params.fastq = "$baseDir/data/fastq/*.fastq" params.index = "$baseDir/data/index/*.index*" params.mean = 200 params.sd = 100 log.info "fastq files : ${params.fastq}" log.info "index files : ${params.index}" log.info "mean read size: ${params.mean}" log.info "sd read size: ${params.sd}" Channel .fromPath( params.fastq ) .ifEmpty { error "Cannot find any fastq files matching: ${params.fastq}" } .map { it -> [(it.baseName =~ /([^\.]*)/)[0][1], it]} .set { fastq_files } Channel .fromPath( params.index ) .ifEmpty { error "Cannot find any index files matching: ${params.index}" } .set { index_files } process mapping_fastq { tag "$file_id" publishDir "results/mapping/quantification/", mode: 'copy' input: set file_id, file(reads) from fastq_files file index from index_files.collect() output: file "*" into count_files script: index_id = index[0] for (index_file in index) { if (index_file =~ /.*\.1\.bt2/ && !(index_file =~ /.*\.rev\.1\.bt2/)) { index_id = ( index_file =~ /(.*)\.1\.bt2/)[0][1] } } """ rsem-calculate-expression --bowtie2 \ --bowtie2-path \$(which bowtie2 | sed 's/bowtie2\$//g') \ --bowtie2-sensitivity-level "very_sensitive" \ --fragment-length-mean ${params.mean} --fragment-length-sd ${params.sd} \ --output-genome-bam -p ${task.cpus} \ ${reads} ${index_id} ${file_id} \ 2> ${file_id}_rsem_bowtie2_report.txt if grep -q "Error" ${file_id}_rsem_bowtie2_report.txt; then exit 1 fi """ }