params.fastq = "$baseDir/data/fastq/*_{1,2}.fastq" params.index = "$baseDir/data/index/*.index.*" log.info "fastq files : ${params.fastq}" log.info "index files : ${params.index}" Channel .fromFilePairs( params.fastq ) .ifEmpty { error "Cannot find any fastq files matching: ${params.fastq}" } .set { fastq_files } Channel .fromPath( params.index ) .ifEmpty { error "Cannot find any index files matching: ${params.index}" } .set { index_files } process mapping_fastq { tag "$pair_id" cpus 4 publishDir "results/mapping/quantification/", mode: 'copy' input: set pair_id, file(reads) from fastq_files file index from index_files.toList() output: file "*" into counts_files script: index_name = (index[0].baseName =~ /(.*)\.\d/)[0][1] """ rsem-calculate-expression --bowtie2 \ --bowtie2-path \$(which bowtie2 | sed 's/bowtie2\$//g') \ --bowtie2-sensitivity-level "very_sensitive" \ -output-genome-bam -p ${task.cpus} \ --paired-end ${reads[0]} ${reads[1]} ${index_name} ${pair_id} \ > ${pair_id}_rsem_bowtie2_report.txt """ }