diff --git a/src/nf_modules/FastQC/fastqc.nf b/src/nf_modules/FastQC/fastqc.nf index 6ab41409a5f505cd9de5bb2c4e6c9b658a8bc654..5a8237cc06b5a94fdf9a219a69ab3413edc7977e 100644 --- a/src/nf_modules/FastQC/fastqc.nf +++ b/src/nf_modules/FastQC/fastqc.nf @@ -21,19 +21,19 @@ Channel .set { fastq_files } process fastqc_fastq { - tag "$fastq.baseName" + tag "$reads.baseName" publishDir "results/fastq/fastqc/", mode: 'copy' cpus = 1 input: - file fastq from fastq_files + file reads from fastq_files output: file "*.{zip,html}" into fastqc_repport script: """ -fastqc --quiet --threads ${task.cpus} --outdir -f fastq ./ ${fastq} +fastqc --quiet --threads ${task.cpus} --format fastq --outdir ./ ${reads} """ } @@ -63,8 +63,8 @@ process fastqc_fastq { script: """ -fastqc --quiet --threads ${task.cpus} --outdir -f fastq ./ \ -${fastq[0]} ${fastq[1]} +fastqc --quiet --threads ${task.cpus} --format fastq --outdir ./ \ +${reads[0]} ${reads[1]} """ } diff --git a/src/nf_modules/FastQC/tests/fastqc_paired.nf b/src/nf_modules/FastQC/tests/fastqc_paired.nf new file mode 100644 index 0000000000000000000000000000000000000000..d7cec0ddece0a40098bb74cf09b7ab22f4a5b207 --- /dev/null +++ b/src/nf_modules/FastQC/tests/fastqc_paired.nf @@ -0,0 +1,26 @@ +params.fastq = "$baseDir/data/fastq/*_{1,2}.fastq" + +log.info "fastq files : ${params.fastq}" + +Channel + .fromFilePairs( params.fastq ) + .ifEmpty { error "Cannot find any fastq files matching: ${params.fastq}" } + .set { fastq_files } + +process fastqc_fastq { + tag "$pair_id" + publishDir "results/fastq/fastqc/", mode: 'copy' + + input: + set pair_id, file(reads) from fastq_files + + output: + file "*.{zip,html}" into fastqc_repport + + script: +""" +fastqc --quiet --threads ${task.cpus} --format fastq --outdir ./ \ +${reads[0]} ${reads[1]} +""" +} + diff --git a/src/nf_modules/FastQC/tests/fastqc_single.nf b/src/nf_modules/FastQC/tests/fastqc_single.nf new file mode 100644 index 0000000000000000000000000000000000000000..1ec9ad3dc08728c84690230975817efea990cda9 --- /dev/null +++ b/src/nf_modules/FastQC/tests/fastqc_single.nf @@ -0,0 +1,26 @@ +params.fastq = "$baseDir/data/fastq/*.fastq" + +log.info "fastq files : ${params.fastq}" + +Channel + .fromPath( params.fastq ) + .ifEmpty { error "Cannot find any fastq files matching: ${params.fastq}" } + .set { fastq_files } + +process fastqc_fastq { + tag "$reads.baseName" + publishDir "results/fastq/fastqc/", mode: 'copy' + cpus = 1 + + input: + file reads from fastq_files + + output: + file "*.{zip,html}" into fastqc_repport + + script: +""" +fastqc --quiet --threads ${task.cpus} --format fastq --outdir ./ ${reads} +""" +} + diff --git a/src/nf_modules/FastQC/tests/tests.sh b/src/nf_modules/FastQC/tests/tests.sh new file mode 100755 index 0000000000000000000000000000000000000000..147503b411bd0232f064c76fb75c1a45533a0e79 --- /dev/null +++ b/src/nf_modules/FastQC/tests/tests.sh @@ -0,0 +1,9 @@ +nextflow src/nf_modules/FastQC/tests/fastqc_paired.nf \ + -c src/nf_modules/FastQC/fastqc.config \ + -profile docker \ + --fastq "data/tiny_dataset/fastq/tiny_R{1,2}.fastq" + +nextflow src/nf_modules/FastQC/tests/fastqc_single.nf \ + -c src/nf_modules/FastQC/fastqc.config \ + -profile docker \ + --fastq "data/tiny_dataset/fastq/tiny_S.fastq"