diff --git a/src/nf_modules/htseq/main.nf b/src/nf_modules/htseq/main.nf
index e7fb52d5c37f25bf35eaaa21b420a334be5074cc..723c8b840cba6aaef8a3f8c21b2a5cd8fb44407d 100644
--- a/src/nf_modules/htseq/main.nf
+++ b/src/nf_modules/htseq/main.nf
@@ -36,7 +36,7 @@ process htseq_count {
 
   script:
 """
-htseq-count -n ${task.cpus} -r pos -a 10 -s reverse -t exon -i gene_id $bam $gtf_file > ${file_id}.tsv
+htseq-count -n ${task.cpus} -r pos -a 10 -s no -t exon -i gene_id $bam $gtf_file > ${file_id}.tsv
 """
 }