container_url}"
label "big_mem_multi_cpus"
tag "$file_id"
input:
tuple val(file_id), path(vcf)
tuple val(ref_id), path(fasta), path(fai), path(dict)
output:
tuple val(file_id), path("*_joint_indel.vcf"), emit: vcf
script:
"""
gatk3 -T SelectVariants \
-nct ${task.cpus} \
-R ${fasta} \
-V ${vcf} \
-selectType INDEL \
-o ${file_id}_joint_indel.vcf
"""
}
process personalized_genome {
container = "${container_url}"
label "big_mem_mono_cpus"
tag "$file_id"
input:
tuple val(file_id), path(vcf)
tuple val(ref_id), path(fasta), path(fai), path(dict)
output:
tuple val(file_id), path("*_genome.fasta"), emit: fasta
script:
library = pick_library(file_id, library_list)
"""
gatk3 -T FastaAlternateReferenceMaker\
-R ${reference} \
-V ${vcf} \
-o ${library}_genome.fasta
"""
}
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