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LBMC
Hub
hic
Commits
8901a8e3
Commit
8901a8e3
authored
4 years ago
by
nservant
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[MODIF] edit README
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8901a8e3
...
@@ -23,8 +23,10 @@ well as protocols that do not require restriction enzymes such as DNase Hi-C.
...
@@ -23,8 +23,10 @@ well as protocols that do not require restriction enzymes such as DNase Hi-C.
In practice, this workflow was successfully applied to many data-sets including
In practice, this workflow was successfully applied to many data-sets including
dilution Hi-C, in situ Hi-C, DNase Hi-C, Micro-C, capture-C, capture Hi-C or
dilution Hi-C, in situ Hi-C, DNase Hi-C, Micro-C, capture-C, capture Hi-C or
HiChip data.
HiChip data.
Contact maps are generated in standard format including HiC-Pro, cooler, and h5 format.
Addition downstream analysis steps such as TADs calling are also available.
Contact maps are generated in standard format including HiC-Pro, cooler, and h5 format for
downstream analysis and visualization.
Addition analysis steps such as TADs calling are also available.
The pipeline is built using
[
Nextflow
](
https://www.nextflow.io
)
, a workflow tool
The pipeline is built using
[
Nextflow
](
https://www.nextflow.io
)
, a workflow tool
to run tasks across multiple compute infrastructures in a very portable manner.
to run tasks across multiple compute infrastructures in a very portable manner.
...
@@ -39,9 +41,10 @@ sites ([`bowtie2`](http://bowtie-bio.sourceforge.net/bowtie2/index.shtml))
...
@@ -39,9 +41,10 @@ sites ([`bowtie2`](http://bowtie-bio.sourceforge.net/bowtie2/index.shtml))
3.
Duplicates removal
3.
Duplicates removal
4.
Create genome-wide contact maps at various resolution (
[
`cooler`
](
https://github.com/open2c/cooler
)
)
4.
Create genome-wide contact maps at various resolution (
[
`cooler`
](
https://github.com/open2c/cooler
)
)
5.
Contact maps normalization using the ICE algorithm (
[
`cooler`
](
https://github.com/open2c/cooler
)
)
5.
Contact maps normalization using the ICE algorithm (
[
`cooler`
](
https://github.com/open2c/cooler
)
)
6.
Quality controls (
[
`HiC-Pro`
](
https://github.com/nservant/HiC-Pro
)
,
[
`HiCExplorer`
](
https://github.com/deeptools/HiCExplorer
)
)
6.
Export to various contact maps formats (
[
`HiC-Pro`
](
https://github.com/nservant/HiC-Pro
)
,
[
`cooler`
](
https://github.com/open2c/cooler
)
,
[
`HiCExplorer`
](
https://github.com/deeptools/HiCExplorer
)
)
7.
TADs calling (
[
`HiCExplorer`
](
https://github.com/deeptools/HiCExplorer
)
,
[
`cooler`
](
https://github.com/open2c/cooler
)
)
7.
Quality controls (
[
`HiC-Pro`
](
https://github.com/nservant/HiC-Pro
)
,
[
`HiCExplorer`
](
https://github.com/deeptools/HiCExplorer
)
)
8.
Quality control report (
[
`MultiQC`
](
https://multiqc.info/
)
)
8.
TADs calling (
[
`HiCExplorer`
](
https://github.com/deeptools/HiCExplorer
)
,
[
`cooler`
](
https://github.com/open2c/cooler
)
)
9.
Quality control report (
[
`MultiQC`
](
https://multiqc.info/
)
)
## Quick Start
## Quick Start
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