diff --git a/src/RNAseq.nf b/src/RNAseq.nf index 0bfc24211b9c144f691e9d9b058171bcb6bdabb5..4b89ec31213a4a9fb0350d56136cfe695833bbea 100644 --- a/src/RNAseq.nf +++ b/src/RNAseq.nf @@ -44,4 +44,38 @@ UrQt --t 20 --m ${task.cpus} --gz \ --out ${reads[0].baseName}_trim_R1.fastq.gz --outpair ${reads[1].baseName}_trim_R2.fastq.gz \ > ${reads[0].baseName}_trimming_report.txt """ -} \ No newline at end of file +} + +params.fastq = "$baseDir/data/fasta/*.fasta" +params.bed = "$baseDir/data/annot/*.bed" + +log.info "fasta file : ${params.fasta}" +log.info "bed file : ${params.bed}" + +Channel + .fromPath( params.fasta ) + .ifEmpty { error "Cannot find any fasta files matching: ${params.fasta}" } + .set { fasta_files } +Channel + .fromPath( params.bed ) + .ifEmpty { error "Cannot find any bed files matching: ${params.bed}" } + .set { bed_files } + +process fasta_from_bed { + tag "${bed.baseName}" + cpus 4 + publishDir "results/fasta/", mode: 'copy' + + input: + file fasta from fasta_files + file bed from bed_files + + output: + file "*_extracted.fasta" into fasta_files_extracted + + script: +""" +bedtools getfasta -name \ +-fi ${fasta} -bed ${bed} -fo ${bed.baseName}_extracted.fasta +""" +}