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SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
SPDX-License-Identifier: CC-BY-SA-4.0
[Formation BioComp] nextflow for experimental biologists
Dear all,
You registered for the nextflow formation which will take place this afternoon starting at 13h30 in the room M7.1H04.
Please check that you have an account on our gitlab server at the following url :
https://gitlab.biologie.ens-lyon.fr
To connect, simply click on the CAS button.
Your account is now linked to the gitlab server, but blocked
You can send an email to laurent.modolo@ens-lyon.fr to activate your account.
You will also need an account on the PSMN (http://www.ens-lyon.fr/PSMN/doku.php?id=contact:forms:inscription)
If you want to work on your laptop, or plan to work remotely on your desktop via ssh (http://www.ens-lyon.fr/LBMC/intranet/services-communs/pole-bioinformatique/bioinfo_club/1_ssh_and_other_tools_to_work_remotly) you need to have the following software installed :
- ssh
- git
- java (>=1.8)
- docker
Best,
Dear all,
You registered to the formation Git (12/12) or/and the formation Nextlflow (13/12) from 13:30 pm to 18pm in the in the room M7.1H04.
Please check that you have an account on our gitlab server at the following url :
https://gitlab.biologie.ens-lyon.fr (only available from the ENS network)
To connect, simply click on the CAS button.
Your account is now linked to the gitlab server, but blocked
You can send an email to laurent.modolo@ens-lyon.fr to activate your account.
You will also need an account on the PSMN (http://www.ens-lyon.fr/PSMN/doku.php?id=contact:forms:inscription) (don’t wait until the 12 to request an account !)
If you want to work on your laptop, or plan to work remotely on your desktop via ssh (http://www.ens-lyon.fr/LBMC/intranet/services-communs/pole-bioinformatique/bioinfo_club/1_ssh_and_other_tools_to_work_remotly) you need to have the following software installed :
- ssh
- git
- java (>=1.8) (for the nextflow formation)
- docker (for the nextflow formation)
Otherwise, you will need to request an account to use the computer in the room (http://www.cbp.ens-lyon.fr/doku.php?id=contact:compte)
Best,
SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
SPDX-License-Identifier: CC-BY-SA-4.0
<!--
SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
SPDX-License-Identifier: CC-BY-SA-4.0
-->
## Projects using nextflow
This page list existing Nextflow based pipeline projects into different categories
- [RNASeq](./nf_projects.md#rnaseq)
- [scRNASeq](./nf_projects.m#scrnaseq)
- [DNASeq](./nf_projects.md#dnaseq)
- [ChipSeq](./nf_projects.md#chipseq)
To add your project to this list, please fork this project, modify this file and make a merge request.
![merge request button](./img/merge_request.png)
### RNASeq
- [https://_https://gitlab.biologie.ens-lyon.fr/gylab/salmoninyeast](https://_https://gitlab.biologie.ens-lyon.fr/gylab/salmoninyeast)
- [https://github.com/LBMC/readthroughpombe](https://github.com/LBMC/readthroughpombe)
- [https://_https://gitlab.biologie.ens-lyon.fr/vvanoost/nextflow](https://_https://gitlab.biologie.ens-lyon.fr/vvanoost/nextflow)
- [https://gitlab.biologie.ens-lyon.fr/elabaron/HIV_project](https://gitlab.biologie.ens-lyon.fr/elabaron/HIV_project)
- [https://gitbio.ens-lyon.fr/LBMC/Palladino/RNAseq_nextflow](https://gitbio.ens-lyon.fr/LBMC/Palladino/RNAseq_nextflow)
### scRNASeq
- [https://gitlab.com/LBMC_UMR5239/sbdm/mars-seq](https://gitlab.com/LBMC_UMR5239/sbdm/mars-seq)
### DNASeq
- [https://github.com/LBMC/ChrSexebelari](https://github.com/LBMC/ChrSexebelari)
- [https://gitbio.ens-lyon.fr/LBMC/gylab/MappingNoise](https://gitbio.ens-lyon.fr/LBMC/gylab/MappingNoise)
- [https://gitbio.ens-lyon.fr/LBMC/qrg/droso_hic_group/droso_haplo_rna_seq](https://gitbio.ens-lyon.fr/LBMC/qrg/droso_hic_group/droso_haplo_rna_seq)
### Chip-Seq
- [https://gitlab.biologie.ens-lyon.fr/Auboeuf/ChIP-seq](https://gitlab.biologie.ens-lyon.fr/Auboeuf/ChIP-seq)
- [https://gitbio.ens-lyon.fr/LBMC/Bernard/quantitative-nucleosome-analysis](https://gitbio.ens-lyon.fr/LBMC/Bernard/quantitative-nucleosome-analysis)
# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later
* *
# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later
FROM ubuntu:22.04
ENV CITESEQ_VERSION=1.4.5
ENV PACKAGES procps locales gcc libstdc++-10-dev libcurl4 libc6 python3 python3-pip python3-dev
RUN apt-get update && \
apt-get install -y --no-install-recommends ${PACKAGES} && \
apt-get clean
RUN locale-gen en_US.UTF-8
ENV LC_ALL=en_US.utf-8
ENV LANG=en_US.utf-8
ENV LC_ALL=C.UTF-8
ENV LANG=C.UTF-8
RUN pip3 install CITE-seq-Count==${CITESEQ_VERSION}
#!/bin/sh
# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later
docker pull lbmc/cite_seq_count:1.4.5
docker build src/.docker_modules/CITE-seq-Count/1.4.5 -t 'lbmc/cite_seq_count:1.4.5'
