diff --git a/src/nf_modules/Bowtie2/indexing.config b/src/nf_modules/Bowtie2/indexing.config index 434889bc922c66338e8459cb6990fb43e0d04ea1..c53d855a63f599cad802c3f12e0114c875bd3ee5 100644 --- a/src/nf_modules/Bowtie2/indexing.config +++ b/src/nf_modules/Bowtie2/indexing.config @@ -3,21 +3,33 @@ profiles { docker.temp = 'auto' docker.enabled = true process { - withName: mapping_fastq { + withName: index_fasta { container = "bowtie2:2.3.4.1" + cpus = 4 + } + } + } + singularity { + singularity.enabled = true + process { + withName: index_fasta { + container = "file://bin/bowtie2:2.3.4.1.sif" + cpus = 4 } } } psmn { process{ - withName: mapping_fastq { + withName: index_fasta { beforeScript = "source /usr/share/lmod/lmod/init/bash; module use ~/privatemodules" module = "Bowtie2/2.3.4.1" executor = "sge" clusterOptions = "-m e -cwd -V" + cpus = 16 memory = "20GB" time = "12h" - queue = 'monointeldeb128' + queue = 'E5-2670deb128A,E5-2670deb128B,E5-2670deb128C,E5-2670deb128D,E5-2670deb128E,E5-2670deb128F' + penv = 'openmp16' } } } diff --git a/src/nf_modules/Bowtie2/indexing.nf b/src/nf_modules/Bowtie2/indexing.nf index 4f3cde5e990f3528f2f000c8e68131edafdecf97..45318049b076f6dcad4d7ef2335185796a5e7234 100644 --- a/src/nf_modules/Bowtie2/indexing.nf +++ b/src/nf_modules/Bowtie2/indexing.nf @@ -9,7 +9,6 @@ Channel process index_fasta { tag "$fasta.baseName" - cpus 4 publishDir "results/mapping/index/", mode: 'copy' input: diff --git a/src/nf_modules/Bowtie2/mapping_paired.config b/src/nf_modules/Bowtie2/mapping_paired.config index 30150ff97c6cbc333746b0a86d08c8da8db34c13..27933c3bf5582ae32fc7012598e8f05bb642a09b 100644 --- a/src/nf_modules/Bowtie2/mapping_paired.config +++ b/src/nf_modules/Bowtie2/mapping_paired.config @@ -5,6 +5,16 @@ profiles { process { withName: mapping_fastq { container = "bowtie2:2.3.4.1" + cpus = 4 + } + } + } + singularity { + singularity.enabled = true + process { + withName: mapping_fastq { + container = "file://bin/bowtie2:2.3.4.1.sif" + cpus = 4 } } } @@ -15,6 +25,7 @@ profiles { module = "Bowtie2/2.3.4.1:SAMtools/1.7" executor = "sge" clusterOptions = "-m e -cwd -V" + cpus = 16 memory = "30GB" time = "24h" queue = 'E5-2670deb128A,E5-2670deb128B,E5-2670deb128C,E5-2670deb128D,E5-2670deb128E,E5-2670deb128F' diff --git a/src/nf_modules/Bowtie2/mapping_paired.nf b/src/nf_modules/Bowtie2/mapping_paired.nf index 7422b143606e720138d07ea3b04fe16d4301c117..dc4667400ec33eed593b1b93decf42994d8777ad 100644 --- a/src/nf_modules/Bowtie2/mapping_paired.nf +++ b/src/nf_modules/Bowtie2/mapping_paired.nf @@ -15,7 +15,6 @@ Channel process mapping_fastq { tag "$pair_id" - cpus 4 publishDir "results/mapping/bams/", mode: 'copy' input: diff --git a/src/nf_modules/Bowtie2/mapping_single.config b/src/nf_modules/Bowtie2/mapping_single.config index 30150ff97c6cbc333746b0a86d08c8da8db34c13..27933c3bf5582ae32fc7012598e8f05bb642a09b 100644 --- a/src/nf_modules/Bowtie2/mapping_single.config +++ b/src/nf_modules/Bowtie2/mapping_single.config @@ -5,6 +5,16 @@ profiles { process { withName: mapping_fastq { container = "bowtie2:2.3.4.1" + cpus = 4 + } + } + } + singularity { + singularity.enabled = true + process { + withName: mapping_fastq { + container = "file://bin/bowtie2:2.3.4.1.sif" + cpus = 4 } } } @@ -15,6 +25,7 @@ profiles { module = "Bowtie2/2.3.4.1:SAMtools/1.7" executor = "sge" clusterOptions = "-m e -cwd -V" + cpus = 16 memory = "30GB" time = "24h" queue = 