diff --git a/src/nf_modules/FastQC/fastqc.config b/src/nf_modules/FastQC/fastqc.config index db3b0270190b5e736a709774713ac3115b5fa6ba..a6589845aa98db0b4f6eaa663246db2604eb210b 100644 --- a/src/nf_modules/FastQC/fastqc.config +++ b/src/nf_modules/FastQC/fastqc.config @@ -3,14 +3,14 @@ profiles { docker.temp = 'auto' docker.enabled = true process { - $fastqc { + $fastqc_fastq { container = "fastqc:0.11.5" } } } sge { process{ - $fastqc { + $fastqc_fastq { beforeScript = "module purge; module load FastQC/0.11.5" executor = "sge" cpus = 1 diff --git a/src/nf_modules/FastQC/fastqc.nf b/src/nf_modules/FastQC/fastqc.nf index abe18a49fbf4f17e2ff2a538bc20f34bf0796c51..6ab41409a5f505cd9de5bb2c4e6c9b658a8bc654 100644 --- a/src/nf_modules/FastQC/fastqc.nf +++ b/src/nf_modules/FastQC/fastqc.nf @@ -5,7 +5,13 @@ */ /* fastQC */ -params.fastq = "" + + +/* +* for single-end data +*/ + +params.fastq = "$baseDir/data/fastq/*.fastq" log.info "fastq files : ${params.fastq}" @@ -14,20 +20,51 @@ Channel .ifEmpty { error "Cannot find any fastq files matching: ${params.fastq}" } .set { fastq_files } -process fastqc { +process fastqc_fastq { tag "$fastq.baseName" - publishDir "results/fastqc/", mode: 'copy' + publishDir "results/fastq/fastqc/", mode: 'copy' + cpus = 1 input: file fastq from fastq_files output: - file "*.htlm" into fastqc_repport + file "*.{zip,html}" into fastqc_repport script: """ -fastqc -o ./ --noextract -f fastq ${fastq} +fastqc --quiet --threads ${task.cpus} --outdir -f fastq ./ ${fastq} """ } +/* +* for paired-end data +*/ + +params.fastq = "$baseDir/data/fastq/*_{1,2}.fastq" + +log.info "fastq files : ${params.fastq}" + +Channel + .fromFilePairs( params.fastq ) + .ifEmpty { error "Cannot find any fastq files matching: ${params.fastq}" } + .set { fastq_files } + +process fastqc_fastq { + tag "$pair_id" + publishDir "results/fastq/fastqc/", mode: 'copy' + + input: + set pair_id, file(reads) from fastq_files + + output: + file "*.{zip,html}" into fastqc_repport + + script: +""" +fastqc --quiet --threads ${task.cpus} --outdir -f fastq ./ \ +${fastq[0]} ${fastq[1]} +""" +} +