diff --git a/src/nf_modules/BWA/indexing.config b/src/nf_modules/BWA/indexing.config index 2936204f3dc8475073f98ae24c4dcdb70a6dfda9..367e0dfdc90a4b3a32241fed08459b1cfc3a9fa4 100644 --- a/src/nf_modules/BWA/indexing.config +++ b/src/nf_modules/BWA/indexing.config @@ -5,6 +5,16 @@ profiles { process { withName: index_fasta { container = "bwa:0.7.17" + cpus = 4 + } + } + } + singularity { + singularity.enabled = true + process { + withName: index_fasta { + container = "file://bin/bwa:0.7.17.sif" + cpus = 4 } } } @@ -15,9 +25,11 @@ profiles { module = "BWA/0.7.17" executor = "sge" clusterOptions = "-m e -cwd -V" + cpus = 16 memory = "30GB" - time = "12h" - queue = 'monointeldeb128' + time = "24h" + queue = 'E5-2670deb128A,E5-2670deb128B,E5-2670deb128C,E5-2670deb128D,E5-2670deb128E,E5-2670deb128F' + penv = 'openmp16' } } } diff --git a/src/nf_modules/BWA/indexing.nf b/src/nf_modules/BWA/indexing.nf index 67ea3287bf374808ab4b01007fe05c381d711151..09096eeaa0b7e9b77d7078c4edd68734a7d68dbf 100644 --- a/src/nf_modules/BWA/indexing.nf +++ b/src/nf_modules/BWA/indexing.nf @@ -10,7 +10,6 @@ Channel process index_fasta { tag "$fasta_id" - cpus 4 publishDir "results/mapping/index/", mode: 'copy' input: diff --git a/src/nf_modules/BWA/mapping_paired.config b/src/nf_modules/BWA/mapping_paired.config index ce8f7f4582171b245b9a1162779007916f999122..5535fd104b1b62d933c6dcfed34e2d6364fd5503 100644 --- a/src/nf_modules/BWA/mapping_paired.config +++ b/src/nf_modules/BWA/mapping_paired.config @@ -5,6 +5,16 @@ profiles { process { withName: mapping_fastq { container = "bwa:0.7.17" + cpus = 4 + } + } + } + singularity { + singularity.enabled = true + process { + withName: mapping_fastq { + container = "file://bin/bwa:0.7.17.sif" + cpus = 4 } } } @@ -15,6 +25,7 @@ profiles { module = "BWA/0.7.17" executor = "sge" clusterOptions = "-m e -cwd -V" + cpus = 16 memory = "30GB" time = "24h" queue = 'E5-2670deb128A,E5-2670deb128B,E5-2670deb128C,E5-2670deb128D,E5-2670deb128E,E5-2670deb128F' diff --git a/src/nf_modules/BWA/mapping_paired.nf b/src/nf_modules/BWA/mapping_paired.nf index 5ac50ef555dc42b2415f76e72c1a36e932637894..7309c6052fb2e670d29ef51b7b547c59ce092c4a 100644 --- a/src/nf_modules/BWA/mapping_paired.nf +++ b/src/nf_modules/BWA/mapping_paired.nf @@ -17,7 +17,6 @@ Channel process mapping_fastq { tag "$reads" - cpus 4 publishDir "results/mapping/sam/", mode: 'copy' input: diff --git a/src/nf_modules/BWA/tests.sh b/src/nf_modules/BWA/tests.sh index 1f3150cf8debc2d6be9ccf19167d25418af42223..8f3c52daf10f6c4c95da2d24e502b2118b17cd8b 100755 --- a/src/nf_modules/BWA/tests.sh +++ b/src/nf_modules/BWA/tests.sh @@ -1,7 +1,8 @@ ./nextflow src/nf_modules/BWA/indexing.nf \ -c src/nf_modules/BWA/indexing.config \ -profile docker \ - --fasta "data/tiny_dataset/fasta/tiny_v2.fasta" + --fasta "data/tiny_dataset/fasta/tiny_v2.fasta" \ + -resume # ./nextflow src/nf_modules/BWA/mapping_single.nf \ # -c src/nf_modules/BWA/mapping_single.config \ @@ -13,5 +14,29 @@ -c src/nf_modules/BWA/mapping_paired.config \ -profile docker \ --index "results/mapping/index/tiny_v2*" \ - --fastq "data/tiny_dataset/fastq/tiny*_R{1,2}.fastq" + --fastq "data/tiny_dataset/fastq/tiny*_R{1,2}.fastq" \ + -resume + +if [ -x "$(command -v singularity)" ]; then +./nextflow src/nf_modules/BWA/indexing.nf \ + -c src/nf_modules/BWA/indexing.config \ + -profile singularity \ + --fasta "data/tiny_dataset/fasta/tiny_v2.fasta" \ + -resume + + +# ./nextflow src/nf_modules/BWA/mapping_single.nf \ +# -c src/nf_modules/BWA/mapping_single.config \ +# -profile singularity \ +# --index "results/mapping/index/tiny_v2.index" \ +# --fastq "data/tiny_dataset/fastq/tiny*_S.fastq" + +./nextflow src/nf_modules/BWA/mapping_paired.nf \ + -c src/nf_modules/BWA/mapping_paired.config \ + -profile singularity \ + --index "results/mapping/index/tiny_v2*" \ + --fastq "data/tiny_dataset/fastq/tiny*_R{1,2}.fastq" \ + -resume + +fi diff --git a/src/singularity_modules/BWA/0.7.17/build.sh b/src/singularity_modules/BWA/0.7.17/build.sh new file mode 100755 index 0000000000000000000000000000000000000000..340a8c219741a03182c5832ec78f92f0da3b652a --- /dev/null +++ b/src/singularity_modules/BWA/0.7.17/build.sh @@ -0,0 +1,2 @@ +#/bin/sh +sudo singularity build --force bin/bwa:0.7.17.sif src/singularity_modules/BWA/0.7.17/BWA.def