diff --git a/bin/mapped_2hic_dnase.py b/bin/mapped_2hic_dnase.py index ff593666f03c5b82928ced06b941be296d6169ea..c417907a4483bd33bc6a6e0ceeb752d5c3d32121 100755 --- a/bin/mapped_2hic_dnase.py +++ b/bin/mapped_2hic_dnase.py @@ -362,7 +362,6 @@ if __name__ == "__main__": if cur_handler is not None: if not r1.is_unmapped and not r2.is_unmapped: - ##reorient reads to ease duplicates removal or1, or2 = get_ordered_reads(r1, r2) or1_chrom = samfile.get_reference_name(or1.reference_id) diff --git a/bin/mapped_2hic_fragments.py b/bin/mapped_2hic_fragments.py index cc0e40b472dfb764ba1bf2f550c00c79bce7bd3f..a1298716ce83c4e99931534357c03634fd5d8301 100755 --- a/bin/mapped_2hic_fragments.py +++ b/bin/mapped_2hic_fragments.py @@ -619,7 +619,6 @@ if __name__ == "__main__": r2_chrom = None if r1_resfrag is not None or r2_resfrag is not None: - interactionType = get_interaction_type(r1, r1_chrom, r1_resfrag, r2, r2_chrom, r2_resfrag, verbose) dist = get_PE_fragment_size(r1, r2, r1_resfrag, r2_resfrag, interactionType) cdist = get_cis_dist(r1, r2) diff --git a/bin/mergeSAM.py b/bin/mergeSAM.py index 82ab8c34d979240d12de57b1f2510a54a79d2ca9..d670ec2c6a94b89035ab417b4146806099c2c5aa 100755 --- a/bin/mergeSAM.py +++ b/bin/mergeSAM.py @@ -71,7 +71,6 @@ def get_read_name(read): def sam_flag(read1, read2, hr1, hr2): - f1 = read1.flag f2 = read2.flag diff --git a/modules.json b/modules.json index 45540ca9ea0c4c8493525e58d8671176087348dc..6d7a0306685215f83fe5c69f51f6f54c1927fa04 100644 --- a/modules.json +++ b/modules.json @@ -7,52 +7,52 @@ "nf-core": { "bowtie2/align": { "branch": "master", - "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c", + "git_sha": "603ecbd9f45300c9788f197d2a15a005685b4220", "installed_by": ["modules"] }, "bowtie2/build": { "branch": "master", - "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c", + "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", "installed_by": ["modules"] }, "cooler/balance": { "branch": "master", - "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c", + "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", "installed_by": ["modules"] }, "cooler/cload": { "branch": "master", - "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c", + "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", "installed_by": ["modules"] }, "cooler/dump": { "branch": "master", - "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c", + "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", "installed_by": ["modules"] }, "cooler/makebins": { "branch": "master", - "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c", + "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", "installed_by": ["modules"] }, "cooler/zoomify": { "branch": "master", - "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c", + "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", "installed_by": ["modules"] }, "custom/dumpsoftwareversions": { "branch": "master", - "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c", + "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", "installed_by": ["modules"] }, "custom/getchromsizes": { "branch": "master", - "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c", + "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", "installed_by": ["modules"] }, "fastqc": { "branch": "master", - "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c", + "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", "installed_by": ["modules"] } } diff --git a/modules/local/cooltools/eigscis.nf b/modules/local/cooltools/eigscis.nf index feaa30007099cae34a18eaa8105a3367e260e1b6..873bd243cd02102f327cba853197d39bc0ea860e 100644 --- a/modules/local/cooltools/eigscis.nf +++ b/modules/local/cooltools/eigscis.nf @@ -9,7 +9,7 @@ process COOLTOOLS_EIGSCIS { conda "bioconda::cooltools=0.5.1 bioconda::ucsc-bedgraphtobigwig=377" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/mulled-v2-c81d8d6b6acf4714ffaae1a274527a41958443f6:cc7ea58b8cefc76bed985dcfe261cb276ed9e0cf-0' : - 'quay.io/biocontainers/mulled-v2-c81d8d6b6acf4714ffaae1a274527a41958443f6:cc7ea58b8cefc76bed985dcfe261cb276ed9e0cf-0' }" + 'biocontainers/mulled-v2-c81d8d6b6acf4714ffaae1a274527a41958443f6:cc7ea58b8cefc76bed985dcfe261cb276ed9e0cf-0' }" input: tuple val(meta), path(cool), val(resolution) diff --git a/modules/local/cooltools/insulation.nf b/modules/local/cooltools/insulation.nf index 8a9127ea47824f9541c4faf2263d8e61030468c3..af53529ef80baa12dbd81bd0a2b8fa3ec2122cfa 100644 --- a/modules/local/cooltools/insulation.nf +++ b/modules/local/cooltools/insulation.nf @@ -9,7 +9,7 @@ process COOLTOOLS_INSULATION { conda "bioconda::cooltools=0.5.1" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/cooltools:0.5.1--py37h37892f8_0' : - 'quay.io/biocontainers/cooltools:0.5.1--py37h37892f8_0' }" + 'biocontainers/cooltools:0.5.1--py37h37892f8_0' }" input: tuple val(meta), path(cool) diff --git a/modules/local/hicexplorer/hicFindTADs.nf b/modules/local/hicexplorer/hicFindTADs.nf index b6cae335c22111407b3d782e95a067ed087645e0..d86dc837e29e56aa4ba88cd2c66962444046647b 100644 --- a/modules/local/hicexplorer/hicFindTADs.nf +++ b/modules/local/hicexplorer/hicFindTADs.nf @@ -8,7 +8,7 @@ process HIC_FIND_TADS { conda "bioconda::hicexplorer=3.7.2" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/hicexplorer:3.7.2--pyhdfd78af_1' : - 'quay.io/biocontainers/hicexplorer:3.7.2--pyhdfd78af_1' }" + 'biocontainers/hicexplorer:3.7.2--pyhdfd78af_1' }" input: tuple val(meta), path(cool) diff --git a/modules/local/hicexplorer/hicPlotDistVsCounts.nf b/modules/local/hicexplorer/hicPlotDistVsCounts.nf index 3e37d3fe7b466dbe45b2f19c220e2f9a65a07a9a..1143a05d3767ba4a9289e6f423cb7dc768d3d197 100644 --- a/modules/local/hicexplorer/hicPlotDistVsCounts.nf +++ b/modules/local/hicexplorer/hicPlotDistVsCounts.nf @@ -9,7 +9,7 @@ process HIC_PLOT_DIST_VS_COUNTS { conda "bioconda::hicexplorer=3.7.2" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/hicexplorer:3.7.2--pyhdfd78af_1' : - 'quay.io/biocontainers/hicexplorer:3.7.2--pyhdfd78af_1' }" + 'biocontainers/hicexplorer:3.7.2--pyhdfd78af_1' }" input: tuple val(meta), path(cool) diff --git a/modules/local/hicpro/bowtie2_merge.nf b/modules/local/hicpro/bowtie2_merge.nf index 