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LBMC
Hub
hic
Commits
804cd499
Commit
804cd499
authored
5 years ago
by
nservant
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update igenomes
parent
c93fa3be
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3 changed files
conf/hicpro.config
+4
-4
4 additions, 4 deletions
conf/hicpro.config
conf/test.config
+1
-1
1 addition, 1 deletion
conf/test.config
main.nf
+5
-4
5 additions, 4 deletions
main.nf
with
10 additions
and
9 deletions
conf/hicpro.config
+
4
−
4
View file @
804cd499
...
...
@@ -9,9 +9,9 @@
params
{
//
Genome
Reference
bwt2_index
=
chromosome_size
=
//
Reference
chromosome_size
=
false
bwt2_index
=
false
//
Alignment
options
bwt2_opts_end2end
=
'--very-sensitive -L 30 --score-min L,-0.6,-0.2 --end-to-end --reorder'
...
...
@@ -20,7 +20,7 @@ params {
//
Digestion
Hi
-
C
restriction_site
=
'A^AGGCT'
restriction_fragments
=
restriction
g
_fragments
=
false
ligation_site
=
'AAGCTAGCTT'
min_restriction_fragment_size
=
0
max_restriction_fragment_size
=
100
...
...
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conf/test.config
+
1
−
1
View file @
804cd499
...
...
@@ -24,5 +24,5 @@ params {
]
//
Annotations
genome
=
'GR
c
h37'
genome
=
'GR
C
h37'
}
This diff is collapsed.
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main.nf
+
5
−
4
View file @
804cd499
...
...
@@ -49,15 +49,15 @@ def helpMessage() {
--bwt2_index Path to Bowtie2 index
--fasta Path to Fasta reference
--chromosome_size Path to chromosome size file
--restriction_fragment
_bed
Path to restriction fragment file (bed)
--restriction_fragment
s
Path to restriction fragment file (bed)
Options:
--bwt2_opts_end2end Options for bowtie2 end-to-end mappinf (first mapping step)
--bwt2_opts_trimmed Options for bowtie2 mapping after ligation site trimming
--min_mapq Minimum mapping quality values to consider
--restriction
-
site Cutting motif(s) of restriction enzyme(s) (comma separated)
--ligation
-
site Ligation motifs to trim (comma separated)
--restriction
_
site Cutting motif(s) of restriction enzyme(s) (comma separated)
--ligation
_
site Ligation motifs to trim (comma separated)
--min_restriction_fragment_size Minimum size of restriction fragments to consider
--max_restriction_framgnet_size Maximum size of restriction fragmants to consider
...
...
@@ -210,6 +210,7 @@ else if ( params.fasta && params.restriction_site ){
Channel.fromPath(params.fasta)
.ifEmpty { exit 1, "Fasta file not found: ${params.fasta}" }
.set { fasta_for_resfrag }
}
else {
exit 1, "No restriction fragments file specified!"
}
...
...
@@ -344,7 +345,7 @@ if(!params.chromosome_size && params.fasta){
}
}
if(!params.
bwt2_index
&& params.fasta){
if(!params.
restriction_fragments
&& params.fasta){
process makeRestrictionFragments {
tag "$fasta"
publishDir path: { params.saveReference ? "${params.outdir}/reference_genome" : params.outdir },
...
...
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