diff --git a/src/bolero.nf b/src/bolero.nf index 163eed6fafd206f59e04e7676a3d0d18dfa71296..0e479da428d588f4b1e72ab1c36057596dcb10f9 100755 --- a/src/bolero.nf +++ b/src/bolero.nf @@ -92,6 +92,8 @@ params.num_callers = 16 params.chunks_per_runner = 512 params.chunk_size = 1900 params.config_file = "" +params.kit_barcoding = "EXP-PBC001" + /* Params out */ @@ -104,7 +106,6 @@ params.minimap2_genome_out = "05_minimap2/" params.start_position_counts_out = "06_start_positions/" params.nanosplicer_out = "07_nanosplicer/" params.rna_count_out = "08_RNA_count/" -params.pycoQC_out = "pycoQC/" /* **************************************************************** @@ -156,6 +157,7 @@ Channel .map(it -> [it.baseName, it]) .set{barcodes} + // Test pour barcoding process Channel .fromPath(params.pass) @@ -169,15 +171,17 @@ Channel if(!params.skipBC) { /* Hardware configuration, if Nvidia CUDA compatible graphic card is installed, use guppy-gpu, else guppy-cpu (much slower)*/ - include { barcoding } from "./nf_modules/ont-guppy/main.nf" if(params.gpu_mode) { include { basecall_fast5_gpu } from "./nf_modules/ont-guppy/main.nf" + include { barcoding_gpu } from "./nf_modules/ont-guppy/main.nf" } else { include { basecall_fast5_cpu } from "./nf_modules/ont-guppy/main.nf" + include { barcoding_cpu } from "./nf_modules/ont-guppy/main.nf" } } +<<<<<<< HEAD <<<<<<< HEAD <<<<<<< HEAD // include { barecode } from "./nf_modules/barecode/main.nf" @@ -186,6 +190,10 @@ if(!params.skipBC) { ======= include { pycoqc } from "./nf_modules/pycoqc/main.nf" >>>>>>> 9ef97be (Contrôle qualité et basecalling en mode gpu) +======= +include { barcoding_cpu } from "./nf_modules/ont-guppy/main.nf" +include { control_basecalling } from "./nf_modules/pycoqc/main.nf" +>>>>>>> 8667704 (Ajout ded l'option barcode aux scripts R) include { concatenate } from "./nf_modules/seqkit/main.nf" include { cut_5pRACE } from "./nf_modules/cutadapt/main.nf" include { hbv_genome } from "./nf_modules/minimap2/main.nf" @@ -208,7 +216,7 @@ include { rna_count } from "./nf_modules/rna_count/main.nf" workflow { pass.view() - barcoding(pass) + barcoding_cpu(pass) //######################## BASECALLING ######################## /* @@ -221,12 +229,12 @@ workflow { if(params.gpu_mode) { basecall_fast5_gpu(input) concatenate(basecall_fast5_gpu.out.pass) - pycoqc(basecall_fast5_gpu.sequencing_summary) + control_basecalling(basecall_fast5_gpu.sequencing_summary) } else { basecall_fast5_cpu(input) concatenate(basecall_fast5_cpu.out.pass) - pycoqc(basecall_fast5_cpu.sequencing_summary) + control_basecalling(basecall_fast5_cpu.sequencing_summary) } } diff --git a/src/nf_modules/junction_nanosplicer/main.nf b/src/nf_modules/junction_nanosplicer/main.nf index fb391396b8bee9ee39bee4a80818d7db4947e586..a48c713e70903f8f604bfa59e5b3ffabbca6737f 100644 --- a/src/nf_modules/junction_nanosplicer/main.nf +++ b/src/nf_modules/junction_nanosplicer/main.nf @@ -15,13 +15,13 @@ process junctions_nanosplicer{ tuple val(barcode), path(csv) output: - path("${barcode}/JWR_check_parsed.csv") + path("${barcode}/${barcode}_JWR_check_parsed.csv") tuple val(barcode), path("${barcode}/${barcode}_identified_SPvariants.csv"), emit: identified_SPvariants script: """ mkdir ${barcode} cd ${barcode}/ - Rscript /Junctions_NanoSplicer.R -c ../${txt} -j ../${csv} + Rscript /Junctions_NanoSplicer.R -c ../${txt} -j ../${csv} -b ${barcode} """ } \ No newline at end of file diff --git a/src/nf_modules/rna_count/main.nf b/src/nf_modules/rna_count/main.nf index 06afba66cb2379922e83b700cbe43fab3b21b9c8..448f5986a596a4a5f0d8a6a0d80ff538daf208d0 100644 --- a/src/nf_modules/rna_count/main.nf +++ b/src/nf_modules/rna_count/main.nf @@ -23,6 +23,6 @@ process rna_count{ """ mkdir ${barcode} cd ${barcode}/ - Rscript /HBV_RNAs_count.R -s ../${spvariants} -c ../${classification} + Rscript /HBV_RNAs_count.R -s ../${spvariants} -c ../${classification} -b ${barcode} """ } diff --git a/src/nf_modules/start_positions/main.nf b/src/nf_modules/start_positions/main.nf index 668d50a5726464764d78c9d73061b12452631263..49958700302c91e5ff61d3a91d4b4edbd01cb1b1 100644 --- a/src/nf_modules/start_positions/main.nf +++ b/src/nf_modules/start_positions/main.nf @@ -22,7 +22,7 @@ process start_position_individuals{ """ mkdir ${barcode} cd ${barcode}/ - Rscript /Start_positions.R -i ../${start_position_counts} + Rscript /Start_positions.R -i ../${start_position_counts} -b ${barcode} mv Rplots.pdf ${barcode}_Rplots.pdf """ } \ No newline at end of file