diff --git a/README.md b/README.md index 680e2caccf30e47133368550b3d2effa13583f9b..a89c6449f623945e4b19fde51751c25dfd8dde4a 100644 --- a/README.md +++ b/README.md @@ -37,16 +37,20 @@ where counts K ij for gene i, sample j are modeled using a Negative Binomial dis fitted mean µ ij and a gene-specific dispersion parameter α i . The fitted mean is composed of a sample-specific size factor s j and a parameter q ij proportional to the expected true concentration of fragments for sample j. The coefficients β i give the log2 fold changes for gene i for each column of the model matrix X. <br/> -### 4) Wald Test H0: +### 4) Wald Test: -Test if Log(FC) = 0 <br/> +H0: Test if Log(FC) = 0 <br/> With DESeq2, the Wald test is the default used for hypothesis testing when comparing two groups. The Wald test is a test of hypothesis usually performed on parameters that have been estimated by maximum likelihood. The Wald test is also a standard way to extract a P value from a regression fit. ## Investigations -Let µ and alpha constant between samples, replicates and genes + +N.B: Don't forget to change your home path in ```src/main.R``` + + +Let µ and alpha constant between samples, replicates and genes (absolutely false IRL) 1) µ effect