diff --git a/src/RNAseq_XGR.nf b/src/RNAseq_XGR.nf index f456e7095c3a810f8f4559a6ee15f4292bccd246..bd67d8bf4ef9dc48a04e661fa61b7938fc050276 100644 --- a/src/RNAseq_XGR.nf +++ b/src/RNAseq_XGR.nf @@ -116,7 +116,7 @@ workflow { //########################## PREPROCESSING #################### // fastp - fastp(fastq_files) + fastp(fastq_files.collect()) //########################## QUALITY CHECKS ################### @@ -142,7 +142,7 @@ workflow { .set { genome_file } index_with_gtf(genome_file, gtf_file.collect()) - mapping_fastq(index_with_gtf.out.index, fastq_files) + mapping_fastq(index_with_gtf.out.index, fastp.out.fastq) } else { idx_genome = "${params.idx}/*" @@ -150,7 +150,7 @@ workflow { .fromPath( idx_genome ) .ifEmpty { error "Cannot find idexed genome reference files" } .set { genome_indexed_input } - mapping_fastq(genome_indexed_input, fastq_files) + mapping_fastq(genome_indexed_input, fastp.out.fastq) } //######################## HTseq COUNT #########################