7a4e96be9aaf2470a10ca19672591e0f84853602..64aa6e6c05fd9e729ac26a0704f33b802f7dbb0c 100644 --- a/modules/local/hicpro/bowtie2_merge.nf +++ b/modules/local/hicpro/bowtie2_merge.nf @@ -5,7 +5,7 @@ process MERGE_BOWTIE2{ conda "bioconda::samtools=1.15.1" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/samtools:1.15.1--h1170115_0' : - 'quay.io/biocontainers/samtools:1.15.1--h1170115_0' }" + 'biocontainers/samtools:1.15.1--h1170115_0' }" input: tuple val(meta), path(bam1), path(bam2) diff --git a/modules/local/hicpro/build_contact_maps.nf b/modules/local/hicpro/build_contact_maps.nf index fd633a984f26b4cccc901a92e983cf0acfaa8eed..34b27aab498c8e1c45355d01b991d5900a619f33 100644 --- a/modules/local/hicpro/build_contact_maps.nf +++ b/modules/local/hicpro/build_contact_maps.nf @@ -5,15 +5,15 @@ process BUILD_CONTACT_MAPS{ conda "conda-forge::sed=4.7" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/ubuntu:20.04' : - 'ubuntu:20.04' }" + 'quay.io/nf-core/ubuntu:20.04' }" input: - tuple val(meta), path(vpairs), val(resolution) - tuple val(meta2), path(chrsize) + tuple val(meta), path(vpairs), val(resolution) + tuple val(meta2), path(chrsize) output: tuple val(meta), val(resolution), path("*.matrix"), path("*.bed"), emit: maps - + script: def prefix = task.ext.prefix ?: "${meta.id}" """ diff --git a/modules/local/hicpro/combine_mates.nf b/modules/local/hicpro/combine_mates.nf index da95be819a03560d75db2bc80ab3e8b72bf774aa..b0d828e3200bcb00fb4b94dcb071091ae352b7f7 100644 --- a/modules/local/hicpro/combine_mates.nf +++ b/modules/local/hicpro/combine_mates.nf @@ -5,7 +5,7 @@ process COMBINE_MATES { conda "conda-forge::python=3.9 bioconda::pysam=0.19.0" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/mulled-v2-c6ff206325681cbb9c9ef890bb8de554172c0483:713df51cd897ceb893b9a6e6420f527d83c2ed95-0' : - 'quay.io/biocontainers/mulled-v2-c6ff206325681cbb9c9ef890bb8de554172c0483:713df51cd897ceb893b9a6e6420f527d83c2ed95-0'}" + 'biocontainers/mulled-v2-c6ff206325681cbb9c9ef890bb8de554172c0483:713df51cd897ceb893b9a6e6420f527d83c2ed95-0'}" input: tuple val(meta), path(bam) diff --git a/modules/local/hicpro/dnase_mapping_stats.nf b/modules/local/hicpro/dnase_mapping_stats.nf index 64cb0792e4d93dfcc1b1e7d0b5d50bf7e0b75c73..8e85113944f79dc0b155ee9be441b2badf5c0245 100644 --- a/modules/local/hicpro/dnase_mapping_stats.nf +++ b/modules/local/hicpro/dnase_mapping_stats.nf @@ -5,7 +5,7 @@ process MAPPING_STATS_DNASE { conda "bioconda::samtools=1.15.1" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/samtools:1.15.1--h1170115_0' : - 'quay.io/biocontainers/samtools:1.15.1--h1170115_0' }" + 'biocontainers/samtools:1.15.1--h1170115_0' }" input: diff --git a/modules/local/hicpro/get_restriction_fragments.nf b/modules/local/hicpro/get_restriction_fragments.nf index affc2d986a339d239602c6e66ad6f0d50517505e..56cd74a24a7d5102aad25cbe7032e31e2c4a1452 100644 --- a/modules/local/hicpro/get_restriction_fragments.nf +++ b/modules/local/hicpro/get_restriction_fragments.nf @@ -5,7 +5,7 @@ process GET_RESTRICTION_FRAGMENTS { conda "conda-forge::python=3.9 