params.bam = "$baseDir/data/bam/*.bam" log.info "bams files : ${params.bam}" Channel .fromPath( params.bam ) .ifEmpty { error "Cannot find any bam files matching: ${params.bam}" } .set { bam_files } process split_bam { tag "$bam.baseName" cpus 2 input: file bam from bam_files output: file "*_forward.bam*" into forward_bam_files file "*_reverse.bam*" into reverse_bam_files script: """ samtools view -hb -F 0x10 ${bam} > ${bam}_forward.bam & samtools view -hb -f 0x10 ${bam} > ${bam}_reverse.bam """ }