def summary_params = NfcoreSchema.paramsSummaryMap(workflow, params) // Validate input parameters WorkflowHic.initialise(params, log) // Check mandatory parameters if (params.input) { ch_input = file(params.input) } else { exit 1, 'Input samplesheet not specified!' } // Digestion parameters if (params.digestion){ restriction_site = params.digestion ? params.digest[ params.digestion ].restriction_site ?: false : false ch_restriction_site = Channel.value(restriction_site) //str seq avec ^ pour cut ligation_site = params.digestion ? params.digest[ params.digestion ].ligation_site ?: false : false ch_ligation_site = Channel.value(ligation_site) //str seq }else if (params.dnase){ ch_restriction_site = Channel.empty() ch_ligation_site = Channel.empty() }else{ exit 1, "Ligation motif not found. Please either use the `--digestion` parameters or specify the `--restriction_site` and `--ligation_site`. For DNase Hi-C, please use '--dnase' option" } Channel.fromPath( params.fasta ) .ifEmpty { exit 1, "Genome index: Fasta file not found: ${params.fasta}" } .map{it->[[:],it]} .set { ch_fasta } include { INPUT_CHECK } from '../subworkflows/local/input_check' include { PREPARE_GENOME } from '../subworkflows/local/prepare_genome' include { HICSTUFF_SUB } from '../subworkflows/local/hicstuff_sub' workflow HICSTUFF { INPUT_CHECK ( ch_input ) PREPARE_GENOME( ch_fasta, ch_restriction_site ) HICSTUFF_SUB( INPUT_CHECK.out.reads, PREPARE_GENOME.out.index, ch_ligation_site, params.digestion, //str enzyme ch_fasta, PREPARE_GENOME.out.chromosome_size ) }