conda-forge::numpy=1.22.3" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/mulled-v2-c6ff206325681cbb9c9ef890bb8de554172c0483:713df51cd897ceb893b9a6e6420f527d83c2ed95-0' : - 'quay.io/biocontainers/mulled-v2-c6ff206325681cbb9c9ef890bb8de554172c0483:713df51cd897ceb893b9a6e6420f527d83c2ed95-0'}" + 'biocontainers/mulled-v2-c6ff206325681cbb9c9ef890bb8de554172c0483:713df51cd897ceb893b9a6e6420f527d83c2ed95-0'}" input: tuple val(meta), path(fasta) diff --git a/modules/local/hicpro/get_valid_interaction.nf b/modules/local/hicpro/get_valid_interaction.nf index cc148371e52e04e96ca8dc9461a46ce80216b85e..bebd42197471c214ce0cde63c88ea89224387e76 100644 --- a/modules/local/hicpro/get_valid_interaction.nf +++ b/modules/local/hicpro/get_valid_interaction.nf @@ -5,7 +5,7 @@ process GET_VALID_INTERACTION { conda "conda-forge::python=3.9 bioconda::pysam=0.19.0 bioconda::bx-python=0.8.13" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/mulled-v2-c6ff206325681cbb9c9ef890bb8de554172c0483:713df51cd897ceb893b9a6e6420f527d83c2ed95-0' : - 'quay.io/biocontainers/mulled-v2-c6ff206325681cbb9c9ef890bb8de554172c0483:713df51cd897ceb893b9a6e6420f527d83c2ed95-0'}" + 'biocontainers/mulled-v2-c6ff206325681cbb9c9ef890bb8de554172c0483:713df51cd897ceb893b9a6e6420f527d83c2ed95-0'}" input: tuple val(meta), path(bam) diff --git a/modules/local/hicpro/get_valid_interaction_dnase.nf b/modules/local/hicpro/get_valid_interaction_dnase.nf index 142d0cbd4f042fb0106c7e0380515f7b54c79953..d62318f87feac914b8b07796792fa18e363eef7e 100644 --- a/modules/local/hicpro/get_valid_interaction_dnase.nf +++ b/modules/local/hicpro/get_valid_interaction_dnase.nf @@ -5,7 +5,7 @@ process GET_VALID_INTERACTION_DNASE { conda "conda-forge::python=3.9 bioconda::pysam=0.19.0 bioconda::bx-python=0.8.13" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/mulled-v2-c6ff206325681cbb9c9ef890bb8de554172c0483:713df51cd897ceb893b9a6e6420f527d83c2ed95-0' : - 'quay.io/biocontainers/mulled-v2-c6ff206325681cbb9c9ef890bb8de554172c0483:713df51cd897ceb893b9a6e6420f527d83c2ed95-0'}" + 'biocontainers/mulled-v2-c6ff206325681cbb9c9ef890bb8de554172c0483:713df51cd897ceb893b9a6e6420f527d83c2ed95-0'}" input: tuple val(meta), path(bam) diff --git a/modules/local/hicpro/hicpro2pairs.nf b/modules/local/hicpro/hicpro2pairs.nf index 2ba2a901b62791e733900b24da4f244cd2fbaba7..eb9b86b5e4ca0ace6549fe0c33a9dd1f63aaf5f3 100644 --- a/modules/local/hicpro/hicpro2pairs.nf +++ b/modules/local/hicpro/hicpro2pairs.nf @@ -5,7 +5,7 @@ process HICPRO2PAIRS { conda "bioconda::pairix=0.3.7" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/pairix:0.3.7--py36h30a8e3e_3' : - 'quay.io/biocontainers/pairix:0.3.7--py36h30a8e3e_3' }" + 'biocontainers/pairix:0.3.7--py36h30a8e3e_3' }" input: tuple val(meta), path(vpairs) diff --git a/modules/local/hicpro/merge_stats.nf b/modules/local/hicpro/merge_stats.nf index b25dc8a13d2f18bc5ae05742c5f15d77fd19627d..d9c2aecb5b2eb5c7574e139f7ffbbf3c8d3289de 100644 --- a/modules/local/hicpro/merge_stats.nf +++ b/modules/local/hicpro/merge_stats.nf @@ -5,7 +5,7 @@ process MERGE_STATS { conda "conda-forge::python=3.9" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/mulled-v2-c6ff206325681cbb9c9ef890bb8de554172c0483:713df51cd897ceb893b9a6e6420f527d83c2ed95-0' : - 'quay.io/biocontainers/mulled-v2-c6ff206325681cbb9c9ef890bb8de554172c0483:713df51cd897ceb893b9a6e6420f527d83c2ed95-0'}" + 'biocontainers/mulled-v2-c6ff206325681cbb9c9ef890bb8de554172c0483:713df51cd897ceb893b9a6e6420f527d83c2ed95-0'}" input: tuple val(meta), path(fstat) diff --git a/modules/local/hicpro/merge_valid_interaction.nf b/modules/local/hicpro/merge_valid_interaction.nf index 29e568a20997c0a313a87cf964f1f8c10327228b..25c437ef795882591b0f50fcf4a235f29dc60822 100644 --- a/modules/local/hicpro/merge_valid_interaction.nf +++ b/modules/local/hicpro/merge_valid_interaction.nf @@ -5,7 +5,7 @@ process MERGE_VALID_INTERACTION { conda "conda-forge::gawk=5.1.0" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/ubuntu:20.04' : - 'ubuntu:20.04' }" + 'quay.io/nf-core/ubuntu:20.04' }" input: tuple val(meta), path(vpairs) diff --git a/modules/local/hicpro/run_ice.nf b/modules/local/hicpro/run_ice.nf index 0a435edcd0f2a82c93be2a1b9bd832a721c270cf..74c96dc828399461d725fc52752cf98337b5dc02 100644 --- a/modules/local/hicpro/run_ice.nf +++ b/modules/local/hicpro/run_ice.nf @@ -5,7 +5,7 @@ process ICE_NORMALIZATION{ conda "conda-forge::python=3.9 bioconda::iced=0.5.10 conda-forge::numpy=1.22.3" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/mulled-v2-c6ff206325681cbb9c9ef890bb8de554172c0483:713df51cd897ceb893b9a6e6420f527d83c2ed95-0' : - 'quay.io/biocontainers/mulled-v2-c6ff206325681cbb9c9ef890bb8de554172c0483:713df51cd897ceb893b9a6e6420f527d83c2ed95-0'}" + 'biocontainers/mulled-v2-c6ff206325681cbb9c9ef890bb8de554172c0483:713df51cd897ceb893b9a6e6420f527d83c2ed95-0'}" input: tuple val(meta), val(res), path(rmaps), path(bed) diff --git a/modules/local/hicpro/trim_reads.nf b/modules/local/hicpro/trim_reads.nf index 5a96df412ea89845583fc363eccf2df49ba9458d..b9f2ea47e8f2ac40ae3a8ec17f6e01d0e22401b3 100644 --- a/modules/local/hicpro/trim_reads.nf +++ b/modules/local/hicpro/trim_reads.nf @@ -5,7 +5,7 @@ process TRIM_READS { conda "conda-forge::sed=4.7" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/ubuntu:20.04' : - 'ubuntu:20.04' }" + 'quay.io/nf-core/ubuntu:20.04' }" input: tuple val(meta), path(reads) diff --git a/modules/local/multiqc.nf b/modules/local/multiqc.nf index 595dc94d0eebd849d217c25349e4bb253bee64b6..6f6bad458bb8d83962f1d5a1bc922caeb7d3d9f9 100644 --- a/modules/local/multiqc.nf +++ b/modules/local/multiqc.nf @@ -4,7 +4,7 @@ process MULTIQC { conda "bioconda::multiqc=1.13" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/multiqc:1.13--pyhdfd78af_0' : - 'quay.io/biocontainers/multiqc:1.13--pyhdfd78af_0' }" + 'biocontainers/multiqc:1.13--pyhdfd78af_0' }" input: path multiqc_config diff --git a/modules/local/samplesheet_check.nf b/modules/local/samplesheet_check.nf index 1624dfafe6425f07bbd15daf3b2735ea1187c0ec..096c80b0bd37b9a40bbf61cdefa13007f58b0c5d 100644 --- a/modules/local/samplesheet_check.nf +++ b/modules/local/samplesheet_check.nf @@ -5,7 +5,7 @@ process SAMPLESHEET_CHECK { conda "conda-forge::python=3.8.3" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/python:3.8.3' : - 'quay.io/biocontainers/python:3.8.3' }" + 'biocontainers/python:3.8.3' }" input: path samplesheet diff --git a/modules/local/split_cooler_dump.nf b/modules/local/split_cooler_dump.nf index 8b9e45b31d304545ea62946f4c4e0eacb7860002..5e392c140a86e51925b1ce751a81868dda5268ef 100644 --- a/modules/local/split_cooler_dump.nf +++ b/modules/local/split_cooler_dump.nf @@ -5,7 +5,7 @@ process SPLIT_COOLER_DUMP { conda "conda-forge::gawk=5.1.0" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/ubuntu:20.04' : - 'ubuntu:20.04' }" + 'quay.io/nf-core/ubuntu:20.04' }" input: tuple val(meta), path(bedpe) diff --git a/modules/nf-core/bowtie2/align/main.nf b/modules/nf-core/bowtie2/align/main.nf index 3d851866a30b4f629881864516f0789f6cae9443..311a1505eccdd3825db6ad8652305cb445148ae3 100644 --- a/modules/nf-core/bowtie2/align/main.nf +++ b/modules/nf-core/bowtie2/align/main.nf @@ -5,7 +5,7 @@ process BOWTIE2_ALIGN { conda "bioconda::bowtie2=2.4.4 bioconda::samtools=1.16.1 conda-forge::pigz=2.6" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/mulled-v2-ac74a7f02cebcfcc07d8e8d1d750af9c83b4d45a:a0ffedb52808e102887f6ce600d092675bf3528a-0' : - 'quay.io/biocontainers/mulled-v2-ac74a7f02cebcfcc07d8e8d1d750af9c83b4d45a:a0ffedb52808e102887f6ce600d092675bf3528a-0' }" + 'biocontainers/mulled-v2-ac74a7f02cebcfcc07d8e8d1d750af9c83b4d45a:a0ffedb52808e102887f6ce600d092675bf3528a-0' }" input: tuple val(meta) , path(reads) diff --git a/modules/nf-core/bowtie2/build/main.nf b/modules/nf-core/bowtie2/build/main.nf index 551893af3b3bb1a3955f1cc7c0b50735c6fded8d..566a4accda99ed7c9c56564f6c3348bec439df11 100644 --- a/modules/nf-core/bowtie2/build/main.nf +++ b/modules/nf-core/bowtie2/build/main.nf @@ -5,7 +5,7 @@ process BOWTIE2_BUILD { conda "bioconda::bowtie2=2.4.4" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/bowtie2:2.4.4--py39hbb4e92a_0' : - 'quay.io/biocontainers/bowtie2:2.4.4--py39hbb4e92a_0' }" + 'biocontainers/bowtie2:2.4.4--py39hbb4e92a_0' }" input: tuple val(meta), path(fasta) diff --git a/modules/nf-core/cooler/balance/main.nf b/modules/nf-core/cooler/balance/main.nf index 4173a3c1291df7e25e3529d9ab8f2b6f8d1240b7..6e131d5b4717de6a6e7079b067154f7dd7e4e937 100644 --- a/modules/nf-core/cooler/balance/main.nf +++ b/modules/nf-core/cooler/balance/main.nf @@ -5,7 +5,7 @@ process COOLER_BALANCE { conda "bioconda::cooler=0.8.11" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/cooler:0.8.11--pyh3252c3a_0': - 'quay.io/biocontainers/cooler:0.8.11--pyh3252c3a_0' }" + 'biocontainers/cooler:0.8.11--pyh3252c3a_0' }" input: tuple val(meta), path(cool), val(resolution) diff --git a/modules/nf-core/cooler/cload/main.nf b/modules/nf-core/cooler/cload/main.nf index 80d61f07336f81233a6bd8ffb116e5a25e57048d..80109d48f449af278016bbda6cd08c46bbf8e183 100644 --- a/modules/nf-core/cooler/cload/main.nf +++ b/modules/nf-core/cooler/cload/main.nf @@ -5,7 +5,7 @@ process COOLER_CLOAD { conda "bioconda::cooler=0.8.11" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/cooler:0.8.11--pyh3252c3a_0' : - 'quay.io/biocontainers/cooler:0.8.11--pyh3252c3a_0' }" + 