'E5-2670deb128A,E5-2670deb128B,E5-2670deb128C,E5-2670deb128D,E5-2670deb128E,E5-2670deb128F' diff --git a/src/nf_modules/Bowtie2/mapping_single.nf b/src/nf_modules/Bowtie2/mapping_single.nf index 66676991aae710a5263d763dee2ad7dbde6b7a51..1b7954ec174cccd0ad70ee02b79b3fd5b1a91589 100644 --- a/src/nf_modules/Bowtie2/mapping_single.nf +++ b/src/nf_modules/Bowtie2/mapping_single.nf @@ -15,7 +15,6 @@ Channel process mapping_fastq { tag "$file_id" - cpus 4 publishDir "results/mapping/bams/", mode: 'copy' input: diff --git a/src/nf_modules/Bowtie2/tests.sh b/src/nf_modules/Bowtie2/tests.sh index ab1483824cd1f9755d09495cd050c45cc4a82d30..01e349a7ccb3cce9c6afc9244e90abd9bb200656 100755 --- a/src/nf_modules/Bowtie2/tests.sh +++ b/src/nf_modules/Bowtie2/tests.sh @@ -1,17 +1,41 @@ ./nextflow src/nf_modules/Bowtie2/indexing.nf \ -c src/nf_modules/Bowtie2/indexing.config \ -profile docker \ - --fasta "data/tiny_dataset/fasta/tiny_v2.fasta" + --fasta "data/tiny_dataset/fasta/tiny_v2.fasta" \ + -resume ./nextflow src/nf_modules/Bowtie2/mapping_single.nf \ -c src/nf_modules/Bowtie2/mapping_single.config \ -profile docker \ --index "data/tiny_dataset/fasta/*.bt2" \ - --fastq "data/tiny_dataset/fastq/tiny*_S.fastq" + --fastq "data/tiny_dataset/fastq/tiny*_S.fastq" \ + -resume ./nextflow src/nf_modules/Bowtie2/mapping_paired.nf \ -c src/nf_modules/Bowtie2/mapping_paired.config \ -profile docker \ --index "data/tiny_dataset/fasta/*.bt2" \ - --fastq "data/tiny_dataset/fastq/tiny*_R{1,2}.fastq" + --fastq "data/tiny_dataset/fastq/tiny*_R{1,2}.fastq" \ + -resume +if [ -x "$(command -v singularity)" ]; then +./nextflow src/nf_modules/Bowtie2/indexing.nf \ + -c src/nf_modules/Bowtie2/indexing.config \ + -profile singularity \ + --fasta "data/tiny_dataset/fasta/tiny_v2.fasta" \ + -resume + +./nextflow src/nf_modules/Bowtie2/mapping_single.nf \ + -c src/nf_modules/Bowtie2/mapping_single.config \ + -profile singularity \ + --index "data/tiny_dataset/fasta/*.bt2" \ + --fastq "data/tiny_dataset/fastq/tiny*_S.fastq" \ + -resume + +./nextflow src/nf_modules/Bowtie2/mapping_paired.nf \ + -c src/nf_modules/Bowtie2/mapping_paired.config \ + -profile singularity \ + --index "data/tiny_dataset/fasta/*.bt2" \ + --fastq "data/tiny_dataset/fastq/tiny*_R{1,2}.fastq" \ + -resume +fi diff --git a/src/singularity_modules/Bowtie2/2.3.4.1/Bowtie2.def b/src/singularity_modules/Bowtie2/2.3.4.1/Bowtie2.def new file mode 100644 index 0000000000000000000000000000000000000000..73a177e449b3e3acb2330909edfb055cac9cb709 --- /dev/null +++ b/src/singularity_modules/Bowtie2/2.3.4.1/Bowtie2.def @@ -0,0 +1,23 @@ +Bootstrap: docker +From: ubuntu:18.04 + +%labels +MAINTAINER Laurent Modolo + +%post +BOWTIE2_VERSION=2.3.4.1 +SAMTOOLS_VERSION=1.7 +PACKAGES="bowtie2=${BOWTIE2_VERSION}* \ +samtools=${SAMTOOLS_VERSION}* \ +perl=5.26.1*" + +apt-get update && \ +apt-get install -y --no-install-recommends ${PACKAGES} && \ +apt-get clean + +%environment +export BOWTIE2_VERSION=2.3.4.1 +export SAMTOOLS_VERSION=1.7 + +%runscript +exec /bin/bash "$@" diff --git a/src/singularity_modules/Bowtie2/2.3.4.1/build.sh b/src/singularity_modules/Bowtie2/2.3.4.1/build.sh new file mode 100755 index 0000000000000000000000000000000000000000..398fb1c79a1bb8b34d7dd1327824021906357abe --- /dev/null +++ b/src/singularity_modules/Bowtie2/2.3.4.1/build.sh @@ -0,0 +1,3 @@ +#/bin/sh +sudo singularity build --force bin/bowtie2:2.3.4.1.sif src/singularity_modules/Bowtie2/2.3.4.1/Bowtie2.def +singularity sign bin/bowtie2:2.3.4.1.sif