diff --git a/src/RNAseq_XGR.nf b/src/RNAseq_XGR.nf index a5f8ff5c84327c71eadcdb232491d97ed288cbec..b336891e50860c01f9758d3cd88d6d162c5b9e4e 100644 --- a/src/RNAseq_XGR.nf +++ b/src/RNAseq_XGR.nf @@ -64,7 +64,7 @@ params.fastq = "${project}/fastq/*_{1,2}.fq.gz" params.gtf = "" params.fasta = "" params.idx = "" -params.filter_bam = "-F 268 -f 1 -q 10" +params.filter_bam_mapped = "-F 268 -f 1 -q 10" params.fastp_out = "$params.project/fastp/" params.star_mapping_fastq_out = "$params.project/STAR/" @@ -109,7 +109,8 @@ include { index_with_gtf } from "./nf_modules/star/main_2.7.8a.nf" include { mapping_fastq } from "./nf_modules/star/main_2.7.8a.nf" include { mapping_withindex } from "./nf_modules/star/main_2.7.8a.nf" include { htseq_count } from "./nf_modules/htseq/main.nf" -include { filter_bam } from "./nf_modules/samtools/main.nf" + +include { filter_bam_mapped } from "./nf_modules/samtools/main.nf" include { stats_bam } from "./nf_modules/samtools/main.nf" include { sort_bam } from "./nf_modules/samtools/main.nf" include { index_bam } from "./nf_modules/samtools/main.nf" @@ -137,7 +138,7 @@ workflow { index_with_gtf(genome_file, gtf_file.collect()) mapping_fastq(index_with_gtf.out.index.collect(), fastp.out.fastq) - + filter_bam_mapped(mapping_fastq.out.bam) } else { idx_genome = "${params.idx}" @@ -146,10 +147,13 @@ workflow { .set { genome_indexed_input } genome_indexed_input.view() mapping_withindex(genome_indexed_input.collect(), fastp.out.fastq) - htseq_count(mapping_withindex.out.bam, gtf_file) + stats_bam(mapping_withindex.out.bam) + filter_bam_mapped(mapping_withindex.out.bam) } - htseq_count(mapping_fastq.out.bam, gtf_file) + sort_bam(filter_bam_mapped.out.bam) + index_bam(sort_bam_mapped.out.bam) + htseq_count(sort_bam_mapped.out.bam, gtf_file) //########################## QUALITY CHECKS ###################