'biocontainers/cooler:0.8.11--pyh3252c3a_0' }" input: tuple val(meta), path(pairs), path(index), val(cool_bin) diff --git a/modules/nf-core/cooler/dump/main.nf b/modules/nf-core/cooler/dump/main.nf index b46c78cf35ec6b40276e2d792635f97b9909ab55..fed7502f8cb6a788b056c87aab3a2e71d26dd400 100644 --- a/modules/nf-core/cooler/dump/main.nf +++ b/modules/nf-core/cooler/dump/main.nf @@ -5,7 +5,7 @@ process COOLER_DUMP { conda "bioconda::cooler=0.8.11" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/cooler:0.8.11--pyh3252c3a_0' : - 'quay.io/biocontainers/cooler:0.8.11--pyh3252c3a_0' }" + 'biocontainers/cooler:0.8.11--pyh3252c3a_0' }" input: tuple val(meta), path(cool), val(resolution) diff --git a/modules/nf-core/cooler/makebins/main.nf b/modules/nf-core/cooler/makebins/main.nf index 7f0826197e99e9e79107359a8c65d7d60346dc49..25d6a40f636257c90251893c74eb2769200b41c1 100644 --- a/modules/nf-core/cooler/makebins/main.nf +++ b/modules/nf-core/cooler/makebins/main.nf @@ -5,7 +5,7 @@ process COOLER_MAKEBINS { conda "bioconda::cooler=0.8.11" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/cooler:0.8.11--pyh3252c3a_0': - 'quay.io/biocontainers/cooler:0.8.11--pyh3252c3a_0' }" + 'biocontainers/cooler:0.8.11--pyh3252c3a_0' }" input: tuple val(meta), path(chromsizes), val(cool_bin) diff --git a/modules/nf-core/cooler/zoomify/main.nf b/modules/nf-core/cooler/zoomify/main.nf index f1cd8df79d02b8630c31de0620521cef0cdd6df0..95e7daff0d19429507d01033eb78eba491625220 100644 --- a/modules/nf-core/cooler/zoomify/main.nf +++ b/modules/nf-core/cooler/zoomify/main.nf @@ -5,7 +5,7 @@ process COOLER_ZOOMIFY { conda "bioconda::cooler=0.8.11" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/cooler:0.8.11--pyh3252c3a_0' : - 'quay.io/biocontainers/cooler:0.8.11--pyh3252c3a_0' }" + 'biocontainers/cooler:0.8.11--pyh3252c3a_0' }" input: tuple val(meta), path(cool) diff --git a/modules/nf-core/custom/dumpsoftwareversions/main.nf b/modules/nf-core/custom/dumpsoftwareversions/main.nf index 3df21765b90921413962c3bb5ca44d117d829297..ebc8727339b0ee4ce7e2feb981a0aaf416723b57 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/main.nf +++ b/modules/nf-core/custom/dumpsoftwareversions/main.nf @@ -2,10 +2,10 @@ process CUSTOM_DUMPSOFTWAREVERSIONS { label 'process_single' // Requires `pyyaml` which does not have a dedicated container but is in the MultiQC container - conda "bioconda::multiqc=1.13" + conda "bioconda::multiqc=1.14" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.13--pyhdfd78af_0' : - 'quay.io/biocontainers/multiqc:1.13--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.14--pyhdfd78af_0' : + 'biocontainers/multiqc:1.14--pyhdfd78af_0' }" input: path versions diff --git a/modules/nf-core/custom/dumpsoftwareversions/meta.yml b/modules/nf-core/custom/dumpsoftwareversions/meta.yml index 60b546a012c457b5459490e732e6ac9be6979db1..c32657de7a2c09e82da1e753a234300fb76237ed 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/meta.yml +++ b/modules/nf-core/custom/dumpsoftwareversions/meta.yml @@ -1,7 +1,9 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/yaml-schema.json