diff --git a/src/nf_modules/gatk4/main.nf b/src/nf_modules/gatk4/main.nf index f0b13262b71a4e6e9f5076ead438f6fab256f803..c4f84bc8a0b1dd8cfef81ce7ff03f2c148b213dc 100644 --- a/src/nf_modules/gatk4/main.nf +++ b/src/nf_modules/gatk4/main.nf @@ -13,8 +13,9 @@ process variant_calling { tuple val(file_id), path("*.vcf"), emit: vcf script: + xmx_memory = task.memory - ~/\s*GB/ """ -gatk --java-options "-Xmx${task.memory}" -T HaplotypeCaller \ +gatk --java-options "-Xmx${xmx_memory}" -T HaplotypeCaller \ -nct ${task.cpus} \ -R ${fasta} \ -I ${bam} \ @@ -33,8 +34,9 @@ process filter_snp { output: tuple val(file_id), path("*_snp.vcf"), emit: vcf script: + xmx_memory = task.memory - ~/\s*GB/ """ -gatk --java-options "-Xmx${task.memory}" -T SelectVariants \ +gatk --java-options "-Xmx${xmx_memory}" -T SelectVariants \ -nct ${task.cpus} \ -R ${fasta} \ -V ${vcf} \ @@ -54,8 +56,9 @@ process filter_indels { output: tuple val(file_id), path("*_indel.vcf"), emit: vcf script: + xmx_memory = task.memory - ~/\s*GB/ """ -gatk --java-options "-Xmx${task.memory}" -T SelectVariants \ +gatk --java-options "-Xmx${xmx_memory}"-T SelectVariants \ -nct ${task.cpus} \ -R ${fasta} \ -V ${vcf} \ @@ -77,8 +80,9 @@ process high_confidence_snp { output: tuple val(file_id), path("*_snp.vcf"), emit: vcf script: + xmx_memory = task.memory - ~/\s*GB/ """ -gatk --java-options "-Xmx${task.memory}" -T VariantFiltration \ +gatk --java-options "-Xmx${xmx_memory}"-T VariantFiltration \ -nct ${task.cpus} \ -R ${fasta} \ -V ${vcf} \ @@ -101,8 +105,9 @@ process high_confidence_indels { output: tuple val(file_id), path("*_indel.vcf"), emit: vcf script: + xmx_memory = task.memory - ~/\s*GB/ """ -gatk --java-options "-Xmx${task.memory}" -T VariantFiltration \ +gatk --java-options "-Xmx${xmx_memory}" -T VariantFiltration \ -nct ${task.cpus} \ -R ${fasta} \ -V ${vcf} \ @@ -123,8 +128,9 @@ process recalibrate_snp_table { output: tuple val(file_id), path("recal_data_table"), emit: recal_table script: + xmx_memory = task.memory - ~/\s*GB/ """ -gatk --java-options "-Xmx${task.memory}" -T BaseRecalibrator \ +gatk --java-options "-Xmx${xmx_memory}" -T BaseRecalibrator \ -nct ${task.cpus} \ -R ${fasta} \ -I ${bam} \ @@ -146,8 +152,9 @@ process recalibrate_snp { output: tuple val(file_id), path("*.bam"), emit: bam script: + xmx_memory = task.memory - ~/\s*GB/ """ -gatk --java-options "-Xmx${task.memory}" -T PrintReads \ +gatk --java-options "-Xmx${xmx_memory}" -T PrintReads \ --use_jdk_deflater \ --use_jdk_inflater \ -nct ${task.cpus} \ @@ -169,8 +176,9 @@ process haplotype_caller { output: tuple val(file_id), path("*.gvcf"), emit: gvcf script: + xmx_memory = task.memory - ~/\s*GB/ """ -gatk --java-options "-Xmx${task.memory}" -T HaplotypeCaller \ +gatk --java-options "-Xmx${xmx_memory}" -T HaplotypeCaller \ -nct ${task.cpus} \ -R ${fasta} \ -I ${bam} \ @@ -191,8 +199,9 @@ process gvcf_genotyping { output: tuple val(file_id), path("*.vcf"), emit: vcf script: + xmx_memory = task.memory - ~/\s*GB/ """ -gatk --java-options "-Xmx${task.memory}" -T GenotypeGVCFs \ +gatk --java-options "-Xmx${xmx_memory}" -T GenotypeGVCFs \ -nct ${task.cpus} \ -R ${fasta} \ -V ${gvcf} \