From 5af4d96e3bffc06ab3f59aa0a808ac0ec408298b Mon Sep 17 00:00:00 2001 From: Arnaud Duvermy <arnaud.duvermy@ens-lyon.fr> Date: Thu, 10 Feb 2022 13:48:39 +0100 Subject: [PATCH] Update README.md --- README.md | 11 ++++++----- 1 file changed, 6 insertions(+), 5 deletions(-) diff --git a/README.md b/README.md index aeb7303..d72f0e4 100644 --- a/README.md +++ b/README.md @@ -28,18 +28,19 @@ Fit a curve to wise gene dispersion estimate ### 3) Fit linear model -The differential expression analysis uses a generalized linear model of the form: +The differential expression analysis uses a generalized linear model of the form: <br/> Kij ∼ NB(µij , α i )<br/> µij = s j q ij <br/> log 2 (q ij ) = x j. β i <br/> where counts K ij for gene i, sample j are modeled using a Negative Binomial distribution with fitted mean µ ij and a gene-specific dispersion parameter α i . The fitted mean is composed of a -sample-specific size factor s j and a parameter q ij proportional to the expected true concentration -of fragments for sample j. The coefficients β i give the log2 fold changes for gene i for each col- -umn of the model matrix X. The sample-specific size factors can be see as the logFC between condition. +sample-specific size factor s j and a parameter q ij proportional to the expected true concentration of fragments for sample j. The coefficients β i give the log2 fold changes for gene i for each column of the model matrix X. +x j. β i can be see as the logFC between condition (for treated condition). -4) Wald Test H0: Log(FC) = 0 +### 4) Wald Test H0: + +Test if Log(FC) = 0 -- GitLab