diff --git a/src/RNAseq.nf b/src/RNAseq.nf index 37f5bdedf653963727d7d487e59b5163371ea65e..a6792735b0617bc4353ffbbae9c4b71b28ea2a87 100644 --- a/src/RNAseq.nf +++ b/src/RNAseq.nf @@ -1,11 +1,23 @@ params.fastq = "$baseDir/data/fastq/*_{1,2}.fastq" /* we can use now a param -fastq to specify where are fastq files. this path is the default path */ +params.fasta = "$baseDir/data/fasta/*.fasta" +params.bed = "$baseDir/data/annot/*.bed" log.info "fastq files : ${params.fastq}" +log.info "fasta file : ${params.fasta}" +log.info "bed file : ${params.bed}" Channel .fromFilePairs( params.fastq ) .ifEmpty { error "Cannot find any fastq files matching: ${params.fastq}" } .set { fastq_files } +Channel + .fromPath( params.fasta ) + .ifEmpty { error "Cannot find any fasta files matching: ${params.fasta}" } + .set { fasta_files } +Channel + .fromPath( params.bed ) + .ifEmpty { error "Cannot find any bed files matching: ${params.bed}" } + .set { bed_files } process adaptor_removal { tag "$pair_id" @@ -45,4 +57,22 @@ UrQt --t 20 --m ${task.cpus} --gz \ """ } +process fasta_from_bed { + tag "${bed.baseName}" + cpus 4 + publishDir "results/fasta/", mode: 'copy' + + input: + file fasta from fasta_files + file bed from bed_files + + output: + file "*_extracted.fasta" into fasta_files_extracted + + script: +""" +bedtools getfasta -name \ +-fi ${fasta} -bed ${bed} -fo ${bed.baseName}_extracted.fasta +""" +}