docker push lbmc/cite_seq_count:1.4.5
# docker buildx build --platform linux/amd64,linux/arm64 -t "lbmc/cite_seq_count:1.4.5" --push src/.docker_modules/CITE-seq-Count/1.4.5
# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later
FROM ubuntu:22.04
ENV PACKAGES procps locales gcc libstdc++-10-dev libcurl4 libc6 python3 python3-pip python3-dev git
RUN apt-get update && \
apt-get install -y --no-install-recommends ${PACKAGES} && \
apt-get clean
RUN locale-gen en_US.UTF-8
ENV LC_ALL=en_US.utf-8
ENV LANG=en_US.utf-8
ENV LC_ALL=C.UTF-8
ENV LANG=C.UTF-8
RUN git clone https://github.com/Hoohm/CITE-seq-Count.git \
&& cd CITE-seq-Count \
&& git checkout 1.5.0-alpha \
&& pip3 install requests pooch \
&& pip3 install ./
#!/bin/sh
# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later
docker pull lbmc/cite_seq_count:1.5.0
docker build src/.docker_modules/CITE-seq-Count/1.5.0 -t 'lbmc/cite_seq_count:1.5.0'
docker push lbmc/cite_seq_count:1.5.0
# docker buildx build --platform linux/amd64,linux/arm64 -t "lbmc/cite_seq_count:1.5.0" --push src/.docker_modules/CITE-seq-Count/1.5.0
# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later
FROM quay.io/biocontainers/agat:0.8.0--pl5262hdfd78af_0
\ No newline at end of file
#!/bin/sh
# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later
docker pull lbmc/agat:0.8.0
# docker build src/.docker_modules/agat/0.8.0 -t 'lbmc/agat:0.8.0'
# docker push lbmc/agat:0.8.0
docker buildx build --platform linux/amd64,linux/arm64 -t "lbmc/agat:0.8.0" --push src/.docker_modules/agat/0.8.0
# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later
FROM python:3.9-buster as build
MAINTAINER Laurent Modolo
ENV ALNTOOLS_VERSION=dd96682
ENV PACKAGES git \
ca-certificates \
gawk \
procps
RUN apt-get update \
&& apt-get install -y --no-install-recommends ${PACKAGES}\
&& apt-get clean \
&& git clone https://github.com/churchill-lab/alntools.git \
&& cd alntools \
&& git checkout ${ALNTOOLS_VERSION} \
&& python setup.py install \
&& cd .. \
&& rm -R alntools \
&& pip install six \
&& apt-get autoremove --purge -y git ca-certificates
CMD ["bash"]
\ No newline at end of file
#!/bin/sh
# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later
docker pull lbmc/alntools:dd96682
# docker build src/.docker_modules/alntools/dd96682 -t 'lbmc/alntools:dd96682'
# docker push lbmc/alntools:dd96682
docker buildx build --platform linux/amd64,linux/arm64 -t "lbmc/alntools:dd96682" --push src/.docker_modules/alntools/dd96682
# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later
FROM ubuntu:18.04
MAINTAINER Laurent Modolo
ENV BAMUTILS_VERSION=1.0.14
ENV PACKAGES git \
build-essential \
g++ \
libssl-dev \
zlib1g-dev
RUN apt-get update && apt-get -y install ${PACKAGES}
RUN git clone https://github.com/statgen/libStatGen && \
cd libStatGen && \
git checkout fae4fca874b3b78bf9b61c0 && \
make && \
cd ../ && \
git clone https://github.com/statgen/bamUtil && \
cd bamUtil && \
git checkout v${BAMUTILS_VERSION} && \
make && \
make install
#!/bin/sh
# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later
docker pull lbmc/bamutils:1.0.14
# docker build src/.docker_modules/bamutils/1.0.14 -t 'lbmc/bamutils:1.0.14'
# docker push lbmc/bamutils:1.0.14
docker buildx build --platform linux/amd64,linux/arm64 -t "lbmc/bamutils:1.0.14" --push src/.docker_modules/bamutils/1.0.14
# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later
FROM ubuntu:18.04 FROM ubuntu:18.04
MAINTAINER Laurent Modolo MAINTAINER Laurent Modolo
ENV SAMTOOLS_VERSION=1.7 ENV BCFTOOLS_VERSION=1.7
ENV PACKAGES samtools=${SAMTOOLS_VERSION}* ENV PACKAGES bcftools=${BCFTOOLS_VERSION}*
RUN apt-get update && \ RUN apt-get update && \
apt-get install -y --no-install-recommends ${PACKAGES} && \ apt-get install -y --no-install-recommends ${PACKAGES} && \
......
#!/bin/sh
# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later
docker pull lbmc/bcftools:1.7
# docker build src/.docker_modules/bcftools/1.7 -t 'lbmc/bcftools:1.7'
# docker push lbmc/bcftools:1.7
docker buildx build --platform linux/amd64,linux/arm64 -t "lbmc/bcftools:1.7" --push src/.docker_modules/bcftools/1.7
# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later
FROM quay.io/biocontainers/bedops:2.4.39--hc9558a2_0
MAINTAINER Laurent Modolo
#!/bin/sh
# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later
docker pull lbmc/bedops:2.4.39
# docker build src/.docker_modules/bedops/2.4.39 -t 'lbmc/bedops:2.4.39'
# docker push lbmc/bedops:2.4.39
docker buildx build --platform linux/amd64,linux/arm64 -t "lbmc/bedops:2.4.39" --push src/.docker_modules/bedops/2.4.39
# SPDX-FileCopyrightText: 2022 Laurent Modolo <laurent.modolo@ens-lyon.fr>
#
# SPDX-License-Identifier: AGPL-3.0-or-later
FROM ubuntu:16.04 FROM ubuntu:16.04
MAINTAINER Laurent Modolo MAINTAINER Laurent Modolo
......