diff --git a/src/nf_modules/gatk4/main.nf b/src/nf_modules/gatk4/main.nf index f2206d4c60c22700de8a1e093fc1ddb2a53296f0..51eea9dee6fd4c52a2bf0d53b4788346978493bc 100644 --- a/src/nf_modules/gatk4/main.nf +++ b/src/nf_modules/gatk4/main.nf @@ -15,11 +15,11 @@ process variant_calling { script: xmx_memory = "${task.memory}" - ~/\s*GB/ """ -gatk --java-options "-Xmx${xmx_memory}G" HaplotypeCallerSpark \ +gatk --java-Options "-Xmx${xmx_memory}G" HaplotypeCallerSpark \ --spark-master local[${task.cpus}] \ -R ${fasta} \ -I ${bam} \ - -o ${file_id}.vcf + -O ${file_id}.vcf """ } @@ -36,11 +36,11 @@ process filter_snp { script: xmx_memory = "${task.memory}" - ~/\s*GB/ """ -gatk --java-options "-Xmx${xmx_memory}G" SelectVariants \ +gatk --java-Options "-Xmx${xmx_memory}G" SelectVariants \ -R ${fasta} \ -V ${vcf} \ -selectType SNP \ - -o ${file_id}_snp.vcf + -O ${file_id}_snp.vcf """ } @@ -57,11 +57,11 @@ process filter_indels { script: xmx_memory = "${task.memory}" - ~/\s*GB/ """ -gatk --java-options "-Xmx${xmx_memory}G"-T SelectVariants \ +gatk --java-Options "-Xmx${xmx_memory}G"-T SelectVariants \ -R ${fasta} \ -V ${vcf} \ -selectType INDEL \ - -o ${file_id}_indel.vcf + -O ${file_id}_indel.vcf """ } @@ -80,12 +80,12 @@ process high_confidence_snp { script: xmx_memory = "${task.memory}" - ~/\s*GB/ """ -gatk --java-options "-Xmx${xmx_memory}G"-T VariantFiltration \ +gatk --java-Options "-Xmx${xmx_memory}G"-T VariantFiltration \ -R ${fasta} \ -V ${vcf} \ --filterExpression "${high_confidence_snp_filter}" \ --filterName "basic_snp_filter" \ - -o ${file_id}_filtered_snp.vcf + -O ${file_id}_filtered_snp.vcf """ } @@ -104,12 +104,12 @@ process high_confidence_indels { script: xmx_memory = "${task.memory}" - ~/\s*GB/ """ -gatk --java-options "-Xmx${xmx_memory}G" VariantFiltration \ +gatk --java-Options "-Xmx${xmx_memory}G" VariantFiltration \ -R ${fasta} \ -V ${vcf} \ --filterExpression "${high_confidence_indel_filter}" \ --filterName "basic_indel_filter" \ - -o ${file_id}_filtered_indel.vcf + -O ${file_id}_filtered_indel.vcf """ } @@ -126,13 +126,13 @@ process recalibrate_snp_table { script: xmx_memory = "${task.memory}" - ~/\s*GB/ """ -gatk --java-options "-Xmx${xmx_memory}G" BaseRecalibratorSpark \ +gatk --java-Options "-Xmx${xmx_memory}G" BaseRecalibratorSpark \ --spark-master local[${task.cpus}] \ -R ${fasta} \ -I ${bam} \ -knownSites ${snp_file} \ -knownSites ${indel_file} \ - -o recal_data_table + -O recal_data_table """ } @@ -150,13 +150,13 @@ process recalibrate_snp { script: xmx_memory = "${task.memory}" - ~/\s*GB/ """ -gatk --java-options "-Xmx${xmx_memory}G" PrintReads \ +gatk --java-Options "-Xmx${xmx_memory}G" PrintReads \ --use_jdk_deflater \ --use_jdk_inflater \ -R ${fasta} \ -I ${bam} \ -BQSR recal_data_table \ - -o ${file_id}_recal.bam + -O ${file_id}_recal.bam """ } @@ -173,13 +173,13 @@ process haplotype_caller { script: xmx_memory = "${task.memory}" - ~/\s*GB/ """ -gatk --java-options "-Xmx${xmx_memory}G" HaplotypeCallerSpark \ +gatk --java-Options "-Xmx${xmx_memory}G" HaplotypeCallerSpark \ --spark-master local[${task.cpus}] \ -R ${fasta} \ -I ${bam} \ -ERC GVCF \ -variant_index_type LINEAR -variant_index_parameter 128000 \ - -o ${file_id}.gvcf + -O ${file_id}.gvcf """ } @@ -196,10 +196,10 @@ process gvcf_genotyping { script: xmx_memory = "${task.memory}" - ~/\s*GB/ """ -gatk --java-options "-Xmx${xmx_memory}G" GenotypeGVCFs \ +gatk --java-Options "-Xmx${xmx_memory}G" GenotypeGVCFs \ -R ${fasta} \ -V ${gvcf} \ - -o ${file_id}_joint.vcf + -O ${file_id}_joint.vcf """ } @@ -216,12 +216,12 @@ process select_variants_snp { script: xmx_memory = "${task.memory}" - ~/\s*GB/ """ -gatk --java-options "-Xmx${xmx_memory}GG" SelectVariants \ +gatk --java-Options "-Xmx${xmx_memory}GG" SelectVariants \ --spark-master local[${task.cpus}] \ -R ${fasta} \ -V ${vcf} \ -selectType SNP \ - -o ${file_id}_joint_snp.vcf + -O ${file_id}_joint_snp.vcf """ } @@ -238,12 +238,12 @@ process select_variants_indels { script: xmx_memory = "${task.memory}" - ~/\s*GB/ """ -gatk --java-options "-Xmx${xmx_memory}G" SelectVariants \ +gatk --java-Options "-Xmx${xmx_memory}G" SelectVariants \ --spark-master local[${task.cpus}] \ -R ${fasta} \ -V ${vcf} \ -selectType INDEL \ - -o ${file_id}_joint_indel.vcf + -O ${file_id}_joint_indel.vcf """ } @@ -261,10 +261,10 @@ process personalized_genome { script: xmx_memory = "${task.memory}" - ~/\s*GB/ """ -gatk --java-options "-Xmx${xmx_memory}G" FastaAlternateReferenceMaker\ +gatk --java-Options "-Xmx${xmx_memory}G" FastaAlternateReferenceMaker\ -R ${reference} \ -V ${vcf} \ - -o ${file_id[0]}_genome.fasta + -O ${file_id[0]}_genome.fasta """ }