diff --git a/src/example_marseq.nf b/src/example_marseq.nf index 8b8068a94774807cf39595721cb8c7b309773f91..fb3f8e8f41eb8828504fddcfc1f92bf2cd1f7f17 100644 --- a/src/example_marseq.nf +++ b/src/example_marseq.nf @@ -4,6 +4,7 @@ nextflow.enable.dsl=2 Testing pipeline for marseq scRNASeq analysis */ +include { adaptor_removal} from "./nf_modules/cutadapt/main.nf" include { index_fasta; count } from "./nf_modules/kb/main.nf" addParams( kb_protocol: "marsseq", count_out: "quantification/" @@ -57,6 +58,7 @@ channel .set { config_files } workflow { + adaptor_removal(fastq_files) index_fasta(fasta_files, cdna_files, gtf_files) - count(index_fasta.out.index, fastq_files, index_fasta.out.t2g, whitelist_files, config_files) + count(index_fasta.out.index, adaptor_removal.out.fastq, index_fasta.out.t2g, whitelist_files, config_files) }