diff --git a/6_dea/dea.Rmd b/6_dea/dea.Rmd index 9007425b9adbba810a56010c51579ae93773d999..bb790f7c5f50b2062860dd857b5184b15c306231 100644 --- a/6_dea/dea.Rmd +++ b/6_dea/dea.Rmd @@ -21,26 +21,12 @@ classoption: aspectratio=169 ## Program -1. Single-cell RNASeq data from 10X Sequencing (Friday 3 June 2022 - 14:00) -2. Normalization and spurious effects (Wednesday 8 June 2022 - 14:00) -3. Dimension reduction and data visualization (Monday 13 June 2022 - 15:00) -4. Clustering and annotation (Thursday 30 June 2022 2022 - 14:00) -5. Pseudo-time and velocity inference (Friday 8 July 2022 - 14:00) 6. Differential expression analysis (Monday 11 July 2022 - 15:30) - -## Program - -1. Single-cell RNASeq data from 10X Sequencing (Friday 3 June 2022 - 14:00) -2. Normalization and spurious effects (Wednesday 8 June 2022 - 14:00) -3. Dimension reduction and data visualization (Monday 13 June 2022 - 15:00) -4. Clustering and annotation (Thursday 30 June 2022 2022 - 14:00) -5. Pseudo-time and velocity inference (Friday 8 July 2022 - 14:00) - Parametric versus non-parametric testing - Differential expression analysis between groups - Regression analysis - Multiple testing - Multivariate Differential expression analysis -6. Differential expression analysis (Monday 11 July 2022 - 15:30) # Hypothesis testing @@ -58,8 +44,9 @@ classoption: aspectratio=169 \item $H_1$: the gene pression is not the same between the $2$ groups \end{itemize} + \begin{center} - \bf We reject $H_0$ with a given risk $\alpha$ and a power $\beta$ + {\bf We reject $H_0$ with a given risk $\alpha$ and a power $\beta$} \end{center} ## Hypothesis testing @@ -74,11 +61,10 @@ classoption: aspectratio=169 ## Hypothesis testing \begin{center} - \href{https://en.wikipedia.org/wiki/Power_of_a_test#/media/File:Statistical_test,_significance_level,_power.png}{ + \href{https://en.wikipedia.org/}{ \includegraphics[width=0.6\textwidth]{img/alpha_beta.png} } \end{center} - # Parametric versus non-parametric testing # Differential expression analysis between groups