name: custom_dumpsoftwareversions description: Custom module used to dump software versions within the nf-core pipeline template keywords: - custom + - dump - version tools: - custom: diff --git a/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py b/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py index b83b32c4d4242764a439f4d51e0255711f9cd40f..da03340857c4c90957c79c9f892030bc1bb397a3 100755 --- a/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py +++ b/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py @@ -3,10 +3,10 @@ """Provide functions to merge multiple versions.yml files.""" -import platform -from textwrap import dedent import yaml +import platform +from textwrap import dedent def _make_versions_html(versions): diff --git a/modules/nf-core/custom/getchromsizes/main.nf b/modules/nf-core/custom/getchromsizes/main.nf index 580f87feade7280bb8d520551aa61f0021b3b88d..060a2e88527af75fabb1d87858c2885b6ca09547 100644 --- a/modules/nf-core/custom/getchromsizes/main.nf +++ b/modules/nf-core/custom/getchromsizes/main.nf @@ -5,7 +5,7 @@ process CUSTOM_GETCHROMSIZES { conda "bioconda::samtools=1.16.1" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/samtools:1.16.1--h6899075_1' : - 'quay.io/biocontainers/samtools:1.16.1--h6899075_1' }" + 'biocontainers/samtools:1.16.1--h6899075_1' }" input: tuple val(meta), path(fasta) diff --git a/modules/nf-core/fastqc/main.nf b/modules/nf-core/fastqc/main.nf index 9ae5838158b28d2ae49270133fbbfe0ea673e991..07d5e433128b416714860fe1dca659219ba1d1ad 100644 --- a/modules/nf-core/fastqc/main.nf +++ b/modules/nf-core/fastqc/main.nf @@ -5,7 +5,7 @@ process FASTQC { conda "bioconda::fastqc=0.11.9" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/fastqc:0.11.9--0' : - 'quay.io/biocontainers/fastqc:0.11.9--0' }" + 'biocontainers/fastqc:0.11.9--0' }" input: tuple val(meta), path(reads) diff --git a/nextflow.config b/nextflow.config index 9313b6a2312b28821180d9f3a38595d9b1312994..40507dd642c9a0dcd4fba6320ed5e750e1152445 100644 --- a/nextflow.config +++ b/nextflow.config @@ -54,13 +54,13 @@ params { ligation_site='GATCGATC,GATCANTC,GANTGATC,GANTANTC' } } - + min_restriction_fragment_size = 0 max_restriction_fragment_size = 0 min_insert_size = 0 max_insert_size = 0 save_pairs_intermediates = false - + // Dnase Hi-C dnase = false min_cis_dist = 0 @@ -111,7 +111,7 @@ params { validate_params = true show_hidden_params = false schema_ignore_params = 'genomes,digest' - + // Config options custom_config_version = 'master' custom_config_base = "https://raw.githubusercontent.com/nf-core/configs/${params.custom_config_version}" @@ -242,6 +242,12 @@ env { // Capture exit codes from upstream processes when piping process.shell = ['/bin/bash', '-euo', 'pipefail'] +// Set default registry for Docker and Podman independent of -profile +// Will not be used unless Docker / Podman are enabled +// Set to your registry if you have a mirror of containers +docker.registry = 'quay.io' +podman.registry = 'quay.io' + def trace_timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') timeline { enabled = true