diff --git a/src/nf_modules/ucsc/main.nf b/src/nf_modules/ucsc/main.nf index c63de6e227707552f5d51c3b0fc4cda34d9b8186..b4fe98dfac4378c00c828ee4119955d4e03fca83 100644 --- a/src/nf_modules/ucsc/main.nf +++ b/src/nf_modules/ucsc/main.nf @@ -18,7 +18,8 @@ process bedgraph_to_bigwig { # transform bed file of start-stop chromosome size to stop chromosome size awk -v OFS="\\t" '{print \$1, \$3}' ${bed} > chromsize.txt -bedGraphToBigWig ${bg} \ +sort -T ./ -k1,1 -k2,2n ${bg} > \ + bedGraphToBigWig - \ chromsize.txt \ ${bg.simpleName}_norm.bw """