diff --git a/src/Rnaseq.nf b/src/Rnaseq.nf index 1709ae04d1608d472c864338f16b13bf2095a22c..0b9ec81e0d4130ac85215724e221f6a214f06c89 100644 --- a/src/Rnaseq.nf +++ b/src/Rnaseq.nf @@ -1,7 +1,21 @@ params.fastq = "$baseDir/data/fastq/*_{1,2}.fastq" +params.fasta = "$baseDir/data/fasta/*.fasta" +params.bed = "$baseDir/data/annot/*.bed" +log.info "fasta file : ${params.fasta}" +log.info "bed file : ${params.bed}" log.info "fastq files : ${params.fastq}" +Channel + .fromPath( params.fasta ) + .ifEmpty { error "Cannot find any fasta files matching: ${params.fasta}" } + .set { fasta_files } + +Channel + .fromPath( params.bed ) + .ifEmpty { error "Cannot find any bed files matching: ${params.bed}" } + .set { bed_files } + Channel .fromFilePairs( params.fastq ) .ifEmpty { error "Cannot find any fastq files matching: ${params.fastq}" } @@ -45,3 +59,24 @@ UrQt --t 20 --m ${task.cpus} --gz \ """ } +process fasta_from_bed { + tag "${bed.baseName}" + cpus 4 + publishDir "results/fasta/", mode: 'copy' + + input: + file fasta from fasta_files + file bed from bed_files + + output: + file "*_extracted.fasta" into fasta_files_extracted + + script: +""" +bedtools getfasta -name \ +-fi ${fasta} -bed ${bed} -fo ${bed.baseName}_extracted.fasta +""" +} + + +