diff --git a/src/RNAseq.nf b/src/RNAseq.nf index a6792735b0617bc4353ffbbae9c4b71b28ea2a87..dd31c13f23ee844059f75ecb96a38a8b59338993 100644 --- a/src/RNAseq.nf +++ b/src/RNAseq.nf @@ -76,3 +76,60 @@ bedtools getfasta -name \ """ } +params.fasta = "$baseDir/data/bam/*.fasta" + +log.info "fasta files : ${params.fasta}" + +Channel + .fromPath( params.fasta ) + .ifEmpty { error "Cannot find any fasta files matching: ${params.fasta}" } + .set { fasta_file } + +process index_fasta { + tag "$fasta.baseName" + publishDir "results/mapping/index/", mode: 'copy' + + input: + file fasta from fasta_file + + output: + file "*.index*" into index_files + + script: +""" +kallisto index -k 31 --make-unique -i ${fasta.baseName}.index ${fasta} \ +> ${fasta.baseName}_kallisto_report.txt +""" +} + +params.index = "$baseDir/data/index/*.index.*" + +log.info "index files : ${params.index}" + +Channel + .fromPath( params.index ) + .ifEmpty { error "Cannot find any index files matching: ${params.index}" } + .set { index_files } + +process mapping_fastq { + tag "$reads" + cpus 4 + publishDir "results/mapping/quantification/", mode: 'copy' + + input: + file reads from fastq_files + file index from index_files.toList() + + output: + file "*" into counts_files + + script: +""" +mkdir ${reads[0].baseName} +kallisto quant -i ${index} -t ${task.cpus} \ +--bias --bootstrap-samples 100 -o ${reads[0].baseName} \ +${reads[0]} ${reads[1]} &> ${reads[0].baseName}_kallisto_report.txt +""" +} + +