params.fastq = "$baseDir/data/fastq/*.fastq" params.index = "$baseDir/data/index/*.index*" params.mean = 200 params.sd = 100 log.info "fastq files : ${params.fastq}" log.info "index files : ${params.index}" log.info "mean read size: ${params.mean}" log.info "sd read size: ${params.sd}" Channel .fromPath( params.fastq ) .ifEmpty { error "Cannot find any fastq files matching: ${params.fastq}" } .set { fastq_files } Channel .fromPath( params.index ) .ifEmpty { error "Cannot find any index files matching: ${params.index}" } .set { index_files } process mapping_fastq { tag "$reads.baseName" cpus 4 publishDir "results/mapping/quantification/", mode: 'copy' input: file reads from fastq_files file index from index_files.toList() output: file "*" into count_files script: """ mkdir ${reads.baseName} kallisto quant -i ${index} -t ${task.cpus} --single \ --bias --bootstrap-samples 100 -o ${reads.baseName} \ -l ${params.mean} -s ${params.sd} \ ${reads} > ${reads.baseName}_kallisto_report.txt """ }