From 36fe9db983b256cd2b0b55eaa4c1670e231cee07 Mon Sep 17 00:00:00 2001
From: xgrand <xavier.grand@ens-lyon.fr>
Date: Wed, 11 Sep 2024 14:14:32 +0200
Subject: [PATCH] README.md update

---
 README.md | 9 ++++++++-
 1 file changed, 8 insertions(+), 1 deletion(-)

diff --git a/README.md b/README.md
index 1450578..5fb398d 100755
--- a/README.md
+++ b/README.md
@@ -38,6 +38,10 @@ The arguments of this pipeline are described in the table below:
 | --chunks_size [int] | Chunck size, default = 1900 (refer to guppy manual). |
 | --help --h | Display this help message. |
 
+## Reference sequence
+
+The HBV reference sequence, genotype D ayw, is available in "data" folder.
+
 ## Contributing
 
 If you want to add more tools to this project, please read the [CONTRIBUTING.md](CONTRIBUTING.md).
@@ -49,7 +53,10 @@ If you want to add more tools to this project, please read the [CONTRIBUTING.md]
 
 ## License
 
-This project is licensed under the CeCiLL License- see the [LICENSE](LICENSE) file for details
+This project is licensed under the CeCiLL License- see the [LICENSE](LICENSE) file for details.
+
+The optional basecalling and demultiplexing steps may be carried out if necessary but are not executed automatically. 
+To execute these steps, it is essential to adhere to the guidelines provided with the Guppy software from Oxford Nanopore Technologies.
 
 ## To Do:
 
-- 
GitLab