diff --git a/src/nf_modules/gatk4/main.nf b/src/nf_modules/gatk4/main.nf index 51eea9dee6fd4c52a2bf0d53b4788346978493bc..a92b0ee3e9c74dea969e5571fbbb3ca0a1b8f73a 100644 --- a/src/nf_modules/gatk4/main.nf +++ b/src/nf_modules/gatk4/main.nf @@ -15,7 +15,7 @@ process variant_calling { script: xmx_memory = "${task.memory}" - ~/\s*GB/ """ -gatk --java-Options "-Xmx${xmx_memory}G" HaplotypeCallerSpark \ +gatk --java-options "-Xmx${xmx_memory}G" HaplotypeCallerSpark \ --spark-master local[${task.cpus}] \ -R ${fasta} \ -I ${bam} \ @@ -36,7 +36,7 @@ process filter_snp { script: xmx_memory = "${task.memory}" - ~/\s*GB/ """ -gatk --java-Options "-Xmx${xmx_memory}G" SelectVariants \ +gatk --java-options "-Xmx${xmx_memory}G" SelectVariants \ -R ${fasta} \ -V ${vcf} \ -selectType SNP \ @@ -57,7 +57,7 @@ process filter_indels { script: xmx_memory = "${task.memory}" - ~/\s*GB/ """ -gatk --java-Options "-Xmx${xmx_memory}G"-T SelectVariants \ +gatk --java-options "-Xmx${xmx_memory}G"-T SelectVariants \ -R ${fasta} \ -V ${vcf} \ -selectType INDEL \ @@ -80,7 +80,7 @@ process high_confidence_snp { script: xmx_memory = "${task.memory}" - ~/\s*GB/ """ -gatk --java-Options "-Xmx${xmx_memory}G"-T VariantFiltration \ +gatk --java-options "-Xmx${xmx_memory}G"-T VariantFiltration \ -R ${fasta} \ -V ${vcf} \ --filterExpression "${high_confidence_snp_filter}" \ @@ -104,7 +104,7 @@ process high_confidence_indels { script: xmx_memory = "${task.memory}" - ~/\s*GB/ """ -gatk --java-Options "-Xmx${xmx_memory}G" VariantFiltration \ +gatk --java-options "-Xmx${xmx_memory}G" VariantFiltration \ -R ${fasta} \ -V ${vcf} \ --filterExpression "${high_confidence_indel_filter}" \ @@ -126,7 +126,7 @@ process recalibrate_snp_table { script: xmx_memory = "${task.memory}" - ~/\s*GB/ """ -gatk --java-Options "-Xmx${xmx_memory}G" BaseRecalibratorSpark \ +gatk --java-options "-Xmx${xmx_memory}G" BaseRecalibratorSpark \ --spark-master local[${task.cpus}] \ -R ${fasta} \ -I ${bam} \ @@ -150,7 +150,7 @@ process recalibrate_snp { script: xmx_memory = "${task.memory}" - ~/\s*GB/ """ -gatk --java-Options "-Xmx${xmx_memory}G" PrintReads \ +gatk --java-options "-Xmx${xmx_memory}G" PrintReads \ --use_jdk_deflater \ --use_jdk_inflater \ -R ${fasta} \ @@ -173,7 +173,7 @@ process haplotype_caller { script: xmx_memory = "${task.memory}" - ~/\s*GB/ """ -gatk --java-Options "-Xmx${xmx_memory}G" HaplotypeCallerSpark \ +gatk --java-options "-Xmx${xmx_memory}G" HaplotypeCallerSpark \ --spark-master local[${task.cpus}] \ -R ${fasta} \ -I ${bam} \ @@ -196,7 +196,7 @@ process gvcf_genotyping { script: xmx_memory = "${task.memory}" - ~/\s*GB/ """ -gatk --java-Options "-Xmx${xmx_memory}G" GenotypeGVCFs \ +gatk --java-options "-Xmx${xmx_memory}G" GenotypeGVCFs \ -R ${fasta} \ -V ${gvcf} \ -O ${file_id}_joint.vcf @@ -216,7 +216,7 @@ process select_variants_snp { script: xmx_memory = "${task.memory}" - ~/\s*GB/ """ -gatk --java-Options "-Xmx${xmx_memory}GG" SelectVariants \ +gatk --java-options "-Xmx${xmx_memory}GG" SelectVariants \ --spark-master local[${task.cpus}] \ -R ${fasta} \ -V ${vcf} \ @@ -238,7 +238,7 @@ process select_variants_indels { script: xmx_memory = "${task.memory}" - ~/\s*GB/ """ -gatk --java-Options "-Xmx${xmx_memory}G" SelectVariants \ +gatk --java-options "-Xmx${xmx_memory}G" SelectVariants \ --spark-master local[${task.cpus}] \ -R ${fasta} \ -V ${vcf} \ @@ -261,7 +261,7 @@ process personalized_genome { script: xmx_memory = "${task.memory}" - ~/\s*GB/ """ -gatk --java-Options "-Xmx${xmx_memory}G" FastaAlternateReferenceMaker\ +gatk --java-options "-Xmx${xmx_memory}G" FastaAlternateReferenceMaker\ -R ${reference} \ -V ${vcf} \ -O ${file_id[0]}_genome.fasta