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LBMC
Palladino
RNAseq_nextflow
Commits
8e91baf6
Commit
8e91baf6
authored
3 years ago
by
Carine Rey
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change gff fro gtf
parent
366a0d55
Branches
Branches containing commit
Tags
v0.2.4
Tags containing commit
1 merge request
!3
change gff for gtf
Changes
1
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1 changed file
src/RNAseq_cec.nf
+10
-9
10 additions, 9 deletions
src/RNAseq_cec.nf
with
10 additions
and
9 deletions
src/RNAseq_cec.nf
+
10
−
9
View file @
8e91baf6
...
@@ -3,7 +3,8 @@ nextflow.enable.dsl=2
...
@@ -3,7 +3,8 @@ nextflow.enable.dsl=2
log
.
info
"fastq files: ${params.fastq}"
log
.
info
"fastq files: ${params.fastq}"
log
.
info
"fasta cds file: ${params.cds_fasta}"
log
.
info
"fasta cds file: ${params.cds_fasta}"
log
.
info
"fasta genomic file: ${params.genomic_fasta}"
log
.
info
"fasta genomic file: ${params.genomic_fasta}"
log
.
info
"fasta gff3 file: ${params.genomic_gff3}"
log
.
info
"fasta gtf file: ${params.genomic_gtf}"
include
{
fastp
}
from
'./nf_modules/fastp/main.nf'
addParams
(
fastp_out:
"fastQC/"
)
include
{
fastp
}
from
'./nf_modules/fastp/main.nf'
addParams
(
fastp_out:
"fastQC/"
)
include
{
include
{
...
@@ -20,7 +21,7 @@ include {
...
@@ -20,7 +21,7 @@ include {
)
)
include
{
include
{
index_with_g
f
f
as
star_index_with_g
f
f
;
index_with_g
t
f
as
star_index_with_g
t
f
;
mapping_fastq
as
star_mapping_fastq
mapping_fastq
as
star_mapping_fastq
}
from
'./nf_modules/star/main.nf'
addParams
(
}
from
'./nf_modules/star/main.nf'
addParams
(
star_mapping_fastq_out:
"star_bam/"
star_mapping_fastq_out:
"star_bam/"
...
@@ -31,7 +32,7 @@ include { index_bam
...
@@ -31,7 +32,7 @@ include { index_bam
htseq_out:
"star_bam/"
htseq_out:
"star_bam/"
)
)
include
{
htseq_count_with_g
f
f
include
{
htseq_count
as
htseq_count_with_g
t
f
}
from
'./nf_modules/htseq/main.nf'
addParams
(
}
from
'./nf_modules/htseq/main.nf'
addParams
(
htseq_out:
"htseq_count/"
htseq_out:
"htseq_count/"
)
)
...
@@ -61,10 +62,10 @@ channel
...
@@ -61,10 +62,10 @@ channel
.
set
{
genomic_fasta_file
}
.
set
{
genomic_fasta_file
}
channel
channel
.
fromPath
(
params
.
genomic_gf
f3
)
.
fromPath
(
params
.
genomic_g
t
f
)
.
ifEmpty
{
error
"Cannot find any gf
f3
files matching: ${params.genomic_gf
f3
}"
}
.
ifEmpty
{
error
"Cannot find any g
t
f files matching: ${params.genomic_g
t
f}"
}
.
map
{
it
->
[
it
.
simpleName
,
it
]}
.
map
{
it
->
[
it
.
simpleName
,
it
]}
.
set
{
genome_gf
f3
_file
}
.
set
{
genome_g
t
f_file
}
workflow
{
workflow
{
//trim data
//trim data
...
@@ -82,7 +83,7 @@ workflow {
...
@@ -82,7 +83,7 @@ workflow {
//star mapping
//star mapping
//// star index
//// star index
star_index_with_g
f
f
(
genomic_fasta_file
,
genome_gf
f3
_file
)
star_index_with_g
t
f
(
genomic_fasta_file
,
genome_g
t
f_file
)
star_index
=
star_index_with_gff
.
out
star_index
=
star_index_with_gff
.
out
////star mapping
////star mapping
...
@@ -95,9 +96,9 @@ workflow {
...
@@ -95,9 +96,9 @@ workflow {
//// index bam
//// index bam
index_bam
(
star_mapping_fastq
.
out
.
bam
)
index_bam
(
star_mapping_fastq
.
out
.
bam
)
htseq_count_with_g
f
f
(
htseq_count_with_g
t
f
(
index_bam
.
out
.
bam_idx
,
index_bam
.
out
.
bam_idx
,
genome_gf
f3
_file
genome_g
t
f_file
)
)
//report multiqc
//report multiqc
...
...
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