diff --git a/Dockerfile b/Dockerfile
index ffef33215219ba08994b9ce185f1c47329cb2f81..2801363a58d02fc39b43ef56b902f5321be4d9b9 100644
--- a/Dockerfile
+++ b/Dockerfile
@@ -1,4 +1,4 @@
-FROM rocker/tidyverse:4.4.1
+FROM rocker/tidyverse:4.4.3
 USER root
 WORKDIR /root/app-recipe
 
@@ -16,9 +16,9 @@ RUN if [ -f "apt.txt" ]; then \
 
 # Install R packages
 USER root
-COPY install.R .
-RUN if [ -f "install.R" ]; then \
-  Rscript install.R; \
+COPY install_rnaseq.R .
+RUN if [ -f "install_rnaseq.R" ]; then \
+  Rscript install_rnaseq.R; \
   fi
 
 # Install tools from source
@@ -84,15 +84,15 @@ RUN bash /tmp/Miniforge3.sh -b -p /home/rstudio/conda && \
 
 ### Install conda env for user 'rstudio'
 
-COPY environment_uengs.yml /opt/environment_uengs.yml
+COPY environment_rnaseq.yml /opt/environment_rnaseq.yml
 ##
 RUN source /home/rstudio/conda/etc/profile.d/conda.sh &&\
-    conda env create -f /opt/environment_uengs.yml && \
+    conda env create -f /opt/environment_rnaseq.yml && \
     conda clean -a -y
 
 
-COPY bashrc_ext.txt /tmp/bashrc_ext.txt
-RUN cat  /tmp/bashrc_ext.txt >> /home/rstudio/.bashrc
+COPY bashrc_ext_rnaseq.txt /tmp/bashrc_ext_rnaseq.txt
+RUN cat  /tmp/bashrc_ext_rnaseq.txt >> /home/rstudio/.bashrc
 
 ##
 ##
diff --git a/bashrc_ext_rnaseq.txt b/bashrc_ext_rnaseq.txt
new file mode 100644
index 0000000000000000000000000000000000000000..9256da4f5bb8c8c1514c573ab8fdefbfb43c54cd
--- /dev/null
+++ b/bashrc_ext_rnaseq.txt
@@ -0,0 +1,3 @@
+
+#activate conda env
+conda activate rnaseq
\ No newline at end of file
diff --git a/bashrc_ext.txt b/bashrc_ext_uengs.txt
similarity index 100%
rename from bashrc_ext.txt
rename to bashrc_ext_uengs.txt
diff --git a/build_docker.sh b/build_docker.sh
index 9b7f85930bd0b3ec730a4ebc2655f3f7ca8fd682..8305faa566b883fde993c977d943f24341f5e196 100644
--- a/build_docker.sh
+++ b/build_docker.sh
@@ -2,7 +2,7 @@
 set -euo pipefail +o nounset
 
 IMAGE_NAME="tp_ngs"
-TAG="v2024.2"
+TAG="v2025.1"
 REPO=carinerey/$IMAGE_NAME:$TAG
 
 DOCKERFILE_DIR="."
diff --git a/environment_rnaseq.yml b/environment_rnaseq.yml
index b2932f135de6c2d90caed15e2c274408704b425e..6d049c0487a6abe02cc99d1dd97f682fcecff778 100644
--- a/environment_rnaseq.yml
+++ b/environment_rnaseq.yml
@@ -4,13 +4,13 @@ channels:
   - bioconda
 dependencies:
   - seqtk
-  - fastp=0.23.4
+  - fastp=0.24.0
   - salmon=1.10.3
   - star=2.7.11b
   - htseq=2.0.5
   - blast=2.16.0
   - fastqc=0.12.1
-  - multiqc=1.24
-  - samtools=1.20
+  - multiqc=1.27.1
+  - samtools=1.21
   - bamtools=2.5.2
   - bedtools=2.31.1
diff --git a/install_rnaseq.R b/install_rnaseq.R
new file mode 100644
index 0000000000000000000000000000000000000000..9c09ab7dabc92fff382586a15ad05808657fbf35
--- /dev/null
+++ b/install_rnaseq.R
@@ -0,0 +1,19 @@
+options(Ncpus = parallel::detectCores())
+options(repos = c(CRAN = "https://cran.rstudio.com"))
+
+# Installation of standard R packages
+install.packages(c("remotes", "devtools"))
+
+# Installation of packages with BiocManager
+install.packages("BiocManager")
+BiocManager::install(
+    c("biomaRt",
+      "clusterProfiler",
+      "DESeq2",
+      "EnhancedVolcano",
+      "ggpubr",
+      "apeglm",
+      "scater",
+      "rhdf5",
+      "idr"
+    ), ask = FALSE)
diff --git a/install.R b/install_uengs.R
similarity index 100%
rename from install.R
rename to install_uengs.R