diff --git a/src/nf_modules/MUSIC/peak_calling_paired.config b/src/nf_modules/MUSIC/peak_calling_paired.config deleted file mode 100644 index 4685752be3d8e66da4e76aeffc82ce58d50389b3..0000000000000000000000000000000000000000 --- a/src/nf_modules/MUSIC/peak_calling_paired.config +++ /dev/null @@ -1,30 +0,0 @@ -profiles { - docker { - docker.temp = 'auto' - docker.enabled = true - process { - $compute_mappability { - container = "music:6613c53" - } - $music_preprocessing { - container = "music:6613c53" - } - $music_computation { - container = "music:6613c53" - } - } - } - sge { - process{ - $compute_mappability { - beforeScript = "module purge; module load MUSIC/6613c53" - } - $music_preprocessing { - beforeScript = "module purge; module load MUSIC/6613c53" - } - $music_computation { - beforeScript = "module purge; module load MUSIC/6613c53" - } - } - } -} diff --git a/src/nf_modules/MUSIC/tests.sh b/src/nf_modules/MUSIC/tests.sh new file mode 100755 index 0000000000000000000000000000000000000000..7c6ad078ad2c27cbb378f8cdd6c505be9527fdef --- /dev/null +++ b/src/nf_modules/MUSIC/tests.sh @@ -0,0 +1,8 @@ +cp results/training/bams/sBNLN18.bam results/training/bams/sBNLN18_control.bam +./nextflow src/nf_modules/MUSIC/peak_calling_single.nf \ + -c src/nf_modules/MUSIC/peak_calling_single.config \ + -profile docker \ + --fasta "results/training/fasta/*.fasta" \ + --bam "results/training/bams/s*.bam" \ + --index "results/training/mapping/index/*" \ + --read_size 50 --frag_size 300 diff --git a/src/training_dataset.nf b/src/training_dataset.nf index 7796067a208e8c6a2da13ef49467844da377466e..c6f48e882a82eaa7437a409c7500c7045224893e 100644 --- a/src/training_dataset.nf +++ b/src/training_dataset.nf @@ -147,7 +147,7 @@ if ( params.fastq_paired != "" ) { set file_id, "*.fastq" into fastq_files_extracted script: """ - samtools fastq -1 s${file_id}_R1.fastq -2 s${file_id}_R2.fastq -f 0x2 ${bam} + samtools fastq -1 s${file_id}_R1.fastq -2 s${file_id}_R2.fastq -F 0x4 ${bam} """ } @@ -163,7 +163,7 @@ if ( params.fastq_paired != "" ) { set file_id, "*.bam" into filtered_bam_files_paired script: """ - samtools view -@ ${task.cpus} -hb ${bam} -f 0x2 > f${file_id}.bam + samtools view -@ ${task.cpus} -hb ${bam} -F 0x4 > f${file_id}.bam """ }