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Commit 8901a8e3 authored by nservant's avatar nservant
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[MODIF] edit README

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...@@ -23,8 +23,10 @@ well as protocols that do not require restriction enzymes such as DNase Hi-C. ...@@ -23,8 +23,10 @@ well as protocols that do not require restriction enzymes such as DNase Hi-C.
In practice, this workflow was successfully applied to many data-sets including In practice, this workflow was successfully applied to many data-sets including
dilution Hi-C, in situ Hi-C, DNase Hi-C, Micro-C, capture-C, capture Hi-C or dilution Hi-C, in situ Hi-C, DNase Hi-C, Micro-C, capture-C, capture Hi-C or
HiChip data. HiChip data.
Contact maps are generated in standard format including HiC-Pro, cooler, and h5 format.
Addition downstream analysis steps such as TADs calling are also available. Contact maps are generated in standard format including HiC-Pro, cooler, and h5 format for
downstream analysis and visualization.
Addition analysis steps such as TADs calling are also available.
The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool
to run tasks across multiple compute infrastructures in a very portable manner. to run tasks across multiple compute infrastructures in a very portable manner.
...@@ -39,9 +41,10 @@ sites ([`bowtie2`](http://bowtie-bio.sourceforge.net/bowtie2/index.shtml)) ...@@ -39,9 +41,10 @@ sites ([`bowtie2`](http://bowtie-bio.sourceforge.net/bowtie2/index.shtml))
3. Duplicates removal 3. Duplicates removal
4. Create genome-wide contact maps at various resolution ([`cooler`](https://github.com/open2c/cooler)) 4. Create genome-wide contact maps at various resolution ([`cooler`](https://github.com/open2c/cooler))
5. Contact maps normalization using the ICE algorithm ([`cooler`](https://github.com/open2c/cooler)) 5. Contact maps normalization using the ICE algorithm ([`cooler`](https://github.com/open2c/cooler))
6. Quality controls ([`HiC-Pro`](https://github.com/nservant/HiC-Pro), [`HiCExplorer`](https://github.com/deeptools/HiCExplorer)) 6. Export to various contact maps formats ([`HiC-Pro`](https://github.com/nservant/HiC-Pro), [`cooler`](https://github.com/open2c/cooler), [`HiCExplorer`](https://github.com/deeptools/HiCExplorer))
7. TADs calling ([`HiCExplorer`](https://github.com/deeptools/HiCExplorer), [`cooler`](https://github.com/open2c/cooler)) 7. Quality controls ([`HiC-Pro`](https://github.com/nservant/HiC-Pro), [`HiCExplorer`](https://github.com/deeptools/HiCExplorer))
8. Quality control report ([`MultiQC`](https://multiqc.info/)) 8. TADs calling ([`HiCExplorer`](https://github.com/deeptools/HiCExplorer), [`cooler`](https://github.com/open2c/cooler))
9. Quality control report ([`MultiQC`](https://multiqc.info/))
## Quick Start ## Quick Start
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