diff --git a/src/commands.sh b/src/commands.sh index e38aac4003937e614c21f4ee8561129cc7ea9d63..3462df0b726926627fce01d40d4574a0ec998dd1 100644 --- a/src/commands.sh +++ b/src/commands.sh @@ -779,6 +779,7 @@ mv results/figures/metagene_all_gene_100bin_0_nt-around-0-bin.pdf results/figure ##################################################### # Metagene figure of last exons in readthrough genes near (<=2000 nt) or far (> 2000nt) from a CTCF file and last exons from non readthrough genes +# figures 5C ##################################################### # Bed file containing the last exons of expressed gene with readthrough @@ -834,4 +835,51 @@ for i in ${!list_names[*]}; do mv results/figures/metagene_${cname}_30bin_10000_nt-around-25-bin_file_norm.pdf results/figures/all_replicates_metaexon_${cname}_30bin_10000_nt-around-25-bin_file_norm.pdf done -python3 -m src.gc_content -B results/bed_file/readthrough_last_exon_near_CTCF_2000_both_ddx_with0_exon.bed results/bed_file/readthrough_last_exon_far_CTCF_2000_both_ddx_with0_exon.bed results/bed_file/no_readthrough_expressed_last_exon.bed -b readthrough_ctcf readthrough no_readthrough -g data/Homo_sapiens.GRCh37.dna.primary_assembly.fa -f "exons" -e 2000 \ No newline at end of file + +########################################################### +# Figures siPP vs siCTRL pour ddx_down_ctcf, +# , ddx_down figure 5B +########################################################### + + +list_names=(ddx_down_ctcf ddx_down) +bed_names=(CTCF_2000_both_ddx_down_with0_exon.bed Far_CTCF_2000_both_ddx_down_with0_exon.bed) +for i in ${!list_names[*]}; do + cname=${list_names[i]} + bed=${bed_names[i]} + gbed=${bed/exon\.bed/gene-dup.bed} + nbed=${gbed/\.bed/} + python3 -m src.visu \ + --design data/design_exp_all_replicates.txt \ + --bw_folder data/bigwig/ \ + --region_bed results/bed_file/${gbed} \ + --region_name ${cname} \ + --output results/figures/ \ + --border_name TSS TTS \ + --environment 10000 25 \ + --show_replicate n \ + --figure_type metagene \ + --nb_bin 100 \ + --norm '0' + + rm results/figures/metagene_${cname}_100bin_10000_nt-around-25-bin_b0_norm.pdf + + python3 -m src.visu \ + --design data/design_exp_all_replicates.txt \ + --bw_folder data/bigwig/ \ + --region_bed results/bed_file/${bed} \ + --region_name ${cname} \ + --output results/figures/ \ + --border_name " " " " \ + --environment 10000 25 \ + --show_replicate n \ + --figure_type metagene \ + --nb_bin 30 \ + -y 0.15 0.4 \ + --norm "results/figures/coef_table/tmp_cov_table_design_exp_all_replicates_${nbed}_100bin_10000_nt-around-25-bin_bin0_norm.txt" + + mv results/figures/metagene_${cname}_30bin_10000_nt-around-25-bin_file_norm.pdf results/figures/all_replicates_metagene_${cname}_100bin_10000_nt-around-25-bin_file_norm.pdf +done + +python3 -m src.gc_content -B results/bed_file/readthrough_last_exon_near_CTCF_2000_both_ddx_with0_exon.bed results/bed_file/readthrough_last_exon_far_CTCF_2000_both_ddx_with0_exon.bed results/bed_file/no_readthrough_expressed_last_exon.bed -b readthrough_ctcf readthrough no_readthrough -g data/Homo_sapiens.GRCh37.dna.primary_assembly.fa -f "exons" -e 2000 +