diff --git a/src/commands.sh b/src/commands.sh index 07be5a8a4d848652bc0425cd83c1edc3e284f087..b01d5acbd04103d3c24bf681680705e4197fcc39 100644 --- a/src/commands.sh +++ b/src/commands.sh @@ -358,7 +358,7 @@ done ############################################################ -python3 -m src.gc_content -B results/bed_file/Far_CTCF_2000_both_ddx_down_with0_exon.bed results/bed_file/CTCF_2000_both_ddx_down_with0_exon.bed -b ddx_down ddx_down_ctcf -g data/Homo_sapiens.GRCh37.dna.primary_assembly.fa -f "exons" -e 1000 +python3 -m src.gc_content -B results/bed_file/Far_CTCF_2000_both_ddx_down_with0_exon.bed results/bed_file/CTCF_2000_both_ddx_down_with0_exon.bed -b ddx_down ddx_down_ctcf -g data/Homo_sapiens.GRCh37.dna.primary_assembly.fa -f "exons" -e 2000 @@ -480,6 +480,7 @@ for i in ${!list_names[*]}; do --show_replicate n \ --figure_type metagene \ --nb_bin 100 \ + -y 0.15 0.4 \ --norm "results/figures/coef_table/tmp_cov_table_design_exp_all_replicates_${nbed}_100bin_10000_nt-around-25-bin_bin0_norm.txt" mv results/figures/metagene_${cname}_100bin_10000_nt-around-25-bin_file_norm.pdf results/figures/all_replicates_metagene_${cname}_100bin_10000_nt-around-25-bin_file_norm.pdf @@ -690,6 +691,14 @@ done # SHY5Y # ############################## +# Creating a bed file only containing expressed gene in 5y cells + +python3 -m src.bed_handler.filter_bed \ + -b data/bed/gene.bed \ + -f data/5y_expressed_genes_basemean\>5.txt \ + -c id \ + -o 5y_expressed_gene.bed + # Create a bed file containing bins=('None' 0 99) bin_names=('' '_b0_norm' '_b99_norm') @@ -697,8 +706,8 @@ for i in ${!bins[@]}; do python3 -m src.visu \ --design data/bigwig_SHY5Y/design_exp_all_replicates.txt \ --bw_folder data/bigwig_SHY5Y/ \ - --region_bed data/bed/gene.bed \ - --region_name all_gene \ + --region_bed results/bed_file/5y_expressed_gene.bed \ + --region_name all_expressed_gene \ --output results/figures/ \ --border_name TSS TTS \ --environment 10000 25 \ @@ -707,11 +716,11 @@ for i in ${!bins[@]}; do --nb_bin 100 \ --norm ${bins[$i]} - mv results/figures/metagene_all_gene_100bin_10000_nt-around-25-bin${bin_names[$i]}.pdf results/figures/metagene_5y_rnaseq_all_gene${bin_names[$i]}.pdf + mv results/figures/metagene_all_expressed_gene_100bin_10000_nt-around-25-bin${bin_names[$i]}.pdf results/figures/metagene_5y_rnaseq_all_expressed_gene${bin_names[$i]}.pdf done tts_sizes=(10000 50000) -bins=(25 125) +bins=(25 25) for i in ${!tts_sizes[@]}; do size=${tts_sizes[$i]} bin=${bins[$i]} @@ -720,26 +729,26 @@ for i in ${!tts_sizes[@]}; do # Create a bed file containing all gene containing one DDX-down exons python3 -m src.bed_handler.bed_resize \ - -b data/bed/gene.bed \ + -b results/bed_file/5y_expressed_gene.bed \ -s ${size} \ -r "end" \ -t "outer" \ - -o all_gene_end${size}.bed + -o all_expressed_gene_end${size}.bed python3 -m src.visu \ --design data/bigwig_SHY5Y/design_exp_all_replicates.txt \ --bw_folder data/bigwig_SHY5Y/ \ - --region_bed results/bed_file/all_gene_end${size}.bed \ - --region_name all_gene \ + --region_bed results/bed_file/all_expressed_gene_end${size}.bed \ + --region_name all_expressed_gene \ --output results/figures/ \ --border_name TTS '' \ --environment 0 0 \ --show_replicate n \ --figure_type metagene \ --nb_bin ${bin} \ - --norm "results/figures/coef_table/tmp_cov_table_design_exp_all_replicates_gene_100bin_10000_nt-around-25-bin_bin99_norm.txt" + --norm "results/figures/coef_table/tmp_cov_table_design_exp_all_replicates_5y_expressed_gene_100bin_10000_nt-around-25-bin_bin99_norm.txt" - mv results/figures/metagene_all_gene_${bin}bin_0_nt-around-0-bin_file_norm.pdf results/figures/metagene_5y_rnaseq_TTS2${kb_size}kb_all_gene_${bin}bin_0_nt-around-0-bin_TTS_norm.pdf + mv results/figures/metagene_all_expressed_gene_${bin}bin_0_nt-around-0-bin_file_norm.pdf results/figures/metagene_5y_rnaseq_TTS2${kb_size}kb_expressed_gene_${bin}bin_bin_TTS_norm.pdf done