version = "1.2.2" container_url = "lbmc/bowtie:${version}" params.index_fasta = "" params.index_fasta_out = "" process index_fasta { container = "${container_url}" label "big_mem_multi_cpus" tag "$file_id" if (params.index_fasta_out != "") { publishDir "results/${params.index_fasta_out}", mode: 'copy' } input: tuple val(file_id), path(fasta) output: tuple val(file_id), path("*.index*"), emit: index tuple val(file_id), path("*_report.txt"), emit: report script: """ bowtie-build --threads ${task.cpus} \ ${params.index_fasta} \ -f ${fasta} ${fasta.baseName}.index &> \ ${fasta.baseName}_bowtie_index_report.txt if grep -q "Error" ${fasta.baseName}_bowtie_index_report.txt; then exit 1 fi """ } params.mapping_fastq = "--very-sensitive" params.mapping_fastq_out = "" process mapping_fastq { container = "${container_url}" label "big_mem_multi_cpus" tag "$pair_id" if (params.mapping_fastq_out != "") { publishDir "results/${params.mapping_fastq_out}", mode: 'copy' } input: tuple val(index_id), path(index) tuple val(file_id), path(reads) output: tuple val(file_id), path("*.bam"), emit: bam path "*_report.txt", emit: report script: if (file_id instanceof List){ file_prefix = file_id[0] } else { file_prefix = file_id } index_id = index[0] for (index_file in index) { if (index_file =~ /.*\.1\.bt2/ && !(index_file =~ /.*\.rev\.1\.bt2/)) { index_id = ( index_file =~ /(.*)\.1\.bt2/)[0][1] } } if (reads.size() == 2) """ # -v specify the max number of missmatch, -k the number of match reported per # reads bowtie --best -v 3 -k 1 --sam -p ${task.cpus} ${index_id} \ ${params.mapping_fastq} \ -1 ${reads[0]} -2 ${reads[1]} 2> \ ${file_id}_bowtie_report_tmp.txt | \ samtools view -Sb - > ${file_id}.bam if grep -q "Error" ${file_id}_bowtie_report_tmp.txt; then exit 1 fi tail -n 19 ${file_id}_bowtie_report_tmp.txt > \ ${file_id}_bowtie_mapping_report.txt """ else """ bowtie --best -v 3 -k 1 --sam -p ${task.cpus} ${index_id} \ ${params.mapping_fastq} -q ${reads} 2> \ ${file_id}_bowtie_report_tmp.txt | \ samtools view -Sb - > ${file_id}.bam if grep -q "Error" ${file_id}_bowtie_report_tmp.txt; then exit 1 fi tail -n 19 ${file_id}_bowtie_report_tmp.txt > \ ${file_id}_bowtie_mapping_report.txt """ } params.mapping_fastq_pairedend = "" process mapping_fastq_pairedend { container = "${container_url}" label "big_mem_multi_cpus" tag "$pair_id" input: path index tuple val(pair_id), path(reads) output: tuple val(pair_id), path("*.bam"), emit: bam path "*_report.txt", emit: report script: index_id = index[0] for (index_file in index) { if (index_file =~ /.*\.1\.bt2/ && !(index_file =~ /.*\.rev\.1\.bt2/)) { index_id = ( index_file =~ /(.*)\.1\.bt2/)[0][1] } } """ # -v specify the max number of missmatch, -k the number of match reported per # reads bowtie --best -v 3 -k 1 --sam -p ${task.cpus} ${index_id} \ ${params.mapping_fastq_pairedend} \ -1 ${reads[0]} -2 ${reads[1]} 2> \ ${pair_id}_bowtie_report_tmp.txt | \ samtools view -Sb - > ${pair_id}.bam if grep -q "Error" ${pair_id}_bowtie_report_tmp.txt; then exit 1 fi tail -n 19 ${pair_id}_bowtie_report_tmp.txt > \ ${pair_id}_bowtie_mapping_report.txt """ } params.mapping_fastq_singleend = "" process mapping_fastq_singleend { container = "${container_url}" label "big_mem_multi_cpus" tag "$file_id" input: path index tuple val(file_id), path(reads) output: set file_id, "*.bam", emit: bam file "*_report.txt", emit: report script: index_id = index[0] for (index_file in index) { if (index_file =~ /.*\.1\.bt2/ && !(index_file =~ /.*\.rev\.1\.bt2/)) { index_id = ( index_file =~ /(.*)\.1\.bt2/)[0][1] } } """ bowtie --best -v 3 -k 1 --sam -p ${task.cpus} ${index_id} \ ${params.mapping_fastq_singleend} \ -q ${reads} 2> \ ${file_id}_bowtie_report_tmp.txt | \ samtools view -Sb - > ${file_id}.bam if grep -q "Error" ${file_id}_bowtie_report_tmp.txt; then exit 1 fi tail -n 19 ${file_id}_bowtie_report_tmp.txt > \ ${file_id}_bowtie_mapping_report.txt """ }